Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1937 and GFF1938 overlap by 4 nucleotides GFF1938 and GFF1939 are separated by 179 nucleotides GFF1939 and GFF1940 are separated by 172 nucleotides
GFF1937 - Cell division protein, at 129,985 to 130,986
GFF1937
GFF1938 - Transporter, putative, at 130,983 to 132,161
GFF1938
GFF1939 - FIG00643583: hypothetical protein, at 132,341 to 132,715
GFF1939
GFF1940 - Putative DNA-binding protein in cluster with Type I restriction-modification system, at 132,888 to 133,136
GFF1940
Position (kb) 130
131
132
133 Strain fitness (log2 ratio) -1
0
1 at 129.984 kb on + strand at 129.984 kb on + strand at 130.143 kb on - strand, within GFF1937 at 130.287 kb on - strand, within GFF1937 at 130.486 kb on - strand, within GFF1937 at 130.612 kb on - strand, within GFF1937 at 130.655 kb on + strand, within GFF1937 at 130.673 kb on + strand, within GFF1937 at 130.677 kb on + strand, within GFF1937 at 130.677 kb on + strand, within GFF1937 at 130.678 kb on - strand, within GFF1937 at 130.738 kb on + strand, within GFF1937 at 130.738 kb on + strand, within GFF1937 at 130.739 kb on - strand, within GFF1937 at 130.806 kb on + strand, within GFF1937 at 130.829 kb on + strand, within GFF1937 at 130.959 kb on - strand at 131.002 kb on - strand at 131.052 kb on + strand at 131.169 kb on + strand, within GFF1938 at 131.170 kb on - strand, within GFF1938 at 131.447 kb on + strand, within GFF1938 at 131.511 kb on - strand, within GFF1938 at 131.816 kb on + strand, within GFF1938 at 131.817 kb on - strand, within GFF1938 at 132.012 kb on - strand, within GFF1938 at 132.079 kb on + strand at 132.079 kb on + strand at 132.080 kb on - strand at 132.211 kb on + strand at 132.257 kb on - strand at 132.280 kb on - strand at 132.486 kb on - strand, within GFF1939 at 132.543 kb on + strand, within GFF1939 at 132.562 kb on + strand, within GFF1939 at 132.631 kb on - strand, within GFF1939 at 133.101 kb on - strand, within GFF1940
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 129,984 + +0.6 129,984 + +1.2 130,143 - GFF1937 0.16 +0.2 130,287 - GFF1937 0.30 -0.3 130,486 - GFF1937 0.50 +0.3 130,612 - GFF1937 0.63 +0.5 130,655 + GFF1937 0.67 -0.9 130,673 + GFF1937 0.69 +0.7 130,677 + GFF1937 0.69 +0.3 130,677 + GFF1937 0.69 -0.3 130,678 - GFF1937 0.69 -0.6 130,738 + GFF1937 0.75 +0.5 130,738 + GFF1937 0.75 +1.3 130,739 - GFF1937 0.75 -0.2 130,806 + GFF1937 0.82 -0.0 130,829 + GFF1937 0.84 -0.3 130,959 - +1.3 131,002 - +0.2 131,052 + +0.1 131,169 + GFF1938 0.16 -0.2 131,170 - GFF1938 0.16 +0.3 131,447 + GFF1938 0.39 -0.7 131,511 - GFF1938 0.45 -0.4 131,816 + GFF1938 0.71 -0.1 131,817 - GFF1938 0.71 -1.4 132,012 - GFF1938 0.87 +0.5 132,079 + -0.2 132,079 + -0.3 132,080 - -0.2 132,211 + +0.1 132,257 - +0.1 132,280 - +0.9 132,486 - GFF1939 0.39 +0.1 132,543 + GFF1939 0.54 +0.2 132,562 + GFF1939 0.59 +0.1 132,631 - GFF1939 0.77 +1.1 133,101 - GFF1940 0.86 -0.5
Or see this region's nucleotide sequence