Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF99 and GFF100 are separated by 116 nucleotides GFF100 and GFF101 overlap by 4 nucleotides
GFF99 - Putative fimbriae, at 84,020 to 85,099
GFF99
GFF100 - ABC-type multidrug transport system, permease component, at 85,216 to 85,986
GFF100
GFF101 - ABC-type multidrug transport system, ATPase component, at 85,983 to 86,909
GFF101
Position (kb) 85
86 Strain fitness (log2 ratio) -1
0
1 at 84.225 kb on - strand, within GFF99 at 84.268 kb on + strand, within GFF99 at 84.272 kb on + strand, within GFF99 at 84.419 kb on + strand, within GFF99 at 84.420 kb on - strand, within GFF99 at 84.420 kb on - strand, within GFF99 at 84.421 kb on + strand, within GFF99 at 84.449 kb on - strand, within GFF99 at 84.530 kb on + strand, within GFF99 at 84.531 kb on - strand, within GFF99 at 84.531 kb on - strand, within GFF99 at 84.533 kb on - strand, within GFF99 at 84.536 kb on - strand, within GFF99 at 84.550 kb on - strand, within GFF99 at 84.581 kb on - strand, within GFF99 at 84.591 kb on - strand, within GFF99 at 84.622 kb on + strand, within GFF99 at 84.622 kb on + strand, within GFF99 at 84.623 kb on - strand, within GFF99 at 84.625 kb on + strand, within GFF99 at 84.626 kb on - strand, within GFF99 at 84.814 kb on + strand, within GFF99 at 84.815 kb on - strand, within GFF99 at 84.941 kb on - strand, within GFF99 at 84.941 kb on - strand, within GFF99 at 85.061 kb on + strand at 85.266 kb on - strand at 85.362 kb on - strand, within GFF100 at 85.422 kb on - strand, within GFF100 at 85.568 kb on - strand, within GFF100 at 85.627 kb on + strand, within GFF100 at 85.767 kb on + strand, within GFF100 at 85.768 kb on - strand, within GFF100 at 85.771 kb on - strand, within GFF100 at 85.771 kb on - strand, within GFF100 at 85.804 kb on - strand, within GFF100 at 85.814 kb on + strand, within GFF100 at 85.814 kb on + strand, within GFF100 at 85.815 kb on - strand, within GFF100 at 86.048 kb on + strand at 86.051 kb on + strand at 86.052 kb on - strand at 86.057 kb on + strand at 86.057 kb on + strand at 86.147 kb on + strand, within GFF101 at 86.147 kb on + strand, within GFF101 at 86.161 kb on + strand, within GFF101 at 86.174 kb on + strand, within GFF101 at 86.181 kb on - strand, within GFF101 at 86.203 kb on - strand, within GFF101 at 86.289 kb on - strand, within GFF101 at 86.566 kb on - strand, within GFF101 at 86.613 kb on + strand, within GFF101 at 86.760 kb on - strand, within GFF101 at 86.922 kb on - strand at 86.945 kb on + strand at 86.946 kb on - strand at 86.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 84,225 - GFF99 0.19 -0.4 84,268 + GFF99 0.23 -0.4 84,272 + GFF99 0.23 +0.2 84,419 + GFF99 0.37 -0.9 84,420 - GFF99 0.37 -0.0 84,420 - GFF99 0.37 +0.2 84,421 + GFF99 0.37 +0.5 84,449 - GFF99 0.40 -0.2 84,530 + GFF99 0.47 +0.9 84,531 - GFF99 0.47 +0.6 84,531 - GFF99 0.47 +0.2 84,533 - GFF99 0.47 +0.1 84,536 - GFF99 0.48 -0.4 84,550 - GFF99 0.49 -0.1 84,581 - GFF99 0.52 +0.5 84,591 - GFF99 0.53 -0.2 84,622 + GFF99 0.56 +0.2 84,622 + GFF99 0.56 -0.3 84,623 - GFF99 0.56 -0.1 84,625 + GFF99 0.56 +0.4 84,626 - GFF99 0.56 -0.6 84,814 + GFF99 0.74 -0.2 84,815 - GFF99 0.74 +0.8 84,941 - GFF99 0.85 +0.4 84,941 - GFF99 0.85 -0.1 85,061 + -0.6 85,266 - -0.1 85,362 - GFF100 0.19 +1.0 85,422 - GFF100 0.27 +0.3 85,568 - GFF100 0.46 -0.6 85,627 + GFF100 0.53 -0.4 85,767 + GFF100 0.71 +0.7 85,768 - GFF100 0.72 +0.0 85,771 - GFF100 0.72 -0.0 85,771 - GFF100 0.72 +0.1 85,804 - GFF100 0.76 -0.2 85,814 + GFF100 0.78 +0.5 85,814 + GFF100 0.78 +0.2 85,815 - GFF100 0.78 -0.4 86,048 + +0.0 86,051 + -0.1 86,052 - +1.2 86,057 + +1.4 86,057 + -0.1 86,147 + GFF101 0.18 +0.2 86,147 + GFF101 0.18 -0.3 86,161 + GFF101 0.19 -0.4 86,174 + GFF101 0.21 -0.2 86,181 - GFF101 0.21 +0.1 86,203 - GFF101 0.24 +0.1 86,289 - GFF101 0.33 -0.1 86,566 - GFF101 0.63 +0.4 86,613 + GFF101 0.68 -0.2 86,760 - GFF101 0.84 -0.2 86,922 - -0.1 86,945 + +0.5 86,946 - +0.6 86,946 - +0.2
Or see this region's nucleotide sequence