Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF819 and GFF820 overlap by 14 nucleotides GFF820 and GFF821 overlap by 26 nucleotides GFF821 and GFF822 are separated by 16 nucleotides
GFF819 - Epoxyqueuosine (oQ) reductase QueG, at 579 to 1,733
GFF819
GFF820 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 1,720 to 3,264
GFF820
GFF821 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 3,239 to 3,697
GFF821
GFF822 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 3,714 to 5,033
GFF822
Position (kb) 1
2
3
4 Strain fitness (log2 ratio) -1
0
1 at 0.808 kb on - strand, within GFF819 at 0.873 kb on - strand, within GFF819 at 0.917 kb on + strand, within GFF819 at 0.966 kb on - strand, within GFF819 at 0.992 kb on + strand, within GFF819 at 1.055 kb on - strand, within GFF819 at 1.061 kb on + strand, within GFF819 at 1.232 kb on + strand, within GFF819 at 1.244 kb on + strand, within GFF819 at 1.245 kb on - strand, within GFF819 at 1.336 kb on + strand, within GFF819 at 1.380 kb on + strand, within GFF819 at 1.381 kb on - strand, within GFF819 at 1.381 kb on - strand, within GFF819 at 1.474 kb on + strand, within GFF819 at 1.635 kb on - strand at 1.638 kb on - strand at 1.748 kb on + strand at 1.748 kb on + strand at 1.749 kb on - strand at 1.752 kb on + strand at 1.752 kb on + strand at 1.754 kb on + strand at 1.885 kb on + strand, within GFF820 at 1.886 kb on - strand, within GFF820 at 1.945 kb on + strand, within GFF820 at 2.348 kb on + strand, within GFF820 at 2.467 kb on - strand, within GFF820 at 2.576 kb on + strand, within GFF820 at 2.633 kb on + strand, within GFF820 at 2.634 kb on - strand, within GFF820 at 2.763 kb on + strand, within GFF820 at 2.763 kb on + strand, within GFF820 at 2.763 kb on + strand, within GFF820 at 2.763 kb on + strand, within GFF820 at 2.901 kb on - strand, within GFF820 at 2.922 kb on - strand, within GFF820 at 3.230 kb on + strand at 3.699 kb on + strand at 3.699 kb on + strand at 3.700 kb on - strand at 3.742 kb on + strand at 3.751 kb on + strand at 3.752 kb on - strand at 3.830 kb on + strand at 3.865 kb on - strand, within GFF822 at 4.068 kb on + strand, within GFF822 at 4.069 kb on - strand, within GFF822 at 4.069 kb on - strand, within GFF822
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 808 - GFF819 0.20 -0.0 873 - GFF819 0.25 -0.1 917 + GFF819 0.29 +0.3 966 - GFF819 0.34 -0.4 992 + GFF819 0.36 -0.2 1,055 - GFF819 0.41 -0.7 1,061 + GFF819 0.42 -0.5 1,232 + GFF819 0.57 -0.1 1,244 + GFF819 0.58 -0.6 1,245 - GFF819 0.58 -0.3 1,336 + GFF819 0.66 +0.1 1,380 + GFF819 0.69 +0.3 1,381 - GFF819 0.69 +0.2 1,381 - GFF819 0.69 -0.5 1,474 + GFF819 0.77 +0.8 1,635 - -0.7 1,638 - +0.1 1,748 + -0.0 1,748 + +0.6 1,749 - +0.0 1,752 + -1.4 1,752 + +0.1 1,754 + +0.1 1,885 + GFF820 0.11 +0.4 1,886 - GFF820 0.11 +0.1 1,945 + GFF820 0.15 -1.6 2,348 + GFF820 0.41 -0.1 2,467 - GFF820 0.48 +0.2 2,576 + GFF820 0.55 -0.1 2,633 + GFF820 0.59 -0.1 2,634 - GFF820 0.59 -0.1 2,763 + GFF820 0.68 +0.0 2,763 + GFF820 0.68 -0.1 2,763 + GFF820 0.68 -0.1 2,763 + GFF820 0.68 +0.1 2,901 - GFF820 0.76 -0.6 2,922 - GFF820 0.78 +0.1 3,230 + -0.8 3,699 + +0.0 3,699 + +0.4 3,700 - -0.4 3,742 + +0.6 3,751 + +0.3 3,752 - -0.8 3,830 + -0.1 3,865 - GFF822 0.11 -0.3 4,068 + GFF822 0.27 +0.2 4,069 - GFF822 0.27 +0.0 4,069 - GFF822 0.27 -0.1
Or see this region's nucleotide sequence