Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4133

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4131 and GFF4132 are separated by 129 nucleotidesGFF4132 and GFF4133 overlap by 4 nucleotidesGFF4133 and GFF4134 are separated by 23 nucleotidesGFF4134 and GFF4135 are separated by 73 nucleotides GFF4131 - PTS system, glucose-specific IIC component (EC 2.7.1.69) / PTS system, glucose-specific IIB component (EC 2.7.1.69) / PTS system, glucose-specific IIA component (EC 2.7.1.69), at 13,769 to 15,310 GFF4131 GFF4132 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 15,440 to 16,282 GFF4132 GFF4133 - 6-phospho-3-hexuloisomerase, at 16,279 to 16,845 GFF4133 GFF4134 - D-arabino-3-hexulose 6-phosphate formaldehyde lyase, at 16,869 to 17,504 GFF4134 GFF4135 - FIG01047465: hypothetical protein, at 17,578 to 18,780 GFF4135 Position (kb) 16 17Strain fitness (log2 ratio) -1 0 1at 15.359 kb on + strandat 15.363 kb on + strandat 15.363 kb on + strandat 15.364 kb on - strandat 15.367 kb on + strandat 15.367 kb on + strandat 15.368 kb on - strandat 15.399 kb on + strandat 15.400 kb on - strandat 15.416 kb on - strandat 15.416 kb on - strandat 15.424 kb on + strandat 15.425 kb on - strandat 15.426 kb on + strandat 15.427 kb on - strandat 15.427 kb on - strandat 15.603 kb on - strand, within GFF4132at 15.662 kb on + strand, within GFF4132at 15.776 kb on + strand, within GFF4132at 15.776 kb on + strand, within GFF4132at 15.783 kb on + strand, within GFF4132at 15.783 kb on + strand, within GFF4132at 15.787 kb on + strand, within GFF4132at 15.788 kb on - strand, within GFF4132at 15.803 kb on + strand, within GFF4132at 15.804 kb on - strand, within GFF4132at 15.804 kb on - strand, within GFF4132at 15.898 kb on + strand, within GFF4132at 15.899 kb on - strand, within GFF4132at 15.983 kb on - strand, within GFF4132at 15.993 kb on - strand, within GFF4132at 16.010 kb on + strand, within GFF4132at 16.144 kb on + strand, within GFF4132at 16.211 kb on + strandat 16.314 kb on + strandat 16.676 kb on - strand, within GFF4133at 16.717 kb on - strand, within GFF4133at 16.802 kb on - strandat 16.843 kb on + strandat 17.107 kb on - strand, within GFF4134at 17.119 kb on + strand, within GFF4134at 17.196 kb on - strand, within GFF4134at 17.255 kb on + strand, within GFF4134at 17.255 kb on + strand, within GFF4134at 17.255 kb on + strand, within GFF4134at 17.267 kb on - strand, within GFF4134at 17.394 kb on + strand, within GFF4134at 17.416 kb on + strand, within GFF4134at 17.417 kb on - strand, within GFF4134at 17.579 kb on + strandat 17.580 kb on - strandat 17.580 kb on - strandat 17.613 kb on - strandat 17.669 kb on - strandat 17.702 kb on - strand, within GFF4135

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
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15,359 + +0.2
15,363 + -0.4
15,363 + +0.2
15,364 - +0.5
15,367 + -0.1
15,367 + -0.2
15,368 - -0.4
15,399 + +0.1
15,400 - +0.2
15,416 - +0.5
15,416 - +0.1
15,424 + +0.8
15,425 - +0.3
15,426 + -0.2
15,427 - +0.8
15,427 - -0.5
15,603 - GFF4132 0.19 +0.1
15,662 + GFF4132 0.26 +0.3
15,776 + GFF4132 0.40 +0.0
15,776 + GFF4132 0.40 -0.7
15,783 + GFF4132 0.41 -0.0
15,783 + GFF4132 0.41 +1.4
15,787 + GFF4132 0.41 +0.4
15,788 - GFF4132 0.41 -0.1
15,803 + GFF4132 0.43 +0.6
15,804 - GFF4132 0.43 +0.3
15,804 - GFF4132 0.43 +0.8
15,898 + GFF4132 0.54 +0.2
15,899 - GFF4132 0.54 -0.6
15,983 - GFF4132 0.64 +0.7
15,993 - GFF4132 0.66 -0.0
16,010 + GFF4132 0.68 +0.1
16,144 + GFF4132 0.84 -0.2
16,211 + -0.1
16,314 + +0.7
16,676 - GFF4133 0.70 +0.0
16,717 - GFF4133 0.77 -0.6
16,802 - +1.4
16,843 + +0.1
17,107 - GFF4134 0.37 -0.8
17,119 + GFF4134 0.39 -0.0
17,196 - GFF4134 0.51 -0.2
17,255 + GFF4134 0.61 -1.6
17,255 + GFF4134 0.61 +0.5
17,255 + GFF4134 0.61 +0.2
17,267 - GFF4134 0.63 -0.4
17,394 + GFF4134 0.83 +0.2
17,416 + GFF4134 0.86 +0.2
17,417 - GFF4134 0.86 -0.1
17,579 + +0.0
17,580 - +0.5
17,580 - +0.1
17,613 - -1.0
17,669 - +0.4
17,702 - GFF4135 0.10 -0.5

Or see this region's nucleotide sequence