Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1729

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1728 and GFF1729 are separated by 319 nucleotidesGFF1729 and GFF1730 are separated by 188 nucleotides GFF1728 - Putative membrane protein, at 26,398 to 27,096 GFF1728 GFF1729 - N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157), at 27,416 to 28,786 GFF1729 GFF1730 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 28,975 to 30,804 GFF1730 Position (kb) 27 28 29Strain fitness (log2 ratio) -1 0 1 2at 26.452 kb on - strandat 26.510 kb on + strand, within GFF1728at 26.511 kb on - strand, within GFF1728at 26.581 kb on + strand, within GFF1728at 26.581 kb on + strand, within GFF1728at 26.583 kb on + strand, within GFF1728at 26.583 kb on + strand, within GFF1728at 26.584 kb on - strand, within GFF1728at 26.689 kb on + strand, within GFF1728at 26.690 kb on - strand, within GFF1728at 26.741 kb on - strand, within GFF1728at 26.741 kb on - strand, within GFF1728at 26.742 kb on + strand, within GFF1728at 26.742 kb on + strand, within GFF1728at 26.743 kb on - strand, within GFF1728at 26.743 kb on - strand, within GFF1728at 26.860 kb on - strand, within GFF1728at 26.939 kb on + strand, within GFF1728at 26.983 kb on - strand, within GFF1728at 26.988 kb on + strand, within GFF1728at 27.172 kb on + strandat 27.222 kb on + strandat 27.415 kb on + strandat 28.846 kb on - strandat 28.949 kb on + strandat 28.949 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
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26,452 - +0.5
26,510 + GFF1728 0.16 +0.1
26,511 - GFF1728 0.16 +0.5
26,581 + GFF1728 0.26 +0.1
26,581 + GFF1728 0.26 +0.2
26,583 + GFF1728 0.26 +0.2
26,583 + GFF1728 0.26 +0.5
26,584 - GFF1728 0.27 +0.2
26,689 + GFF1728 0.42 +0.0
26,690 - GFF1728 0.42 -0.8
26,741 - GFF1728 0.49 +0.4
26,741 - GFF1728 0.49 +1.2
26,742 + GFF1728 0.49 -0.0
26,742 + GFF1728 0.49 +0.2
26,743 - GFF1728 0.49 +0.6
26,743 - GFF1728 0.49 +0.4
26,860 - GFF1728 0.66 -0.1
26,939 + GFF1728 0.77 +0.2
26,983 - GFF1728 0.84 -0.2
26,988 + GFF1728 0.84 +0.5
27,172 + +0.6
27,222 + -0.0
27,415 + +1.0
28,846 - +2.1
28,949 + +0.3
28,949 + -0.0

Or see this region's nucleotide sequence