Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF822 and GFF823 are separated by 9 nucleotides GFF823 and GFF824 are separated by 37 nucleotides
GFF822 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 3,714 to 5,033
GFF822
GFF823 - DNA mismatch repair protein MutL, at 5,043 to 6,899
GFF823
GFF824 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 6,937 to 7,842
GFF824
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4.068 kb on + strand, within GFF822 at 4.069 kb on - strand, within GFF822 at 4.069 kb on - strand, within GFF822 at 4.372 kb on + strand, within GFF822 at 4.382 kb on - strand, within GFF822 at 4.382 kb on - strand, within GFF822 at 4.389 kb on - strand, within GFF822 at 4.403 kb on + strand, within GFF822 at 4.621 kb on + strand, within GFF822 at 4.666 kb on - strand, within GFF822 at 4.719 kb on + strand, within GFF822 at 4.720 kb on - strand, within GFF822 at 4.720 kb on - strand, within GFF822 at 4.739 kb on - strand, within GFF822 at 4.739 kb on - strand, within GFF822 at 4.794 kb on - strand, within GFF822 at 4.916 kb on + strand at 4.917 kb on - strand at 4.917 kb on - strand at 4.918 kb on + strand at 4.919 kb on - strand at 4.921 kb on - strand at 5.202 kb on - strand at 5.309 kb on + strand, within GFF823 at 5.372 kb on - strand, within GFF823 at 5.497 kb on - strand, within GFF823 at 5.502 kb on - strand, within GFF823 at 5.546 kb on + strand, within GFF823 at 5.812 kb on - strand, within GFF823 at 6.009 kb on + strand, within GFF823 at 6.009 kb on + strand, within GFF823 at 6.010 kb on - strand, within GFF823 at 6.010 kb on - strand, within GFF823 at 6.286 kb on + strand, within GFF823 at 6.344 kb on + strand, within GFF823 at 6.345 kb on - strand, within GFF823 at 6.468 kb on - strand, within GFF823 at 6.468 kb on - strand, within GFF823 at 6.634 kb on + strand, within GFF823 at 6.679 kb on + strand, within GFF823 at 6.684 kb on + strand, within GFF823 at 6.827 kb on - strand at 6.851 kb on - strand at 6.902 kb on + strand at 6.903 kb on - strand at 6.921 kb on + strand at 6.975 kb on - strand at 6.998 kb on + strand at 7.007 kb on + strand at 7.107 kb on - strand, within GFF824 at 7.212 kb on + strand, within GFF824 at 7.347 kb on - strand, within GFF824 at 7.660 kb on + strand, within GFF824 at 7.660 kb on + strand, within GFF824 at 7.681 kb on + strand, within GFF824 at 7.682 kb on - strand, within GFF824 at 7.686 kb on + strand, within GFF824 at 7.811 kb on + strand at 7.814 kb on + strand at 7.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 4,068 + GFF822 0.27 -0.3 4,069 - GFF822 0.27 +0.4 4,069 - GFF822 0.27 +0.6 4,372 + GFF822 0.50 -0.6 4,382 - GFF822 0.51 -0.6 4,382 - GFF822 0.51 -1.5 4,389 - GFF822 0.51 -2.3 4,403 + GFF822 0.52 -0.5 4,621 + GFF822 0.69 -1.3 4,666 - GFF822 0.72 +0.8 4,719 + GFF822 0.76 +0.5 4,720 - GFF822 0.76 -0.9 4,720 - GFF822 0.76 -1.4 4,739 - GFF822 0.78 +0.1 4,739 - GFF822 0.78 +1.2 4,794 - GFF822 0.82 -0.8 4,916 + -1.5 4,917 - +0.8 4,917 - -1.0 4,918 + +0.5 4,919 - -0.0 4,921 - -0.1 5,202 - -0.2 5,309 + GFF823 0.14 -1.7 5,372 - GFF823 0.18 -0.2 5,497 - GFF823 0.24 +0.3 5,502 - GFF823 0.25 -0.3 5,546 + GFF823 0.27 -0.6 5,812 - GFF823 0.41 -0.6 6,009 + GFF823 0.52 -0.9 6,009 + GFF823 0.52 +0.4 6,010 - GFF823 0.52 -0.8 6,010 - GFF823 0.52 +0.3 6,286 + GFF823 0.67 -2.1 6,344 + GFF823 0.70 +1.4 6,345 - GFF823 0.70 +0.6 6,468 - GFF823 0.77 -2.1 6,468 - GFF823 0.77 -1.3 6,634 + GFF823 0.86 -0.1 6,679 + GFF823 0.88 -1.9 6,684 + GFF823 0.88 -1.9 6,827 - +0.1 6,851 - +0.4 6,902 + -1.3 6,903 - -1.3 6,921 + -2.0 6,975 - -1.2 6,998 + +0.4 7,007 + -0.6 7,107 - GFF824 0.19 -2.6 7,212 + GFF824 0.30 +0.4 7,347 - GFF824 0.45 -1.0 7,660 + GFF824 0.80 -3.5 7,660 + GFF824 0.80 -1.2 7,681 + GFF824 0.82 -1.9 7,682 - GFF824 0.82 -3.6 7,686 + GFF824 0.83 -1.9 7,811 + +0.7 7,814 + -3.1 7,849 - +4.4
Or see this region's nucleotide sequence