Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF75 and GFF76 are separated by 410 nucleotides GFF76 and GFF77 are separated by 160 nucleotides GFF77 and GFF78 are separated by 36 nucleotides GFF78 and GFF79 overlap by 4 nucleotides GFF79 and GFF80 overlap by 1 nucleotides
GFF75 - Major fimbrial subunit StfA, at 57,872 to 58,432
GFF75
GFF76 - hypothetical protein, at 58,843 to 58,968
GFF76
GFF77 - FIG01046082: hypothetical protein, at 59,129 to 59,335
GFF77
GFF78 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), permease component FhuB, at 59,372 to 61,429
GFF78
GFF79 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), periplasmic substrate binding protein FhuD, at 61,426 to 62,316
GFF79
GFF80 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), ATP-binding protein FhuC, at 62,316 to 63,113
GFF80
Position (kb) 59
60
61
62 Strain fitness (log2 ratio) -1
0
1
2 at 59.387 kb on - strand at 59.406 kb on + strand at 59.805 kb on + strand, within GFF78 at 59.810 kb on - strand, within GFF78 at 59.832 kb on - strand, within GFF78 at 59.959 kb on + strand, within GFF78 at 59.959 kb on + strand, within GFF78 at 59.962 kb on + strand, within GFF78 at 60.125 kb on - strand, within GFF78 at 60.473 kb on - strand, within GFF78 at 60.866 kb on + strand, within GFF78 at 61.110 kb on - strand, within GFF78 at 61.235 kb on - strand at 61.374 kb on - strand at 61.374 kb on - strand at 61.449 kb on + strand at 61.493 kb on + strand at 61.493 kb on + strand at 61.494 kb on - strand at 61.626 kb on + strand, within GFF79 at 61.627 kb on - strand, within GFF79 at 61.628 kb on + strand, within GFF79 at 61.629 kb on - strand, within GFF79 at 61.755 kb on + strand, within GFF79 at 61.756 kb on - strand, within GFF79 at 61.845 kb on - strand, within GFF79 at 61.845 kb on - strand, within GFF79 at 61.945 kb on - strand, within GFF79 at 62.123 kb on + strand, within GFF79 at 62.124 kb on - strand, within GFF79 at 62.281 kb on + strand at 62.295 kb on + strand at 62.304 kb on - strand at 62.317 kb on + strand at 62.318 kb on - strand at 62.318 kb on - strand at 62.320 kb on + strand at 62.320 kb on + strand at 62.381 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 59,387 - +0.1 59,406 + -0.2 59,805 + GFF78 0.21 -0.6 59,810 - GFF78 0.21 -1.2 59,832 - GFF78 0.22 -0.7 59,959 + GFF78 0.29 -0.1 59,959 + GFF78 0.29 +1.4 59,962 + GFF78 0.29 -0.0 60,125 - GFF78 0.37 +1.1 60,473 - GFF78 0.53 +0.2 60,866 + GFF78 0.73 -0.7 61,110 - GFF78 0.84 +1.1 61,235 - +0.8 61,374 - +0.8 61,374 - -0.7 61,449 + -1.7 61,493 + -0.7 61,493 + +0.2 61,494 - -0.2 61,626 + GFF79 0.22 +0.1 61,627 - GFF79 0.23 +0.4 61,628 + GFF79 0.23 +1.8 61,629 - GFF79 0.23 +0.7 61,755 + GFF79 0.37 +0.1 61,756 - GFF79 0.37 -0.7 61,845 - GFF79 0.47 +0.4 61,845 - GFF79 0.47 +0.6 61,945 - GFF79 0.58 +0.7 62,123 + GFF79 0.78 +0.8 62,124 - GFF79 0.78 +0.7 62,281 + -0.3 62,295 + -0.0 62,304 - +0.1 62,317 + +1.0 62,318 - +1.4 62,318 - +1.3 62,320 + +0.9 62,320 + -0.2 62,381 - -0.1
Or see this region's nucleotide sequence