Experiment: R2A with Oxytetracycline 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0082 and Atu0084 are separated by 265 nucleotides Atu0084 and Atu0085 are separated by 165 nucleotides Atu0085 and Atu0086 are separated by 3 nucleotides Atu0086 and Atu0087 are separated by 75 nucleotides
Atu0082: Atu0082 - polyribonucleotide nucleotidyltransferase, at 84,465 to 86,606
Atu0082
Atu0084: Atu0084 - 30S ribosomal protein S15, at 86,872 to 87,141
Atu0084
Atu0085: Atu0085 - tRNA pseudouridine 55 synthase, at 87,307 to 88,254
Atu0085
Atu0086: Atu0086 - ribosome-binding factor A, at 88,258 to 88,674
Atu0086
Atu0087: Atu0087 - translation initiation factor IF-2, at 88,750 to 91,491
Atu0087
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 87.339 kb on - strand at 87.365 kb on + strand at 87.382 kb on + strand at 87.437 kb on - strand, within Atu0085 at 87.442 kb on - strand, within Atu0085 at 87.452 kb on - strand, within Atu0085 at 87.465 kb on - strand, within Atu0085 at 87.609 kb on - strand, within Atu0085 at 87.751 kb on + strand, within Atu0085 at 87.832 kb on - strand, within Atu0085 at 87.862 kb on - strand, within Atu0085 at 88.213 kb on + strand at 88.221 kb on - strand at 88.221 kb on - strand at 88.221 kb on - strand at 88.221 kb on - strand at 88.456 kb on + strand, within Atu0086 at 88.625 kb on - strand, within Atu0086 at 88.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Oxytetracycline 0.0002 mM remove 87,339 - -0.4 87,365 + +0.5 87,382 + +1.8 87,437 - Atu0085 0.14 +0.8 87,442 - Atu0085 0.14 +0.3 87,452 - Atu0085 0.15 +0.1 87,465 - Atu0085 0.17 +2.0 87,609 - Atu0085 0.32 +0.7 87,751 + Atu0085 0.47 -1.5 87,832 - Atu0085 0.55 -0.1 87,862 - Atu0085 0.59 -0.1 88,213 + -0.1 88,221 - -0.3 88,221 - -1.6 88,221 - -0.5 88,221 - -0.3 88,456 + Atu0086 0.47 -0.8 88,625 - Atu0086 0.88 -2.8 88,768 - +2.3
Or see this region's nucleotide sequence