Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2249 and GFF2250 are separated by 46 nucleotides GFF2250 and GFF2251 overlap by 4 nucleotides GFF2251 and GFF2252 overlap by 4 nucleotides GFF2252 and GFF2253 are separated by 12 nucleotides
GFF2249 - Putative merR family bacterial regulatory protein, at 40,872 to 41,162
GFF2249
GFF2250 - formate dehydrogenase formation protein FdhE, at 41,209 to 42,138
GFF2250
GFF2251 - Formate dehydrogenase O gamma subunit (EC 1.2.1.2), at 42,135 to 42,770
GFF2251
GFF2252 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 42,767 to 43,669
GFF2252
GFF2253 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 43,682 to 46,732
GFF2253
Position (kb) 42
43 Strain fitness (log2 ratio) -1
0
1 at 41.143 kb on + strand at 41.144 kb on - strand at 41.144 kb on - strand at 41.181 kb on + strand at 41.357 kb on + strand, within GFF2250 at 41.357 kb on + strand, within GFF2250 at 41.425 kb on + strand, within GFF2250 at 41.426 kb on - strand, within GFF2250 at 41.494 kb on - strand, within GFF2250 at 41.573 kb on + strand, within GFF2250 at 41.662 kb on - strand, within GFF2250 at 41.798 kb on - strand, within GFF2250 at 41.838 kb on + strand, within GFF2250 at 41.899 kb on - strand, within GFF2250 at 41.933 kb on + strand, within GFF2250 at 42.038 kb on + strand, within GFF2250 at 42.039 kb on - strand, within GFF2250 at 42.125 kb on - strand at 42.236 kb on + strand, within GFF2251 at 42.269 kb on + strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.291 kb on + strand, within GFF2251 at 42.291 kb on + strand, within GFF2251 at 42.381 kb on + strand, within GFF2251 at 42.582 kb on - strand, within GFF2251 at 42.740 kb on + strand at 42.740 kb on + strand at 42.742 kb on + strand at 42.844 kb on - strand at 42.993 kb on + strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 43.061 kb on + strand, within GFF2252 at 43.189 kb on + strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.195 kb on + strand, within GFF2252 at 43.252 kb on + strand, within GFF2252 at 43.253 kb on - strand, within GFF2252 at 43.270 kb on + strand, within GFF2252 at 43.369 kb on + strand, within GFF2252 at 43.370 kb on - strand, within GFF2252 at 43.454 kb on + strand, within GFF2252 at 43.474 kb on - strand, within GFF2252 at 43.670 kb on - strand at 43.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 41,143 + +0.7 41,144 - +0.2 41,144 - +0.1 41,181 + -0.6 41,357 + GFF2250 0.16 -0.1 41,357 + GFF2250 0.16 +0.6 41,425 + GFF2250 0.23 +1.2 41,426 - GFF2250 0.23 -0.5 41,494 - GFF2250 0.31 -0.1 41,573 + GFF2250 0.39 -0.0 41,662 - GFF2250 0.49 +0.2 41,798 - GFF2250 0.63 +0.0 41,838 + GFF2250 0.68 +0.4 41,899 - GFF2250 0.74 +0.3 41,933 + GFF2250 0.78 -0.2 42,038 + GFF2250 0.89 +0.3 42,039 - GFF2250 0.89 +0.0 42,125 - +0.1 42,236 + GFF2251 0.16 -0.9 42,269 + GFF2251 0.21 +0.4 42,270 - GFF2251 0.21 -0.2 42,270 - GFF2251 0.21 -0.1 42,270 - GFF2251 0.21 -0.2 42,270 - GFF2251 0.21 -0.1 42,291 + GFF2251 0.25 -0.7 42,291 + GFF2251 0.25 +0.1 42,381 + GFF2251 0.39 -0.1 42,582 - GFF2251 0.70 -0.6 42,740 + -0.2 42,740 + -0.1 42,742 + +0.3 42,844 - +0.5 42,993 + GFF2252 0.25 -0.4 42,994 - GFF2252 0.25 +0.3 42,994 - GFF2252 0.25 -0.0 42,994 - GFF2252 0.25 +0.5 43,061 + GFF2252 0.33 +0.7 43,189 + GFF2252 0.47 -0.2 43,190 - GFF2252 0.47 +0.7 43,190 - GFF2252 0.47 +0.2 43,190 - GFF2252 0.47 +0.6 43,195 + GFF2252 0.47 +0.1 43,252 + GFF2252 0.54 +0.4 43,253 - GFF2252 0.54 -0.6 43,270 + GFF2252 0.56 -0.7 43,369 + GFF2252 0.67 -0.4 43,370 - GFF2252 0.67 -0.1 43,454 + GFF2252 0.76 -0.8 43,474 - GFF2252 0.78 -0.2 43,670 - +0.3 43,670 - -0.4
Or see this region's nucleotide sequence