Experiment: methylene chloride 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recB and ptr overlap by 8 nucleotides ptr and recC are separated by 175 nucleotides
b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025
recB
b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906
ptr
b2822: recC - exonuclease V (RecBCD complex), gamma chain (NCBI), at 2,957,082 to 2,960,450
recC
Position (kb)
2954
2955
2956
2957 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2953.040 kb on - strand, within recB at 2953.978 kb on + strand at 2953.978 kb on + strand at 2954.006 kb on - strand at 2954.072 kb on + strand at 2954.072 kb on + strand at 2954.201 kb on - strand at 2954.359 kb on + strand, within ptr at 2954.508 kb on + strand, within ptr at 2954.516 kb on + strand, within ptr at 2954.587 kb on - strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.713 kb on - strand, within ptr at 2954.724 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.777 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr at 2955.059 kb on + strand, within ptr at 2955.080 kb on - strand, within ptr at 2955.090 kb on + strand, within ptr at 2955.232 kb on - strand, within ptr at 2955.232 kb on - strand, within ptr at 2955.232 kb on - strand, within ptr at 2955.265 kb on - strand, within ptr at 2955.334 kb on + strand, within ptr at 2955.413 kb on - strand, within ptr at 2955.413 kb on - strand, within ptr at 2955.482 kb on - strand, within ptr at 2955.573 kb on + strand, within ptr at 2955.733 kb on + strand, within ptr at 2955.842 kb on - strand, within ptr at 2955.842 kb on - strand, within ptr at 2955.842 kb on - strand, within ptr at 2955.907 kb on + strand, within ptr at 2955.907 kb on + strand, within ptr at 2955.912 kb on + strand, within ptr at 2955.940 kb on + strand, within ptr at 2956.077 kb on - strand, within ptr at 2956.077 kb on - strand, within ptr at 2956.082 kb on - strand, within ptr at 2956.082 kb on - strand, within ptr at 2956.353 kb on - strand, within ptr at 2956.360 kb on + strand, within ptr at 2956.362 kb on + strand, within ptr at 2956.370 kb on - strand, within ptr at 2956.419 kb on - strand, within ptr at 2956.478 kb on - strand, within ptr at 2956.478 kb on - strand, within ptr at 2956.485 kb on + strand, within ptr at 2956.485 kb on + strand, within ptr at 2956.598 kb on - strand, within ptr at 2956.607 kb on - strand, within ptr at 2956.653 kb on + strand at 2956.658 kb on - strand at 2956.658 kb on - strand at 2956.663 kb on + strand at 2956.772 kb on - strand at 2956.772 kb on - strand at 2956.844 kb on + strand at 2956.882 kb on - strand at 2957.218 kb on - strand at 2957.288 kb on + strand at 2957.823 kb on + strand, within recC
Per-strain Table
Position Strand Gene LocusTag Fraction methylene chloride 5 vol% remove 2,953,040 - recB b2820 0.72 -1.7 2,953,978 + -0.5 2,953,978 + -1.2 2,954,006 - -0.5 2,954,072 + +0.1 2,954,072 + +0.3 2,954,201 - +0.5 2,954,359 + ptr b2821 0.12 +0.2 2,954,508 + ptr b2821 0.17 +0.2 2,954,516 + ptr b2821 0.17 -0.1 2,954,587 - ptr b2821 0.20 +0.0 2,954,617 + ptr b2821 0.21 -0.1 2,954,617 + ptr b2821 0.21 +0.7 2,954,626 - ptr b2821 0.21 +0.8 2,954,626 - ptr b2821 0.21 -0.4 2,954,713 - ptr b2821 0.24 +0.4 2,954,724 - ptr b2821 0.24 -0.1 2,954,759 - ptr b2821 0.26 +0.0 2,954,759 - ptr b2821 0.26 -0.0 2,954,771 - ptr b2821 0.26 +0.0 2,954,771 - ptr b2821 0.26 -0.0 2,954,777 - ptr b2821 0.26 +0.2 2,954,782 - ptr b2821 0.26 +0.1 2,954,782 - ptr b2821 0.26 +0.1 2,954,857 + ptr b2821 0.29 -0.0 2,954,857 + ptr b2821 0.29 +0.1 2,954,905 + ptr b2821 0.31 +0.3 2,954,905 + ptr b2821 0.31 +0.9 2,954,985 + ptr b2821 0.33 -0.1 2,954,985 + ptr b2821 0.33 +0.6 2,955,059 + ptr b2821 0.36 -0.2 2,955,080 - ptr b2821 0.37 +0.7 2,955,090 + ptr b2821 0.37 -0.7 2,955,232 - ptr b2821 0.42 +0.7 2,955,232 - ptr b2821 0.42 +0.4 2,955,232 - ptr b2821 0.42 -0.4 2,955,265 - ptr b2821 0.43 -0.4 2,955,334 + ptr b2821 0.46 -0.2 2,955,413 - ptr b2821 0.48 -0.5 2,955,413 - ptr b2821 0.48 +0.3 2,955,482 - ptr b2821 0.51 -0.2 2,955,573 + ptr b2821 0.54 -0.6 2,955,733 + ptr b2821 0.59 -0.1 2,955,842 - ptr b2821 0.63 +0.0 2,955,842 - ptr b2821 0.63 +0.2 2,955,842 - ptr b2821 0.63 -1.9 2,955,907 + ptr b2821 0.65 +0.7 2,955,907 + ptr b2821 0.65 +0.2 2,955,912 + ptr b2821 0.66 -0.1 2,955,940 + ptr b2821 0.67 +0.2 2,956,077 - ptr b2821 0.71 -0.8 2,956,077 - ptr b2821 0.71 -0.1 2,956,082 - ptr b2821 0.71 -1.2 2,956,082 - ptr b2821 0.71 -0.5 2,956,353 - ptr b2821 0.81 +0.3 2,956,360 + ptr b2821 0.81 +0.7 2,956,362 + ptr b2821 0.81 -0.9 2,956,370 - ptr b2821 0.81 +0.4 2,956,419 - ptr b2821 0.83 +0.3 2,956,478 - ptr b2821 0.85 -3.1 2,956,478 - ptr b2821 0.85 +1.0 2,956,485 + ptr b2821 0.85 +0.6 2,956,485 + ptr b2821 0.85 +0.8 2,956,598 - ptr b2821 0.89 +0.9 2,956,607 - ptr b2821 0.90 -0.4 2,956,653 + +0.1 2,956,658 - +1.4 2,956,658 - -1.0 2,956,663 + +0.4 2,956,772 - +0.0 2,956,772 - +0.6 2,956,844 + -0.6 2,956,882 - +0.0 2,957,218 - +0.0 2,957,288 + -0.4 2,957,823 + recC b2822 0.22 -1.7
Or see this region's nucleotide sequence