Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF702 and GFF703 overlap by 4 nucleotides GFF703 and GFF704 are separated by 118 nucleotides GFF704 and GFF705 overlap by 4 nucleotides
GFF702 - Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51), at 58,542 to 59,468
GFF702
GFF703 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 59,465 to 61,687
GFF703
GFF704 - Glutamate transport ATP-binding protein, at 61,806 to 62,528
GFF704
GFF705 - Glutamate transport membrane-spanning protein, at 62,525 to 63,184
GFF705
Position (kb) 59
60
61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 58.485 kb on - strand at 58.485 kb on - strand at 58.487 kb on - strand at 58.491 kb on - strand at 58.710 kb on - strand, within GFF702 at 58.797 kb on + strand, within GFF702 at 58.797 kb on + strand, within GFF702 at 58.799 kb on + strand, within GFF702 at 58.800 kb on - strand, within GFF702 at 58.893 kb on + strand, within GFF702 at 58.917 kb on + strand, within GFF702 at 59.064 kb on - strand, within GFF702 at 59.210 kb on - strand, within GFF702 at 59.423 kb on + strand at 59.451 kb on - strand at 59.535 kb on + strand at 59.561 kb on - strand at 59.591 kb on - strand at 59.669 kb on + strand at 59.802 kb on + strand, within GFF703 at 59.872 kb on + strand, within GFF703 at 59.872 kb on + strand, within GFF703 at 59.948 kb on + strand, within GFF703 at 60.044 kb on - strand, within GFF703 at 60.330 kb on - strand, within GFF703 at 60.544 kb on + strand, within GFF703 at 60.717 kb on - strand, within GFF703 at 60.881 kb on - strand, within GFF703 at 61.064 kb on - strand, within GFF703 at 61.088 kb on + strand, within GFF703 at 61.209 kb on - strand, within GFF703 at 61.726 kb on - strand at 61.727 kb on + strand at 61.727 kb on + strand at 61.900 kb on + strand, within GFF704 at 62.384 kb on + strand, within GFF704 at 62.391 kb on - strand, within GFF704 at 62.391 kb on - strand, within GFF704 at 62.397 kb on + strand, within GFF704 at 62.401 kb on - strand, within GFF704 at 62.474 kb on + strand at 62.475 kb on - strand at 62.475 kb on - strand at 62.478 kb on + strand at 62.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 58,485 - +2.2 58,485 - -2.0 58,487 - -0.3 58,491 - +1.7 58,710 - GFF702 0.18 +0.1 58,797 + GFF702 0.28 -2.0 58,797 + GFF702 0.28 -0.7 58,799 + GFF702 0.28 +1.2 58,800 - GFF702 0.28 +0.3 58,893 + GFF702 0.38 +0.2 58,917 + GFF702 0.40 +0.5 59,064 - GFF702 0.56 -1.0 59,210 - GFF702 0.72 +0.7 59,423 + +0.5 59,451 - +0.6 59,535 + +0.2 59,561 - -0.4 59,591 - +0.9 59,669 + -0.6 59,802 + GFF703 0.15 +1.5 59,872 + GFF703 0.18 +0.2 59,872 + GFF703 0.18 -0.2 59,948 + GFF703 0.22 +1.1 60,044 - GFF703 0.26 +0.1 60,330 - GFF703 0.39 -0.7 60,544 + GFF703 0.49 +0.1 60,717 - GFF703 0.56 -0.1 60,881 - GFF703 0.64 +0.4 61,064 - GFF703 0.72 +0.2 61,088 + GFF703 0.73 -0.4 61,209 - GFF703 0.78 +1.0 61,726 - +0.6 61,727 + -1.0 61,727 + +0.4 61,900 + GFF704 0.13 -1.2 62,384 + GFF704 0.80 -0.1 62,391 - GFF704 0.81 -0.8 62,391 - GFF704 0.81 -0.6 62,397 + GFF704 0.82 -0.0 62,401 - GFF704 0.82 +0.7 62,474 + -0.1 62,475 - -0.2 62,475 - +0.8 62,478 + +0.1 62,479 - +0.3
Or see this region's nucleotide sequence