Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF832 and GFF833 are separated by 90 nucleotides GFF833 and GFF834 are separated by 131 nucleotides GFF834 and GFF835 are separated by 14 nucleotides GFF835 and GFF836 overlap by 1 nucleotides
GFF832 - '3'-to-5' exoribonuclease RNase R' transl_table=11, at 14,413 to 16,851
GFF832
GFF833 - '23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-)' transl_table=11, at 16,942 to 17,673
GFF833
GFF834 - FIG006163: hypothetical protein, at 17,805 to 18,209
GFF834
GFF835 - PspA/IM30 family protein, at 18,224 to 18,922
GFF835
GFF836 - Putative potassium channel protein, at 18,922 to 19,992
GFF836
Position (kb) 17
18
19 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 17.303 kb on + strand, within GFF833 at 17.303 kb on + strand, within GFF833 at 17.304 kb on - strand, within GFF833 at 17.304 kb on - strand, within GFF833 at 17.481 kb on + strand, within GFF833 at 17.545 kb on - strand, within GFF833 at 17.968 kb on - strand, within GFF834 at 18.084 kb on + strand, within GFF834 at 18.196 kb on - strand at 18.256 kb on + strand at 18.306 kb on + strand, within GFF835 at 18.306 kb on + strand, within GFF835 at 18.307 kb on - strand, within GFF835 at 18.311 kb on - strand, within GFF835 at 18.326 kb on - strand, within GFF835 at 18.469 kb on + strand, within GFF835 at 18.725 kb on - strand, within GFF835 at 18.920 kb on + strand at 18.921 kb on - strand at 19.021 kb on + strand at 19.068 kb on - strand, within GFF836 at 19.082 kb on - strand, within GFF836 at 19.084 kb on + strand, within GFF836
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 17,303 + GFF833 0.49 -0.2 17,303 + GFF833 0.49 -0.7 17,304 - GFF833 0.49 -1.2 17,304 - GFF833 0.49 +0.7 17,481 + GFF833 0.74 -0.1 17,545 - GFF833 0.82 -3.8 17,968 - GFF834 0.40 -1.3 18,084 + GFF834 0.69 -0.5 18,196 - -0.4 18,256 + -0.4 18,306 + GFF835 0.12 +0.1 18,306 + GFF835 0.12 -2.4 18,307 - GFF835 0.12 +0.5 18,311 - GFF835 0.12 -0.7 18,326 - GFF835 0.15 +0.5 18,469 + GFF835 0.35 -0.8 18,725 - GFF835 0.72 -2.3 18,920 + +1.7 18,921 - -1.1 19,021 + -0.6 19,068 - GFF836 0.14 -1.2 19,082 - GFF836 0.15 +0.4 19,084 + GFF836 0.15 -0.1
Or see this region's nucleotide sequence