Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF78 and GFF79 overlap by 4 nucleotides GFF79 and GFF80 overlap by 1 nucleotides GFF80 and GFF81 are separated by 48 nucleotides
GFF78 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), permease component FhuB, at 59,372 to 61,429
GFF78
GFF79 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), periplasmic substrate binding protein FhuD, at 61,426 to 62,316
GFF79
GFF80 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), ATP-binding protein FhuC, at 62,316 to 63,113
GFF80
GFF81 - Ferric hydroxamate outer membrane receptor FhuA, at 63,162 to 65,273
GFF81
Position (kb) 61
62
63 Strain fitness (log2 ratio) -1
0
1 at 60.473 kb on - strand, within GFF78 at 60.866 kb on + strand, within GFF78 at 61.110 kb on - strand, within GFF78 at 61.235 kb on - strand at 61.374 kb on - strand at 61.374 kb on - strand at 61.449 kb on + strand at 61.493 kb on + strand at 61.493 kb on + strand at 61.494 kb on - strand at 61.626 kb on + strand, within GFF79 at 61.627 kb on - strand, within GFF79 at 61.628 kb on + strand, within GFF79 at 61.629 kb on - strand, within GFF79 at 61.755 kb on + strand, within GFF79 at 61.756 kb on - strand, within GFF79 at 61.845 kb on - strand, within GFF79 at 61.845 kb on - strand, within GFF79 at 61.945 kb on - strand, within GFF79 at 62.123 kb on + strand, within GFF79 at 62.124 kb on - strand, within GFF79 at 62.281 kb on + strand at 62.295 kb on + strand at 62.304 kb on - strand at 62.317 kb on + strand at 62.318 kb on - strand at 62.318 kb on - strand at 62.320 kb on + strand at 62.320 kb on + strand at 62.381 kb on - strand at 62.464 kb on + strand, within GFF80 at 62.704 kb on - strand, within GFF80 at 62.730 kb on + strand, within GFF80 at 62.848 kb on + strand, within GFF80 at 62.966 kb on + strand, within GFF80 at 62.987 kb on + strand, within GFF80 at 62.988 kb on - strand, within GFF80 at 63.087 kb on - strand at 63.220 kb on + strand at 63.263 kb on + strand at 63.316 kb on + strand at 63.316 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 60,473 - GFF78 0.53 +0.6 60,866 + GFF78 0.73 -0.1 61,110 - GFF78 0.84 +0.2 61,235 - -0.1 61,374 - -0.5 61,374 - +0.2 61,449 + -0.1 61,493 + -1.2 61,493 + +0.1 61,494 - +1.7 61,626 + GFF79 0.22 +0.3 61,627 - GFF79 0.23 +0.0 61,628 + GFF79 0.23 -0.4 61,629 - GFF79 0.23 +0.5 61,755 + GFF79 0.37 +0.1 61,756 - GFF79 0.37 -1.7 61,845 - GFF79 0.47 -0.1 61,845 - GFF79 0.47 +0.2 61,945 - GFF79 0.58 +0.1 62,123 + GFF79 0.78 +0.0 62,124 - GFF79 0.78 -1.1 62,281 + +0.3 62,295 + +0.2 62,304 - -0.2 62,317 + +0.9 62,318 - -0.1 62,318 - +0.1 62,320 + +0.6 62,320 + +0.4 62,381 - -0.1 62,464 + GFF80 0.19 -0.2 62,704 - GFF80 0.49 +0.0 62,730 + GFF80 0.52 +0.4 62,848 + GFF80 0.67 +0.1 62,966 + GFF80 0.81 +1.6 62,987 + GFF80 0.84 +0.8 62,988 - GFF80 0.84 +0.3 63,087 - -1.5 63,220 + +0.3 63,263 + +0.1 63,316 + +0.7 63,316 + -0.3
Or see this region's nucleotide sequence