Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1671 and GFF1672 are separated by 124 nucleotides GFF1672 and GFF1673 are separated by 2 nucleotides GFF1673 and GFF1674 are separated by 795 nucleotides
GFF1671 - Flagellar motor rotation protein MotA, at 56,545 to 57,432
GFF1671
GFF1672 - Flagellar transcriptional activator FlhC, at 57,557 to 58,135
GFF1672
GFF1673 - Flagellar transcriptional activator FlhD, at 58,138 to 58,479
GFF1673
GFF1674 - Universal stress protein C, at 59,275 to 59,703
GFF1674
Position (kb) 58
59 Strain fitness (log2 ratio) -1
0
1 at 57.210 kb on - strand, within GFF1671 at 57.210 kb on - strand, within GFF1671 at 57.213 kb on + strand, within GFF1671 at 57.214 kb on - strand, within GFF1671 at 57.347 kb on + strand at 57.426 kb on + strand at 57.427 kb on - strand at 57.457 kb on - strand at 57.635 kb on + strand, within GFF1672 at 57.635 kb on + strand, within GFF1672 at 57.725 kb on - strand, within GFF1672 at 57.831 kb on + strand, within GFF1672 at 57.944 kb on - strand, within GFF1672 at 58.136 kb on + strand at 58.136 kb on + strand at 58.200 kb on + strand, within GFF1673 at 58.219 kb on + strand, within GFF1673 at 58.448 kb on + strand at 58.460 kb on - strand at 58.460 kb on - strand at 58.473 kb on + strand at 58.614 kb on + strand at 58.615 kb on - strand at 58.645 kb on - strand at 58.722 kb on + strand at 58.723 kb on - strand at 58.723 kb on - strand at 58.815 kb on - strand at 59.281 kb on + strand at 59.282 kb on - strand at 59.385 kb on + strand, within GFF1674 at 59.410 kb on + strand, within GFF1674 at 59.411 kb on - strand, within GFF1674
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 57,210 - GFF1671 0.75 +0.3 57,210 - GFF1671 0.75 +0.0 57,213 + GFF1671 0.75 +0.2 57,214 - GFF1671 0.75 -0.2 57,347 + -0.2 57,426 + +0.6 57,427 - +0.1 57,457 - -0.0 57,635 + GFF1672 0.13 +0.0 57,635 + GFF1672 0.13 -0.0 57,725 - GFF1672 0.29 -0.1 57,831 + GFF1672 0.47 +0.4 57,944 - GFF1672 0.67 +0.2 58,136 + +0.2 58,136 + +0.4 58,200 + GFF1673 0.18 +1.2 58,219 + GFF1673 0.24 +0.6 58,448 + -0.0 58,460 - +0.7 58,460 - +0.4 58,473 + +0.3 58,614 + -0.2 58,615 - +0.1 58,645 - -1.2 58,722 + +0.2 58,723 - +0.4 58,723 - +0.8 58,815 - -0.2 59,281 + -0.1 59,282 - -0.0 59,385 + GFF1674 0.26 +0.2 59,410 + GFF1674 0.31 +0.2 59,411 - GFF1674 0.32 +0.6
Or see this region's nucleotide sequence