Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2745 and GFF2746 are separated by 121 nucleotides GFF2746 and GFF2747 are separated by 358 nucleotides GFF2747 and GFF2748 are separated by 265 nucleotides
GFF2745 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 264,184 to 265,104
GFF2745
GFF2746 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 265,226 to 266,857
GFF2746
GFF2747 - hypothetical protein, at 267,216 to 267,356
GFF2747
GFF2748 - Putative membrane protein, at 267,622 to 268,269
GFF2748
Position (kb) 265
266
267 Strain fitness (log2 ratio) -1
0
1
2 at 264.242 kb on - strand at 264.242 kb on - strand at 264.278 kb on - strand, within GFF2745 at 264.402 kb on - strand, within GFF2745 at 264.532 kb on - strand, within GFF2745 at 264.549 kb on + strand, within GFF2745 at 264.718 kb on - strand, within GFF2745 at 264.948 kb on - strand, within GFF2745 at 265.131 kb on + strand at 265.330 kb on - strand at 265.330 kb on - strand at 265.357 kb on + strand at 265.457 kb on - strand, within GFF2746 at 265.457 kb on - strand, within GFF2746 at 265.520 kb on - strand, within GFF2746 at 265.522 kb on - strand, within GFF2746 at 265.760 kb on - strand, within GFF2746 at 265.868 kb on - strand, within GFF2746 at 266.039 kb on - strand, within GFF2746 at 266.045 kb on - strand, within GFF2746 at 266.105 kb on - strand, within GFF2746 at 266.113 kb on - strand, within GFF2746 at 266.207 kb on - strand, within GFF2746 at 266.249 kb on - strand, within GFF2746 at 266.337 kb on - strand, within GFF2746 at 266.337 kb on - strand, within GFF2746 at 266.407 kb on + strand, within GFF2746 at 266.636 kb on + strand, within GFF2746 at 266.728 kb on + strand at 266.729 kb on - strand at 266.729 kb on - strand at 266.851 kb on + strand at 266.881 kb on - strand at 266.881 kb on - strand at 266.907 kb on - strand at 266.943 kb on - strand at 266.970 kb on - strand at 267.026 kb on + strand at 267.071 kb on - strand at 267.415 kb on + strand at 267.551 kb on + strand at 267.561 kb on + strand at 267.589 kb on + strand at 267.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 264,242 - -0.7 264,242 - -0.1 264,278 - GFF2745 0.10 +0.5 264,402 - GFF2745 0.24 +0.2 264,532 - GFF2745 0.38 -1.4 264,549 + GFF2745 0.40 +0.3 264,718 - GFF2745 0.58 -0.2 264,948 - GFF2745 0.83 +2.0 265,131 + -0.8 265,330 - -0.0 265,330 - +0.4 265,357 + +1.2 265,457 - GFF2746 0.14 +0.5 265,457 - GFF2746 0.14 +0.1 265,520 - GFF2746 0.18 +0.4 265,522 - GFF2746 0.18 +0.3 265,760 - GFF2746 0.33 +0.6 265,868 - GFF2746 0.39 +0.6 266,039 - GFF2746 0.50 +0.3 266,045 - GFF2746 0.50 +0.5 266,105 - GFF2746 0.54 +0.9 266,113 - GFF2746 0.54 +0.8 266,207 - GFF2746 0.60 +0.1 266,249 - GFF2746 0.63 -0.2 266,337 - GFF2746 0.68 +0.3 266,337 - GFF2746 0.68 +0.6 266,407 + GFF2746 0.72 +0.2 266,636 + GFF2746 0.86 -0.7 266,728 + +0.7 266,729 - +0.8 266,729 - +0.6 266,851 + +0.6 266,881 - +0.2 266,881 - +0.4 266,907 - +0.1 266,943 - +0.2 266,970 - +0.5 267,026 + +0.2 267,071 - +0.5 267,415 + +0.1 267,551 + +0.1 267,561 + +0.1 267,589 + -0.9 267,624 - +0.7
Or see this region's nucleotide sequence