Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2297 and GFF2298 are separated by 7 nucleotides GFF2298 and GFF2299 are separated by 28 nucleotides GFF2299 and GFF2300 are separated by 43 nucleotides
GFF2297 - Autoinducer 2 (AI-2) ABC transport system, membrane channel protein LsrD, at 84,997 to 85,995
GFF2297
GFF2298 - Autoinducer 2 (AI-2) ABC transport system, periplasmic AI-2 binding protein LsrB, at 86,003 to 87,046
GFF2298
GFF2299 - Autoinducer 2 (AI-2) aldolase LsrF (EC 4.2.1.-), at 87,075 to 87,950
GFF2299
GFF2300 - Autoinducer 2 (AI-2) modifying protein LsrG, at 87,994 to 88,323
GFF2300
Position (kb) 86
87
88 Strain fitness (log2 ratio) -2
-1
0
1
2 at 85.043 kb on + strand at 85.298 kb on + strand, within GFF2297 at 85.323 kb on + strand, within GFF2297 at 85.407 kb on + strand, within GFF2297 at 85.557 kb on + strand, within GFF2297 at 85.636 kb on + strand, within GFF2297 at 85.636 kb on + strand, within GFF2297 at 85.780 kb on + strand, within GFF2297 at 86.378 kb on - strand, within GFF2298 at 86.402 kb on + strand, within GFF2298 at 86.402 kb on + strand, within GFF2298 at 86.403 kb on - strand, within GFF2298 at 86.403 kb on - strand, within GFF2298 at 86.583 kb on + strand, within GFF2298 at 86.583 kb on + strand, within GFF2298 at 86.603 kb on + strand, within GFF2298 at 86.729 kb on - strand, within GFF2298 at 86.772 kb on + strand, within GFF2298 at 86.773 kb on - strand, within GFF2298 at 86.945 kb on - strand at 87.288 kb on + strand, within GFF2299 at 87.349 kb on + strand, within GFF2299 at 87.510 kb on - strand, within GFF2299 at 87.539 kb on + strand, within GFF2299 at 87.542 kb on + strand, within GFF2299 at 87.611 kb on + strand, within GFF2299 at 87.687 kb on + strand, within GFF2299 at 87.688 kb on - strand, within GFF2299 at 87.713 kb on + strand, within GFF2299 at 87.823 kb on + strand, within GFF2299 at 87.827 kb on + strand, within GFF2299 at 87.827 kb on + strand, within GFF2299 at 87.836 kb on + strand, within GFF2299 at 87.836 kb on + strand, within GFF2299 at 87.837 kb on - strand, within GFF2299 at 87.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 85,043 + -1.1 85,298 + GFF2297 0.30 +0.3 85,323 + GFF2297 0.33 -0.5 85,407 + GFF2297 0.41 -0.1 85,557 + GFF2297 0.56 +0.3 85,636 + GFF2297 0.64 +0.6 85,636 + GFF2297 0.64 -0.6 85,780 + GFF2297 0.78 -0.4 86,378 - GFF2298 0.36 +0.4 86,402 + GFF2298 0.38 -1.0 86,402 + GFF2298 0.38 -1.1 86,403 - GFF2298 0.38 -0.3 86,403 - GFF2298 0.38 -0.1 86,583 + GFF2298 0.56 +0.1 86,583 + GFF2298 0.56 +0.4 86,603 + GFF2298 0.57 -0.3 86,729 - GFF2298 0.70 +0.7 86,772 + GFF2298 0.74 +0.2 86,773 - GFF2298 0.74 -1.2 86,945 - +0.6 87,288 + GFF2299 0.24 +0.2 87,349 + GFF2299 0.31 +0.6 87,510 - GFF2299 0.50 +0.5 87,539 + GFF2299 0.53 -0.2 87,542 + GFF2299 0.53 +1.2 87,611 + GFF2299 0.61 -1.9 87,687 + GFF2299 0.70 +0.1 87,688 - GFF2299 0.70 +2.1 87,713 + GFF2299 0.73 -0.1 87,823 + GFF2299 0.85 +0.1 87,827 + GFF2299 0.86 +0.3 87,827 + GFF2299 0.86 +0.0 87,836 + GFF2299 0.87 -0.7 87,836 + GFF2299 0.87 -0.3 87,837 - GFF2299 0.87 +0.3 87,880 + +0.2
Or see this region's nucleotide sequence