Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1567

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1566 and GFF1567 are separated by 14 nucleotidesGFF1567 and GFF1568 are separated by 129 nucleotides GFF1566 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 28,316 to 29,764 GFF1566 GFF1567 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 29,779 to 31,062 GFF1567 GFF1568 - gamma-aminobutyrate (GABA) permease, at 31,192 to 32,592 GFF1568 Position (kb) 29 30 31 32Strain fitness (log2 ratio) -3 -2 -1 0 1at 28.933 kb on + strand, within GFF1566at 28.934 kb on - strand, within GFF1566at 29.194 kb on + strand, within GFF1566at 29.194 kb on + strand, within GFF1566at 29.195 kb on - strand, within GFF1566at 29.195 kb on - strand, within GFF1566at 29.201 kb on + strand, within GFF1566at 29.565 kb on - strand, within GFF1566at 30.026 kb on - strand, within GFF1567at 30.208 kb on + strand, within GFF1567at 30.209 kb on - strand, within GFF1567at 30.209 kb on - strand, within GFF1567at 30.277 kb on + strand, within GFF1567at 30.461 kb on + strand, within GFF1567at 30.481 kb on - strand, within GFF1567at 30.564 kb on + strand, within GFF1567at 30.565 kb on - strand, within GFF1567at 30.753 kb on + strand, within GFF1567at 30.955 kb on + strandat 30.959 kb on - strandat 31.060 kb on + strandat 31.061 kb on - strandat 31.061 kb on - strandat 31.092 kb on + strandat 31.093 kb on - strandat 31.093 kb on - strandat 31.140 kb on - strandat 31.178 kb on - strandat 31.188 kb on + strandat 31.342 kb on + strand, within GFF1568at 31.432 kb on + strand, within GFF1568at 31.467 kb on + strand, within GFF1568at 31.482 kb on - strand, within GFF1568at 31.483 kb on + strand, within GFF1568at 31.484 kb on - strand, within GFF1568at 31.574 kb on + strand, within GFF1568at 31.591 kb on - strand, within GFF1568at 31.616 kb on + strand, within GFF1568at 31.633 kb on + strand, within GFF1568at 31.665 kb on + strand, within GFF1568at 31.666 kb on - strand, within GFF1568at 31.666 kb on - strand, within GFF1568at 31.666 kb on - strand, within GFF1568at 31.729 kb on + strand, within GFF1568at 31.833 kb on + strand, within GFF1568at 31.852 kb on - strand, within GFF1568at 31.854 kb on + strand, within GFF1568at 31.854 kb on + strand, within GFF1568at 31.854 kb on + strand, within GFF1568at 31.855 kb on - strand, within GFF1568at 31.891 kb on - strand, within GFF1568at 31.891 kb on - strand, within GFF1568at 31.915 kb on - strand, within GFF1568at 31.936 kb on + strand, within GFF1568

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
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28,933 + GFF1566 0.43 -1.4
28,934 - GFF1566 0.43 +0.3
29,194 + GFF1566 0.61 -0.0
29,194 + GFF1566 0.61 +0.1
29,195 - GFF1566 0.61 +1.3
29,195 - GFF1566 0.61 -0.6
29,201 + GFF1566 0.61 +0.1
29,565 - GFF1566 0.86 -0.1
30,026 - GFF1567 0.19 -0.3
30,208 + GFF1567 0.33 -2.9
30,209 - GFF1567 0.33 +0.3
30,209 - GFF1567 0.33 -0.2
30,277 + GFF1567 0.39 -0.5
30,461 + GFF1567 0.53 +0.4
30,481 - GFF1567 0.55 -1.0
30,564 + GFF1567 0.61 +0.2
30,565 - GFF1567 0.61 -0.4
30,753 + GFF1567 0.76 -0.4
30,955 + -0.3
30,959 - +0.3
31,060 + -0.1
31,061 - +0.4
31,061 - -0.4
31,092 + +0.0
31,093 - +0.1
31,093 - -0.9
31,140 - +0.6
31,178 - +0.8
31,188 + -0.8
31,342 + GFF1568 0.11 +0.5
31,432 + GFF1568 0.17 -0.4
31,467 + GFF1568 0.20 -0.1
31,482 - GFF1568 0.21 +0.6
31,483 + GFF1568 0.21 -0.0
31,484 - GFF1568 0.21 -0.4
31,574 + GFF1568 0.27 +0.2
31,591 - GFF1568 0.28 -0.6
31,616 + GFF1568 0.30 -0.7
31,633 + GFF1568 0.31 -0.5
31,665 + GFF1568 0.34 -0.2
31,666 - GFF1568 0.34 -0.2
31,666 - GFF1568 0.34 -0.3
31,666 - GFF1568 0.34 -0.2
31,729 + GFF1568 0.38 +0.3
31,833 + GFF1568 0.46 +0.8
31,852 - GFF1568 0.47 +0.3
31,854 + GFF1568 0.47 +0.1
31,854 + GFF1568 0.47 +0.1
31,854 + GFF1568 0.47 -1.6
31,855 - GFF1568 0.47 +0.6
31,891 - GFF1568 0.50 -0.0
31,891 - GFF1568 0.50 +0.4
31,915 - GFF1568 0.52 -0.3
31,936 + GFF1568 0.53 -0.0

Or see this region's nucleotide sequence