Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF788 and GFF789 are separated by 182 nucleotides GFF789 and GFF790 overlap by 1 nucleotides GFF790 and GFF791 overlap by 4 nucleotides
GFF788 - ATP-dependent RNA helicase YejH, at 137,018 to 138,646
GFF788
GFF789 - DNA primase (EC 2.7.7.-), phage-associated, at 138,829 to 139,611
GFF789
GFF790 - FIG01220455: hypothetical protein, at 139,611 to 140,471
GFF790
GFF791 - Phage antitermination protein Q, at 140,468 to 141,283
GFF791
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 138.622 kb on + strand at 138.809 kb on + strand at 138.885 kb on - strand at 138.950 kb on + strand, within GFF789 at 139.039 kb on - strand, within GFF789 at 139.095 kb on - strand, within GFF789 at 139.108 kb on + strand, within GFF789 at 139.119 kb on + strand, within GFF789 at 139.145 kb on - strand, within GFF789 at 139.228 kb on - strand, within GFF789 at 139.232 kb on - strand, within GFF789 at 139.232 kb on - strand, within GFF789 at 139.232 kb on - strand, within GFF789 at 139.232 kb on - strand, within GFF789 at 139.333 kb on + strand, within GFF789 at 139.463 kb on - strand, within GFF789 at 139.548 kb on + strand at 139.549 kb on - strand at 139.616 kb on + strand at 139.616 kb on + strand at 139.617 kb on - strand at 139.634 kb on + strand at 139.682 kb on - strand at 139.710 kb on - strand, within GFF790 at 139.710 kb on - strand, within GFF790 at 139.849 kb on - strand, within GFF790 at 139.916 kb on + strand, within GFF790 at 139.980 kb on - strand, within GFF790 at 140.030 kb on + strand, within GFF790 at 140.079 kb on - strand, within GFF790 at 140.152 kb on + strand, within GFF790 at 140.161 kb on - strand, within GFF790 at 140.170 kb on + strand, within GFF790 at 140.170 kb on + strand, within GFF790 at 140.171 kb on - strand, within GFF790 at 140.187 kb on - strand, within GFF790 at 140.412 kb on + strand at 140.414 kb on + strand at 140.566 kb on + strand, within GFF791 at 140.568 kb on + strand, within GFF791 at 140.746 kb on + strand, within GFF791 at 140.746 kb on + strand, within GFF791 at 140.747 kb on - strand, within GFF791 at 140.747 kb on - strand, within GFF791 at 140.747 kb on - strand, within GFF791 at 140.892 kb on - strand, within GFF791 at 141.226 kb on - strand at 141.230 kb on + strand at 141.231 kb on - strand at 141.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 138,622 + -0.0 138,809 + +1.0 138,885 - -0.3 138,950 + GFF789 0.15 +0.2 139,039 - GFF789 0.27 +1.6 139,095 - GFF789 0.34 -0.9 139,108 + GFF789 0.36 +0.2 139,119 + GFF789 0.37 +0.0 139,145 - GFF789 0.40 +0.8 139,228 - GFF789 0.51 +0.9 139,232 - GFF789 0.51 +1.7 139,232 - GFF789 0.51 -0.5 139,232 - GFF789 0.51 -2.0 139,232 - GFF789 0.51 +0.4 139,333 + GFF789 0.64 +0.6 139,463 - GFF789 0.81 +0.6 139,548 + -0.1 139,549 - -0.3 139,616 + +0.6 139,616 + +0.5 139,617 - +0.7 139,634 + +1.4 139,682 - -0.3 139,710 - GFF790 0.11 -1.9 139,710 - GFF790 0.11 +1.1 139,849 - GFF790 0.28 +1.1 139,916 + GFF790 0.35 +2.0 139,980 - GFF790 0.43 +0.9 140,030 + GFF790 0.49 +1.0 140,079 - GFF790 0.54 +0.0 140,152 + GFF790 0.63 +0.1 140,161 - GFF790 0.64 +1.0 140,170 + GFF790 0.65 +1.5 140,170 + GFF790 0.65 -1.1 140,171 - GFF790 0.65 +2.6 140,187 - GFF790 0.67 +2.6 140,412 + -3.0 140,414 + +0.6 140,566 + GFF791 0.12 +1.4 140,568 + GFF791 0.12 +0.5 140,746 + GFF791 0.34 +2.2 140,746 + GFF791 0.34 -0.4 140,747 - GFF791 0.34 +0.6 140,747 - GFF791 0.34 +1.6 140,747 - GFF791 0.34 -2.2 140,892 - GFF791 0.52 +1.0 141,226 - +1.2 141,230 + +0.6 141,231 - +2.0 141,358 - +1.5
Or see this region's nucleotide sequence