Experiment: LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1324 and GFF1325 overlap by 29 nucleotides GFF1325 and GFF1326 are separated by 112 nucleotides
GFF1324 - Transcriptional activator protein LysR, at 32,883 to 33,818
GFF1324
GFF1325 - Aspartate racemase (EC 5.1.1.13), at 33,790 to 34,497
GFF1325
GFF1326 - Arabinose-proton symporter, at 34,610 to 36,028
GFF1326
Position (kb) 33
34
35 Strain fitness (log2 ratio) -2
-1
0
1 at 32.883 kb on - strand at 33.055 kb on + strand, within GFF1324 at 33.094 kb on + strand, within GFF1324 at 33.220 kb on + strand, within GFF1324 at 33.261 kb on - strand, within GFF1324 at 33.370 kb on + strand, within GFF1324 at 33.371 kb on - strand, within GFF1324 at 33.394 kb on + strand, within GFF1324 at 33.506 kb on + strand, within GFF1324 at 33.511 kb on + strand, within GFF1324 at 33.612 kb on - strand, within GFF1324 at 33.744 kb on - strand at 33.816 kb on + strand at 33.817 kb on - strand at 33.898 kb on + strand, within GFF1325 at 33.899 kb on - strand, within GFF1325 at 33.968 kb on + strand, within GFF1325 at 34.117 kb on - strand, within GFF1325 at 34.286 kb on - strand, within GFF1325 at 34.348 kb on + strand, within GFF1325 at 34.349 kb on - strand, within GFF1325 at 34.369 kb on + strand, within GFF1325 at 34.374 kb on + strand, within GFF1325 at 34.375 kb on - strand, within GFF1325 at 34.422 kb on + strand, within GFF1325 at 34.422 kb on + strand, within GFF1325 at 34.423 kb on - strand, within GFF1325 at 34.442 kb on + strand at 34.442 kb on + strand at 34.542 kb on - strand at 34.611 kb on + strand at 34.751 kb on - strand at 34.838 kb on - strand, within GFF1326 at 34.924 kb on + strand, within GFF1326 at 35.002 kb on - strand, within GFF1326 at 35.108 kb on - strand, within GFF1326 at 35.159 kb on - strand, within GFF1326 at 35.193 kb on + strand, within GFF1326 at 35.193 kb on + strand, within GFF1326 at 35.197 kb on + strand, within GFF1326 at 35.198 kb on - strand, within GFF1326 at 35.242 kb on + strand, within GFF1326 at 35.243 kb on - strand, within GFF1326 at 35.360 kb on - strand, within GFF1326
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.1875 MOI remove 32,883 - -0.6 33,055 + GFF1324 0.18 -0.3 33,094 + GFF1324 0.23 +0.3 33,220 + GFF1324 0.36 -0.7 33,261 - GFF1324 0.40 +0.3 33,370 + GFF1324 0.52 +0.4 33,371 - GFF1324 0.52 +0.2 33,394 + GFF1324 0.55 -0.8 33,506 + GFF1324 0.67 -0.6 33,511 + GFF1324 0.67 +0.3 33,612 - GFF1324 0.78 -0.1 33,744 - -2.3 33,816 + -0.2 33,817 - -0.2 33,898 + GFF1325 0.15 +0.2 33,899 - GFF1325 0.15 -1.4 33,968 + GFF1325 0.25 +0.3 34,117 - GFF1325 0.46 -0.2 34,286 - GFF1325 0.70 +0.1 34,348 + GFF1325 0.79 -2.4 34,349 - GFF1325 0.79 +0.5 34,369 + GFF1325 0.82 -1.0 34,374 + GFF1325 0.82 -0.3 34,375 - GFF1325 0.83 -0.6 34,422 + GFF1325 0.89 -0.2 34,422 + GFF1325 0.89 +0.1 34,423 - GFF1325 0.89 +0.1 34,442 + +0.1 34,442 + -0.4 34,542 - -0.6 34,611 + -0.3 34,751 - -0.2 34,838 - GFF1326 0.16 +0.2 34,924 + GFF1326 0.22 +0.2 35,002 - GFF1326 0.28 -0.0 35,108 - GFF1326 0.35 +0.6 35,159 - GFF1326 0.39 -0.2 35,193 + GFF1326 0.41 -0.4 35,193 + GFF1326 0.41 -1.1 35,197 + GFF1326 0.41 +0.2 35,198 - GFF1326 0.41 +1.0 35,242 + GFF1326 0.45 -0.1 35,243 - GFF1326 0.45 +0.1 35,360 - GFF1326 0.53 -0.3
Or see this region's nucleotide sequence