Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF92 and GFF93 are separated by 94 nucleotides GFF93 and GFF94 are separated by 6 nucleotides GFF94 and GFF95 are separated by 61 nucleotides GFF95 and GFF96 are separated by 370 nucleotides
GFF92 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 76,730 to 77,584
GFF92
GFF93 - Aspartate 1-decarboxylase (EC 4.1.1.11), at 77,679 to 78,059
GFF93
GFF94 - Polysaccharide deacetylase, at 78,066 to 79,295
GFF94
GFF95 - Putative PTS system IIA component yadI (EC 2.7.1.69), at 79,357 to 79,797
GFF95
GFF96 - Cell wall surface anchor family protein, at 80,168 to 80,707
GFF96
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 77.297 kb on - strand, within GFF92 at 77.304 kb on - strand, within GFF92 at 77.342 kb on + strand, within GFF92 at 77.356 kb on + strand, within GFF92 at 77.357 kb on - strand, within GFF92 at 77.405 kb on - strand, within GFF92 at 77.649 kb on - strand at 77.789 kb on + strand, within GFF93 at 77.813 kb on + strand, within GFF93 at 77.851 kb on - strand, within GFF93 at 78.060 kb on + strand at 78.131 kb on - strand at 78.270 kb on + strand, within GFF94 at 78.335 kb on - strand, within GFF94 at 78.348 kb on + strand, within GFF94 at 78.625 kb on + strand, within GFF94 at 78.625 kb on + strand, within GFF94 at 78.625 kb on + strand, within GFF94 at 78.625 kb on + strand, within GFF94 at 78.632 kb on - strand, within GFF94 at 78.632 kb on - strand, within GFF94 at 78.632 kb on - strand, within GFF94 at 78.646 kb on + strand, within GFF94 at 78.759 kb on + strand, within GFF94 at 78.773 kb on - strand, within GFF94 at 78.823 kb on + strand, within GFF94 at 78.823 kb on + strand, within GFF94 at 78.867 kb on + strand, within GFF94 at 79.106 kb on - strand, within GFF94 at 79.108 kb on + strand, within GFF94 at 79.222 kb on + strand at 79.275 kb on - strand at 79.295 kb on + strand at 79.296 kb on - strand at 79.296 kb on - strand at 79.296 kb on - strand at 79.296 kb on - strand at 79.297 kb on + strand at 79.385 kb on - strand at 79.672 kb on - strand, within GFF95 at 79.676 kb on - strand, within GFF95 at 79.676 kb on - strand, within GFF95 at 80.259 kb on + strand, within GFF96 at 80.260 kb on - strand, within GFF96 at 80.263 kb on + strand, within GFF96
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 77,297 - GFF92 0.66 -0.7 77,304 - GFF92 0.67 -0.2 77,342 + GFF92 0.72 -0.3 77,356 + GFF92 0.73 -0.4 77,357 - GFF92 0.73 -1.1 77,405 - GFF92 0.79 -1.0 77,649 - +0.6 77,789 + GFF93 0.29 +0.1 77,813 + GFF93 0.35 -0.1 77,851 - GFF93 0.45 -1.1 78,060 + -3.8 78,131 - -1.1 78,270 + GFF94 0.17 +0.5 78,335 - GFF94 0.22 +1.8 78,348 + GFF94 0.23 +0.8 78,625 + GFF94 0.45 -0.3 78,625 + GFF94 0.45 -0.7 78,625 + GFF94 0.45 +0.7 78,625 + GFF94 0.45 -0.3 78,632 - GFF94 0.46 +0.7 78,632 - GFF94 0.46 +0.1 78,632 - GFF94 0.46 -0.4 78,646 + GFF94 0.47 +0.1 78,759 + GFF94 0.56 +0.3 78,773 - GFF94 0.57 +0.4 78,823 + GFF94 0.62 -0.6 78,823 + GFF94 0.62 -0.5 78,867 + GFF94 0.65 +0.3 79,106 - GFF94 0.85 +0.5 79,108 + GFF94 0.85 -0.1 79,222 + +0.2 79,275 - -0.3 79,295 + +0.6 79,296 - -0.6 79,296 - +0.0 79,296 - +1.3 79,296 - +1.9 79,297 + +0.1 79,385 - +0.1 79,672 - GFF95 0.71 -0.5 79,676 - GFF95 0.72 -0.0 79,676 - GFF95 0.72 -0.7 80,259 + GFF96 0.17 -2.6 80,260 - GFF96 0.17 +0.4 80,263 + GFF96 0.18 +0.6
Or see this region's nucleotide sequence