Experiment: methylene chloride 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insF-5 and gatD are separated by 434 nucleotides gatD and gatC are separated by 47 nucleotides
b2089: insF-5 - IS3 element protein InsF (NCBI), at 2,168,556 to 2,169,422
insF-5
b2091: gatD - galactitol-1-phosphate dehydrogenase, Zn-dependent and NAD(P)-binding (NCBI), at 2,169,857 to 2,170,897
gatD
b2092: gatC - galactitol-specific enzyme IIC component of PTS (NCBI), at 2,170,945 to 2,172,300
gatC
Position (kb)
2169
2170
2171 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2169.429 kb on + strand at 2169.661 kb on - strand at 2169.661 kb on - strand at 2169.691 kb on + strand at 2169.769 kb on - strand at 2169.798 kb on + strand at 2169.836 kb on + strand at 2169.836 kb on + strand at 2169.839 kb on - strand at 2169.839 kb on - strand at 2169.996 kb on + strand, within gatD at 2170.033 kb on - strand, within gatD at 2170.231 kb on - strand, within gatD at 2170.231 kb on - strand, within gatD at 2170.265 kb on + strand, within gatD at 2170.272 kb on - strand, within gatD at 2170.273 kb on - strand, within gatD at 2170.273 kb on - strand, within gatD at 2170.342 kb on - strand, within gatD at 2170.342 kb on - strand, within gatD at 2170.361 kb on + strand, within gatD at 2170.361 kb on + strand, within gatD at 2170.364 kb on - strand, within gatD at 2170.379 kb on + strand, within gatD at 2170.379 kb on + strand, within gatD at 2170.379 kb on - strand, within gatD at 2170.379 kb on - strand, within gatD at 2170.432 kb on - strand, within gatD at 2170.452 kb on - strand, within gatD at 2170.624 kb on - strand, within gatD at 2170.624 kb on - strand, within gatD at 2170.665 kb on - strand, within gatD at 2170.668 kb on + strand, within gatD at 2170.676 kb on - strand, within gatD at 2170.676 kb on - strand at 2170.689 kb on + strand, within gatD at 2170.707 kb on - strand, within gatD at 2170.710 kb on - strand, within gatD at 2170.710 kb on - strand, within gatD at 2170.792 kb on - strand, within gatD at 2170.793 kb on - strand, within gatD at 2170.793 kb on - strand, within gatD at 2170.810 kb on + strand at 2170.810 kb on + strand at 2170.868 kb on - strand at 2170.873 kb on + strand at 2170.873 kb on - strand at 2170.912 kb on + strand at 2170.912 kb on + strand at 2171.087 kb on - strand, within gatC at 2171.226 kb on + strand, within gatC at 2171.404 kb on - strand, within gatC at 2171.562 kb on + strand, within gatC at 2171.566 kb on + strand, within gatC at 2171.583 kb on - strand, within gatC at 2171.732 kb on - strand, within gatC at 2171.762 kb on - strand, within gatC at 2171.762 kb on - strand, within gatC at 2171.771 kb on - strand, within gatC at 2171.771 kb on - strand, within gatC at 2171.871 kb on + strand, within gatC at 2171.871 kb on + strand, within gatC at 2171.879 kb on - strand, within gatC at 2171.879 kb on - strand, within gatC
Per-strain Table
Position Strand Gene LocusTag Fraction methylene chloride 5 vol% remove 2,169,429 + -0.6 2,169,661 - +0.2 2,169,661 - +0.4 2,169,691 + -0.2 2,169,769 - +0.1 2,169,798 + +0.2 2,169,836 + +0.6 2,169,836 + -0.5 2,169,839 - +0.5 2,169,839 - -0.8 2,169,996 + gatD b2091 0.13 +2.9 2,170,033 - gatD b2091 0.17 +0.3 2,170,231 - gatD b2091 0.36 +1.4 2,170,231 - gatD b2091 0.36 +0.4 2,170,265 + gatD b2091 0.39 -0.1 2,170,272 - gatD b2091 0.40 -0.4 2,170,273 - gatD b2091 0.40 +0.5 2,170,273 - gatD b2091 0.40 +0.0 2,170,342 - gatD b2091 0.47 +0.1 2,170,342 - gatD b2091 0.47 -0.1 2,170,361 + gatD b2091 0.48 +0.5 2,170,361 + gatD b2091 0.48 -0.7 2,170,364 - gatD b2091 0.49 +0.3 2,170,379 + gatD b2091 0.50 +0.4 2,170,379 + gatD b2091 0.50 -1.3 2,170,379 - gatD b2091 0.50 +0.8 2,170,379 - gatD b2091 0.50 -0.0 2,170,432 - gatD b2091 0.55 +0.5 2,170,452 - gatD b2091 0.57 +0.4 2,170,624 - gatD b2091 0.74 +0.6 2,170,624 - gatD b2091 0.74 -0.1 2,170,665 - gatD b2091 0.78 +0.7 2,170,668 + gatD b2091 0.78 -0.5 2,170,676 - gatD b2091 0.79 -0.0 2,170,676 - +0.8 2,170,689 + gatD b2091 0.80 +0.8 2,170,707 - gatD b2091 0.82 -0.6 2,170,710 - gatD b2091 0.82 +0.4 2,170,710 - gatD b2091 0.82 -0.2 2,170,792 - gatD b2091 0.90 -0.6 2,170,793 - gatD b2091 0.90 -0.1 2,170,793 - gatD b2091 0.90 +0.6 2,170,810 + +0.4 2,170,810 + +0.6 2,170,868 - -0.1 2,170,873 + +0.2 2,170,873 - +0.1 2,170,912 + +0.1 2,170,912 + +0.4 2,171,087 - gatC b2092 0.10 -0.3 2,171,226 + gatC b2092 0.21 +0.2 2,171,404 - gatC b2092 0.34 -0.7 2,171,562 + gatC b2092 0.46 +0.9 2,171,566 + gatC b2092 0.46 +0.4 2,171,583 - gatC b2092 0.47 -0.6 2,171,732 - gatC b2092 0.58 -0.7 2,171,762 - gatC b2092 0.60 +1.1 2,171,762 - gatC b2092 0.60 +1.0 2,171,771 - gatC b2092 0.61 +0.7 2,171,771 - gatC b2092 0.61 +0.4 2,171,871 + gatC b2092 0.68 +0.6 2,171,871 + gatC b2092 0.68 +0.0 2,171,879 - gatC b2092 0.69 +0.2 2,171,879 - gatC b2092 0.69 -0.1
Or see this region's nucleotide sequence