Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2295 and GFF2296 overlap by 7 nucleotides GFF2296 and GFF2297 overlap by 1 nucleotides
GFF2295 - Autoinducer 2 (AI-2) ABC transport system, fused AI2 transporter subunits and ATP-binding component, at 82,425 to 83,960
GFF2295
GFF2296 - Autoinducer 2 (AI-2) ABC transport system, membrane channel protein LsrC, at 83,954 to 84,997
GFF2296
GFF2297 - Autoinducer 2 (AI-2) ABC transport system, membrane channel protein LsrD, at 84,997 to 85,995
GFF2297
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-1
0
1 at 83.129 kb on + strand, within GFF2295 at 83.130 kb on - strand, within GFF2295 at 83.227 kb on + strand, within GFF2295 at 83.230 kb on + strand, within GFF2295 at 83.267 kb on + strand, within GFF2295 at 83.268 kb on - strand, within GFF2295 at 83.449 kb on - strand, within GFF2295 at 83.821 kb on + strand at 83.822 kb on - strand at 83.842 kb on + strand at 83.842 kb on + strand at 83.842 kb on + strand at 83.843 kb on - strand at 83.843 kb on - strand at 83.844 kb on + strand at 83.844 kb on + strand at 83.844 kb on + strand at 83.845 kb on - strand at 83.845 kb on - strand at 83.897 kb on + strand at 83.906 kb on + strand at 83.906 kb on + strand at 84.013 kb on - strand at 84.044 kb on + strand at 84.147 kb on + strand, within GFF2296 at 84.209 kb on + strand, within GFF2296 at 84.241 kb on - strand, within GFF2296 at 84.243 kb on + strand, within GFF2296 at 84.244 kb on - strand, within GFF2296 at 84.355 kb on - strand, within GFF2296 at 84.469 kb on + strand, within GFF2296 at 84.841 kb on - strand, within GFF2296 at 84.922 kb on + strand at 85.043 kb on + strand at 85.298 kb on + strand, within GFF2297 at 85.323 kb on + strand, within GFF2297 at 85.407 kb on + strand, within GFF2297 at 85.557 kb on + strand, within GFF2297 at 85.636 kb on + strand, within GFF2297 at 85.636 kb on + strand, within GFF2297 at 85.780 kb on + strand, within GFF2297
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 83,129 + GFF2295 0.46 -0.2 83,130 - GFF2295 0.46 +0.3 83,227 + GFF2295 0.52 +0.9 83,230 + GFF2295 0.52 +0.5 83,267 + GFF2295 0.55 +0.7 83,268 - GFF2295 0.55 +0.3 83,449 - GFF2295 0.67 -0.3 83,821 + +1.4 83,822 - +0.3 83,842 + +0.8 83,842 + -0.7 83,842 + +0.4 83,843 - +0.3 83,843 - +0.2 83,844 + -1.0 83,844 + -0.8 83,844 + -0.5 83,845 - -0.2 83,845 - +0.0 83,897 + -0.2 83,906 + +0.4 83,906 + +0.1 84,013 - -0.2 84,044 + +0.6 84,147 + GFF2296 0.18 +0.7 84,209 + GFF2296 0.24 +1.0 84,241 - GFF2296 0.27 +0.1 84,243 + GFF2296 0.28 +0.2 84,244 - GFF2296 0.28 -0.6 84,355 - GFF2296 0.38 +0.2 84,469 + GFF2296 0.49 +0.3 84,841 - GFF2296 0.85 -0.1 84,922 + -1.1 85,043 + -1.1 85,298 + GFF2297 0.30 +0.3 85,323 + GFF2297 0.33 -0.5 85,407 + GFF2297 0.41 -0.1 85,557 + GFF2297 0.56 +0.3 85,636 + GFF2297 0.64 +0.6 85,636 + GFF2297 0.64 -0.6 85,780 + GFF2297 0.78 -0.4
Or see this region's nucleotide sequence