Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1521 and GFF1522 are separated by 157 nucleotides GFF1522 and GFF1523 are separated by 110 nucleotides
GFF1521 - rRNA small subunit methyltransferase I, at 25,295 to 26,158
GFF1521
GFF1522 - Galactitol utilization operon repressor, at 26,316 to 27,089
GFF1522
GFF1523 - Galactitol-1-phosphate 5-dehydrogenase (EC 1.1.1.251), at 27,200 to 28,243
GFF1523
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2 at 25.615 kb on + strand, within GFF1521 at 25.615 kb on + strand, within GFF1521 at 25.616 kb on - strand, within GFF1521 at 25.822 kb on - strand, within GFF1521 at 25.865 kb on + strand, within GFF1521 at 26.127 kb on - strand at 26.129 kb on - strand at 26.202 kb on - strand at 26.461 kb on - strand, within GFF1522 at 26.529 kb on - strand, within GFF1522 at 26.676 kb on - strand, within GFF1522 at 26.691 kb on - strand, within GFF1522 at 26.691 kb on - strand, within GFF1522 at 26.712 kb on - strand, within GFF1522 at 26.724 kb on - strand, within GFF1522 at 26.724 kb on - strand, within GFF1522 at 26.727 kb on - strand, within GFF1522 at 26.761 kb on - strand, within GFF1522 at 27.009 kb on - strand, within GFF1522 at 27.047 kb on + strand at 27.048 kb on - strand at 27.048 kb on - strand at 27.197 kb on - strand at 27.375 kb on + strand, within GFF1523 at 27.431 kb on - strand, within GFF1523 at 27.459 kb on - strand, within GFF1523 at 27.459 kb on - strand, within GFF1523 at 27.620 kb on - strand, within GFF1523 at 27.749 kb on - strand, within GFF1523 at 27.753 kb on - strand, within GFF1523 at 27.795 kb on - strand, within GFF1523 at 27.795 kb on - strand, within GFF1523 at 27.917 kb on + strand, within GFF1523 at 27.917 kb on + strand, within GFF1523 at 27.928 kb on - strand, within GFF1523 at 28.029 kb on - strand, within GFF1523 at 28.073 kb on + strand, within GFF1523 at 28.079 kb on - strand, within GFF1523 at 28.082 kb on - strand, within GFF1523 at 28.087 kb on - strand, within GFF1523 at 28.089 kb on + strand, within GFF1523
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 25,615 + GFF1521 0.37 +0.3 25,615 + GFF1521 0.37 -0.5 25,616 - GFF1521 0.37 +0.3 25,822 - GFF1521 0.61 +0.1 25,865 + GFF1521 0.66 -0.3 26,127 - +0.3 26,129 - +1.1 26,202 - -0.5 26,461 - GFF1522 0.19 -0.3 26,529 - GFF1522 0.28 +0.1 26,676 - GFF1522 0.47 +0.4 26,691 - GFF1522 0.48 -0.7 26,691 - GFF1522 0.48 -1.3 26,712 - GFF1522 0.51 -2.1 26,724 - GFF1522 0.53 +0.3 26,724 - GFF1522 0.53 -1.1 26,727 - GFF1522 0.53 +0.1 26,761 - GFF1522 0.57 -0.6 27,009 - GFF1522 0.90 -0.4 27,047 + -0.7 27,048 - +1.0 27,048 - -0.0 27,197 - -0.3 27,375 + GFF1523 0.17 +0.0 27,431 - GFF1523 0.22 +2.2 27,459 - GFF1523 0.25 +2.1 27,459 - GFF1523 0.25 +0.5 27,620 - GFF1523 0.40 +0.1 27,749 - GFF1523 0.53 +0.7 27,753 - GFF1523 0.53 -0.9 27,795 - GFF1523 0.57 -0.0 27,795 - GFF1523 0.57 +0.0 27,917 + GFF1523 0.69 -0.4 27,917 + GFF1523 0.69 -0.4 27,928 - GFF1523 0.70 -0.4 28,029 - GFF1523 0.79 +1.0 28,073 + GFF1523 0.84 +0.0 28,079 - GFF1523 0.84 +1.5 28,082 - GFF1523 0.84 +0.0 28,087 - GFF1523 0.85 +0.6 28,089 + GFF1523 0.85 -0.7
Or see this region's nucleotide sequence