Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3405 and GFF3406 are separated by 102 nucleotides GFF3406 and GFF3407 are separated by 90 nucleotides GFF3407 and GFF3408 are separated by 58 nucleotides GFF3408 and GFF3409 are separated by 93 nucleotides
GFF3405 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 170,801 to 171,580
GFF3405
GFF3406 - FIG01047625: hypothetical protein, at 171,683 to 171,805
GFF3406
GFF3407 - SifA protein, at 171,896 to 172,906
GFF3407
GFF3408 - FIG01045299: hypothetical protein, at 172,965 to 173,141
GFF3408
GFF3409 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 173,235 to 174,098
GFF3409
Position (kb) 171
172
173 Strain fitness (log2 ratio) -2
-1
0
1 at 171.223 kb on + strand, within GFF3405 at 171.272 kb on + strand, within GFF3405 at 171.276 kb on - strand, within GFF3405 at 171.276 kb on - strand, within GFF3405 at 171.524 kb on - strand at 171.524 kb on - strand at 171.531 kb on + strand at 171.537 kb on + strand at 171.598 kb on - strand at 171.767 kb on + strand, within GFF3406 at 171.768 kb on - strand, within GFF3406 at 171.797 kb on - strand at 171.849 kb on - strand at 171.945 kb on - strand at 171.992 kb on + strand at 172.052 kb on - strand, within GFF3407 at 172.463 kb on - strand, within GFF3407 at 173.244 kb on + strand at 173.374 kb on - strand, within GFF3409 at 173.746 kb on + strand, within GFF3409
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 171,223 + GFF3405 0.54 +0.9 171,272 + GFF3405 0.60 +0.6 171,276 - GFF3405 0.61 -1.9 171,276 - GFF3405 0.61 -1.1 171,524 - +0.1 171,524 - -1.5 171,531 + -0.1 171,537 + -0.6 171,598 - +0.4 171,767 + GFF3406 0.68 +0.3 171,768 - GFF3406 0.69 +0.2 171,797 - -1.3 171,849 - -0.5 171,945 - -0.4 171,992 + +0.0 172,052 - GFF3407 0.15 +0.7 172,463 - GFF3407 0.56 +1.1 173,244 + -0.0 173,374 - GFF3409 0.16 +1.5 173,746 + GFF3409 0.59 +0.0
Or see this region's nucleotide sequence