Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1726 and GFF1727 are separated by 26 nucleotides GFF1727 and GFF1728 are separated by 229 nucleotides GFF1728 and GFF1729 are separated by 319 nucleotides
GFF1726 - ATP synthase beta chain (EC 3.6.3.14), at 24,346 to 25,728
GFF1726
GFF1727 - ATP synthase epsilon chain (EC 3.6.3.14), at 25,755 to 26,168
GFF1727
GFF1728 - Putative membrane protein, at 26,398 to 27,096
GFF1728
GFF1729 - N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157), at 27,416 to 28,786
GFF1729
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 26.066 kb on + strand, within GFF1727 at 26.306 kb on + strand at 26.372 kb on - strand at 26.379 kb on - strand at 26.391 kb on + strand at 26.392 kb on - strand at 26.395 kb on - strand at 26.395 kb on - strand at 26.395 kb on - strand at 26.452 kb on - strand at 26.510 kb on + strand, within GFF1728 at 26.511 kb on - strand, within GFF1728 at 26.581 kb on + strand, within GFF1728 at 26.581 kb on + strand, within GFF1728 at 26.583 kb on + strand, within GFF1728 at 26.583 kb on + strand, within GFF1728 at 26.584 kb on - strand, within GFF1728 at 26.689 kb on + strand, within GFF1728 at 26.690 kb on - strand, within GFF1728 at 26.741 kb on - strand, within GFF1728 at 26.741 kb on - strand, within GFF1728 at 26.742 kb on + strand, within GFF1728 at 26.742 kb on + strand, within GFF1728 at 26.743 kb on - strand, within GFF1728 at 26.743 kb on - strand, within GFF1728 at 26.860 kb on - strand, within GFF1728 at 26.939 kb on + strand, within GFF1728 at 26.983 kb on - strand, within GFF1728 at 26.988 kb on + strand, within GFF1728 at 27.172 kb on + strand at 27.222 kb on + strand at 27.415 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 26,066 + GFF1727 0.75 -2.8 26,306 + +0.4 26,372 - +1.0 26,379 - +0.1 26,391 + -0.7 26,392 - +0.4 26,395 - +0.4 26,395 - -0.5 26,395 - +0.7 26,452 - +0.5 26,510 + GFF1728 0.16 +0.1 26,511 - GFF1728 0.16 +0.5 26,581 + GFF1728 0.26 +0.1 26,581 + GFF1728 0.26 +0.2 26,583 + GFF1728 0.26 +0.2 26,583 + GFF1728 0.26 +0.5 26,584 - GFF1728 0.27 +0.2 26,689 + GFF1728 0.42 +0.0 26,690 - GFF1728 0.42 -0.8 26,741 - GFF1728 0.49 +0.4 26,741 - GFF1728 0.49 +1.2 26,742 + GFF1728 0.49 -0.0 26,742 + GFF1728 0.49 +0.2 26,743 - GFF1728 0.49 +0.6 26,743 - GFF1728 0.49 +0.4 26,860 - GFF1728 0.66 -0.1 26,939 + GFF1728 0.77 +0.2 26,983 - GFF1728 0.84 -0.2 26,988 + GFF1728 0.84 +0.5 27,172 + +0.6 27,222 + -0.0 27,415 + +1.0
Or see this region's nucleotide sequence