Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4758 and GFF4759 overlap by 1 nucleotides GFF4759 and GFF4760 overlap by 23 nucleotides GFF4760 and GFF4761 overlap by 4 nucleotides GFF4761 and GFF4762 are separated by 23 nucleotides
GFF4758 - Type III secretion host injection and negative regulator protein (YopD), at 65,029 to 66,039
GFF4758
GFF4759 - Surface presentation of antigens protein SpaM, at 66,039 to 66,482
GFF4759
GFF4760 - Type III secretion cytoplasmic ATP synthase (EC 3.6.3.14, YscN,SpaL,MxiB,HrcN,EscN), at 66,460 to 67,755
GFF4760
GFF4761 - Type III secretion system protein BsaR, at 67,752 to 68,159
GFF4761
GFF4762 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 68,183 to 70,240
GFF4762
Position (kb) 66
67
68 Strain fitness (log2 ratio) -1
0
1 at 65.526 kb on + strand, within GFF4758 at 65.551 kb on - strand, within GFF4758 at 65.585 kb on - strand, within GFF4758 at 65.639 kb on - strand, within GFF4758 at 66.092 kb on - strand, within GFF4759 at 66.268 kb on - strand, within GFF4759 at 66.456 kb on + strand at 66.457 kb on - strand at 66.716 kb on - strand, within GFF4760 at 66.777 kb on - strand, within GFF4760 at 66.855 kb on - strand, within GFF4760 at 67.049 kb on + strand, within GFF4760 at 67.161 kb on + strand, within GFF4760 at 67.162 kb on - strand, within GFF4760 at 67.319 kb on - strand, within GFF4760 at 67.380 kb on - strand, within GFF4760 at 67.527 kb on - strand, within GFF4760 at 67.594 kb on + strand, within GFF4760 at 67.595 kb on - strand, within GFF4760 at 67.696 kb on - strand at 67.730 kb on + strand at 67.731 kb on - strand at 67.860 kb on - strand, within GFF4761 at 67.962 kb on + strand, within GFF4761 at 68.005 kb on - strand, within GFF4761 at 68.160 kb on - strand at 68.305 kb on + strand at 68.336 kb on + strand at 68.350 kb on + strand at 68.533 kb on + strand, within GFF4762 at 68.656 kb on + strand, within GFF4762
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 65,526 + GFF4758 0.49 +0.8 65,551 - GFF4758 0.52 -0.3 65,585 - GFF4758 0.55 +0.5 65,639 - GFF4758 0.60 +0.2 66,092 - GFF4759 0.12 -0.0 66,268 - GFF4759 0.52 +0.1 66,456 + +0.8 66,457 - +0.0 66,716 - GFF4760 0.20 -0.9 66,777 - GFF4760 0.24 -0.0 66,855 - GFF4760 0.30 -0.6 67,049 + GFF4760 0.45 -1.0 67,161 + GFF4760 0.54 -1.4 67,162 - GFF4760 0.54 -0.6 67,319 - GFF4760 0.66 -0.6 67,380 - GFF4760 0.71 +0.2 67,527 - GFF4760 0.82 +0.6 67,594 + GFF4760 0.88 +0.5 67,595 - GFF4760 0.88 -1.2 67,696 - +0.1 67,730 + -0.2 67,731 - -0.3 67,860 - GFF4761 0.26 +0.1 67,962 + GFF4761 0.51 +0.1 68,005 - GFF4761 0.62 +0.3 68,160 - +0.0 68,305 + +0.0 68,336 + -0.6 68,350 + +0.1 68,533 + GFF4762 0.17 -0.7 68,656 + GFF4762 0.23 +0.1
Or see this region's nucleotide sequence