Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2275 and GFF2276 are separated by 328 nucleotides GFF2276 and GFF2277 are separated by 69 nucleotides GFF2277 and GFF2278 are separated by 11 nucleotides
GFF2275 - Predicted rhamnogalacturonide-specific TRAP-type transporter, substrate-binding component RhiA, at 64,151 to 65,134
GFF2275
GFF2276 - Manganese superoxide dismutase (EC 1.15.1.1), at 65,463 to 66,083
GFF2276
GFF2277 - Uncharacterized protein conserved in bacteria, at 66,153 to 66,842
GFF2277
GFF2278 - Putative inner membrane protein, at 66,854 to 67,249
GFF2278
Position (kb) 65
66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 64.539 kb on - strand, within GFF2275 at 64.539 kb on - strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.549 kb on - strand, within GFF2275 at 64.646 kb on + strand, within GFF2275 at 64.682 kb on - strand, within GFF2275 at 64.715 kb on + strand, within GFF2275 at 64.749 kb on + strand, within GFF2275 at 64.877 kb on - strand, within GFF2275 at 64.951 kb on - strand, within GFF2275 at 64.951 kb on - strand, within GFF2275 at 64.955 kb on + strand, within GFF2275 at 64.956 kb on - strand, within GFF2275 at 65.094 kb on - strand at 65.115 kb on - strand at 65.115 kb on - strand at 65.117 kb on - strand at 65.402 kb on + strand at 65.462 kb on + strand at 65.469 kb on + strand at 65.471 kb on + strand at 65.472 kb on - strand at 65.565 kb on + strand, within GFF2276 at 65.566 kb on - strand, within GFF2276 at 65.985 kb on + strand, within GFF2276 at 65.986 kb on - strand, within GFF2276 at 66.176 kb on + strand at 66.177 kb on - strand at 66.322 kb on + strand, within GFF2277 at 66.323 kb on - strand, within GFF2277 at 66.449 kb on + strand, within GFF2277 at 66.462 kb on + strand, within GFF2277 at 66.522 kb on - strand, within GFF2277 at 66.692 kb on + strand, within GFF2277 at 66.692 kb on + strand, within GFF2277 at 66.693 kb on - strand, within GFF2277 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.939 kb on - strand, within GFF2278 at 66.939 kb on - strand, within GFF2278 at 66.940 kb on + strand, within GFF2278 at 67.001 kb on - strand, within GFF2278 at 67.080 kb on - strand, within GFF2278
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 64,539 - GFF2275 0.39 +0.3 64,539 - GFF2275 0.39 +0.2 64,546 + GFF2275 0.40 +0.2 64,546 + GFF2275 0.40 -0.1 64,547 - GFF2275 0.40 +0.4 64,547 - GFF2275 0.40 +0.1 64,548 + GFF2275 0.40 +0.1 64,548 + GFF2275 0.40 +0.7 64,549 - GFF2275 0.40 +0.3 64,646 + GFF2275 0.50 -0.3 64,682 - GFF2275 0.54 -0.5 64,715 + GFF2275 0.57 -0.1 64,749 + GFF2275 0.61 -0.1 64,877 - GFF2275 0.74 +0.1 64,951 - GFF2275 0.81 +0.0 64,951 - GFF2275 0.81 +0.6 64,955 + GFF2275 0.82 +0.4 64,956 - GFF2275 0.82 +0.5 65,094 - -0.8 65,115 - -0.0 65,115 - +0.3 65,117 - -0.1 65,402 + +0.2 65,462 + -1.2 65,469 + -1.5 65,471 + -1.9 65,472 - -1.2 65,565 + GFF2276 0.16 -0.8 65,566 - GFF2276 0.17 -0.6 65,985 + GFF2276 0.84 +1.2 65,986 - GFF2276 0.84 -0.3 66,176 + +0.7 66,177 - +0.1 66,322 + GFF2277 0.24 +0.7 66,323 - GFF2277 0.25 -0.1 66,449 + GFF2277 0.43 +0.1 66,462 + GFF2277 0.45 +0.5 66,522 - GFF2277 0.53 +0.5 66,692 + GFF2277 0.78 +0.2 66,692 + GFF2277 0.78 +0.4 66,693 - GFF2277 0.78 -1.3 66,938 + GFF2278 0.21 +1.0 66,938 + GFF2278 0.21 +0.2 66,938 + GFF2278 0.21 -0.4 66,938 + GFF2278 0.21 +2.1 66,939 - GFF2278 0.21 +0.2 66,939 - GFF2278 0.21 +0.3 66,940 + GFF2278 0.22 +0.2 67,001 - GFF2278 0.37 +0.3 67,080 - GFF2278 0.57 +0.4
Or see this region's nucleotide sequence