Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF68

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF66 and GFF67 overlap by 1 nucleotidesGFF67 and GFF68 are separated by 26 nucleotidesGFF68 and GFF69 are separated by 107 nucleotidesGFF69 and GFF70 overlap by 1 nucleotides GFF66 - H(+)/Cl(-) exchange transporter ClcA, at 49,007 to 50,428 GFF66 GFF67 - hypothetical protein, at 50,428 to 50,571 GFF67 GFF68 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 50,598 to 51,878 GFF68 GFF69 - Uncharacterized protein YadU in stf fimbrial cluster, at 51,986 to 52,825 GFF69 GFF70 - Minor fimbrial subunit StfG, at 52,825 to 53,355 GFF70 Position (kb) 50 51 52Strain fitness (log2 ratio) -2 -1 0 1at 49.666 kb on - strand, within GFF66at 49.736 kb on + strand, within GFF66at 49.829 kb on - strand, within GFF66at 49.997 kb on + strand, within GFF66at 50.166 kb on - strand, within GFF66at 50.294 kb on - strandat 50.448 kb on - strand, within GFF67at 50.451 kb on - strand, within GFF67at 50.553 kb on + strand, within GFF67at 51.902 kb on - strandat 52.001 kb on - strandat 52.003 kb on + strandat 52.003 kb on + strandat 52.005 kb on + strandat 52.005 kb on + strandat 52.069 kb on + strandat 52.156 kb on + strand, within GFF69at 52.160 kb on - strand, within GFF69at 52.239 kb on + strand, within GFF69at 52.239 kb on + strand, within GFF69at 52.239 kb on + strand, within GFF69at 52.240 kb on - strand, within GFF69at 52.244 kb on - strand, within GFF69at 52.247 kb on + strand, within GFF69at 52.248 kb on - strand, within GFF69at 52.248 kb on - strand, within GFF69at 52.327 kb on + strand, within GFF69at 52.449 kb on + strand, within GFF69at 52.449 kb on + strand, within GFF69at 52.449 kb on + strand, within GFF69at 52.559 kb on + strand, within GFF69at 52.560 kb on - strand, within GFF69at 52.560 kb on - strand, within GFF69at 52.648 kb on - strand, within GFF69at 52.648 kb on - strand, within GFF69at 52.650 kb on + strand, within GFF69at 52.651 kb on - strand, within GFF69at 52.714 kb on + strand, within GFF69at 52.719 kb on + strand, within GFF69at 52.720 kb on - strand, within GFF69at 52.720 kb on - strand, within GFF69at 52.723 kb on - strand, within GFF69at 52.828 kb on + strandat 52.828 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
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49,666 - GFF66 0.46 +0.1
49,736 + GFF66 0.51 +0.5
49,829 - GFF66 0.58 -0.1
49,997 + GFF66 0.70 +0.1
50,166 - GFF66 0.82 +0.0
50,294 - -0.2
50,448 - GFF67 0.14 +0.7
50,451 - GFF67 0.16 +0.3
50,553 + GFF67 0.87 +0.9
51,902 - +0.3
52,001 - +1.0
52,003 + -0.7
52,003 + +0.3
52,005 + -0.8
52,005 + +0.0
52,069 + +0.1
52,156 + GFF69 0.20 +0.3
52,160 - GFF69 0.21 -1.9
52,239 + GFF69 0.30 +0.2
52,239 + GFF69 0.30 +0.3
52,239 + GFF69 0.30 +0.2
52,240 - GFF69 0.30 +0.5
52,244 - GFF69 0.31 -0.5
52,247 + GFF69 0.31 +1.1
52,248 - GFF69 0.31 -0.4
52,248 - GFF69 0.31 -0.1
52,327 + GFF69 0.41 +0.3
52,449 + GFF69 0.55 +0.1
52,449 + GFF69 0.55 +0.2
52,449 + GFF69 0.55 -0.4
52,559 + GFF69 0.68 +0.4
52,560 - GFF69 0.68 +0.8
52,560 - GFF69 0.68 -0.2
52,648 - GFF69 0.79 +0.3
52,648 - GFF69 0.79 +0.3
52,650 + GFF69 0.79 +0.9
52,651 - GFF69 0.79 +0.0
52,714 + GFF69 0.87 +0.7
52,719 + GFF69 0.87 -0.8
52,720 - GFF69 0.87 +0.2
52,720 - GFF69 0.87 -0.3
52,723 - GFF69 0.88 +0.9
52,828 + +0.2
52,828 + -0.5

Or see this region's nucleotide sequence