Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4484 and GFF4485 are separated by 288 nucleotides GFF4485 and GFF4486 are separated by 5 nucleotides GFF4486 and GFF4487 are separated by 90 nucleotides
GFF4484 - Transcriptional regulator, TetR family, at 268,360 to 268,941
GFF4484
GFF4485 - Transcriptional activator RamA, at 269,230 to 269,571
GFF4485
GFF4486 - Putative inner membrane protein, at 269,577 to 269,801
GFF4486
GFF4487 - FIG00638355: hypothetical protein, at 269,892 to 271,010
GFF4487
Position (kb) 269
270 Strain fitness (log2 ratio) -1
0
1 at 268.668 kb on - strand, within GFF4484 at 268.767 kb on - strand, within GFF4484 at 269.040 kb on - strand at 269.101 kb on + strand at 269.157 kb on - strand at 269.166 kb on - strand at 269.281 kb on - strand, within GFF4485 at 269.298 kb on - strand, within GFF4485 at 269.311 kb on - strand, within GFF4485 at 269.358 kb on + strand, within GFF4485 at 269.365 kb on + strand, within GFF4485 at 269.366 kb on - strand, within GFF4485 at 269.408 kb on + strand, within GFF4485 at 269.409 kb on - strand, within GFF4485 at 269.457 kb on + strand, within GFF4485 at 269.457 kb on + strand, within GFF4485 at 269.884 kb on + strand at 269.884 kb on + strand at 269.885 kb on - strand at 269.934 kb on + strand at 269.972 kb on - strand at 270.080 kb on + strand, within GFF4487 at 270.081 kb on - strand, within GFF4487 at 270.081 kb on - strand, within GFF4487 at 270.112 kb on + strand, within GFF4487 at 270.171 kb on - strand, within GFF4487 at 270.255 kb on - strand, within GFF4487 at 270.303 kb on + strand, within GFF4487 at 270.373 kb on + strand, within GFF4487 at 270.501 kb on - strand, within GFF4487 at 270.502 kb on + strand, within GFF4487 at 270.527 kb on + strand, within GFF4487 at 270.634 kb on - strand, within GFF4487 at 270.634 kb on - strand, within GFF4487 at 270.637 kb on + strand, within GFF4487 at 270.674 kb on + strand, within GFF4487 at 270.777 kb on - strand, within GFF4487
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 268,668 - GFF4484 0.53 +0.1 268,767 - GFF4484 0.70 -0.2 269,040 - -0.2 269,101 + +0.5 269,157 - -0.2 269,166 - -0.3 269,281 - GFF4485 0.15 -0.1 269,298 - GFF4485 0.20 -0.2 269,311 - GFF4485 0.24 -0.5 269,358 + GFF4485 0.37 -0.1 269,365 + GFF4485 0.39 -1.2 269,366 - GFF4485 0.40 +0.2 269,408 + GFF4485 0.52 -0.5 269,409 - GFF4485 0.52 -0.1 269,457 + GFF4485 0.66 -0.0 269,457 + GFF4485 0.66 -0.8 269,884 + +0.0 269,884 + +0.2 269,885 - -0.1 269,934 + -0.6 269,972 - +0.2 270,080 + GFF4487 0.17 -0.2 270,081 - GFF4487 0.17 +0.1 270,081 - GFF4487 0.17 -0.4 270,112 + GFF4487 0.20 -1.3 270,171 - GFF4487 0.25 -1.2 270,255 - GFF4487 0.32 -0.2 270,303 + GFF4487 0.37 +0.0 270,373 + GFF4487 0.43 -0.4 270,501 - GFF4487 0.54 -0.1 270,502 + GFF4487 0.55 -0.2 270,527 + GFF4487 0.57 +0.0 270,634 - GFF4487 0.66 +0.1 270,634 - GFF4487 0.66 -0.5 270,637 + GFF4487 0.67 +0.3 270,674 + GFF4487 0.70 -0.2 270,777 - GFF4487 0.79 -0.4
Or see this region's nucleotide sequence