Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1789 and GFF1790 are separated by 99 nucleotides GFF1790 and GFF1791 are separated by 39 nucleotides GFF1791 and GFF1792 overlap by 14 nucleotides GFF1792 and GFF1793 are separated by 111 nucleotides GFF1793 and GFF1794 are separated by 168 nucleotides
GFF1789 - hypothetical protein, at 11,775 to 11,894
GFF1789
GFF1790 - FIG00634494: possible peptidase, at 11,994 to 12,815
GFF1790
GFF1791 - Spermidine export protein MdtI, at 12,855 to 13,184
GFF1791
GFF1792 - Spermidine export protein MdtJ, at 13,171 to 13,533
GFF1792
GFF1793 - FIG01046102: hypothetical protein, at 13,645 to 13,782
GFF1793
GFF1794 - Putative transport protein, at 13,951 to 14,985
GFF1794
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1 at 11.999 kb on + strand at 12.000 kb on - strand at 12.026 kb on - strand at 12.110 kb on + strand, within GFF1790 at 12.175 kb on + strand, within GFF1790 at 12.254 kb on - strand, within GFF1790 at 12.481 kb on + strand, within GFF1790 at 12.677 kb on - strand, within GFF1790 at 12.713 kb on - strand, within GFF1790 at 13.017 kb on + strand, within GFF1791 at 13.018 kb on - strand, within GFF1791 at 13.066 kb on - strand, within GFF1791 at 13.082 kb on + strand, within GFF1791 at 13.083 kb on - strand, within GFF1791 at 13.164 kb on + strand at 13.237 kb on + strand, within GFF1792 at 13.401 kb on + strand, within GFF1792 at 13.656 kb on + strand at 13.996 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 11,999 + -0.2 12,000 - -0.2 12,026 - -0.2 12,110 + GFF1790 0.14 +0.1 12,175 + GFF1790 0.22 +0.4 12,254 - GFF1790 0.32 -0.0 12,481 + GFF1790 0.59 +0.6 12,677 - GFF1790 0.83 -0.0 12,713 - GFF1790 0.87 -0.1 13,017 + GFF1791 0.49 -0.5 13,018 - GFF1791 0.49 -0.0 13,066 - GFF1791 0.64 +0.1 13,082 + GFF1791 0.69 -1.0 13,083 - GFF1791 0.69 -0.2 13,164 + +0.7 13,237 + GFF1792 0.18 -0.5 13,401 + GFF1792 0.63 +0.2 13,656 + -0.2 13,996 - -0.0
Or see this region's nucleotide sequence