Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF647

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF646 and GFF647 overlap by 1 nucleotidesGFF647 and GFF648 are separated by 90 nucleotides GFF646 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 1,577 to 2,560 GFF646 GFF647 - Ferrichrome transport ATP-binding protein FhuC (TC 3.A.1.14.3), at 2,560 to 3,336 GFF647 GFF648 - Phosphoglycerate mutase (EC 5.4.2.1), at 3,427 to 4,179 GFF648 Position (kb) 2 3 4Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1.581 kb on + strandat 1.701 kb on + strand, within GFF646at 1.888 kb on + strand, within GFF646at 1.969 kb on + strand, within GFF646at 1.969 kb on + strand, within GFF646at 2.057 kb on - strand, within GFF646at 2.164 kb on + strand, within GFF646at 2.288 kb on - strand, within GFF646at 2.338 kb on + strand, within GFF646at 2.339 kb on - strand, within GFF646at 2.370 kb on + strand, within GFF646at 2.464 kb on + strandat 2.535 kb on + strandat 2.535 kb on + strandat 2.536 kb on - strandat 2.686 kb on - strand, within GFF647at 2.696 kb on + strand, within GFF647at 2.829 kb on - strand, within GFF647at 2.840 kb on + strand, within GFF647at 2.861 kb on + strand, within GFF647at 2.909 kb on + strand, within GFF647at 2.936 kb on + strand, within GFF647at 2.957 kb on - strand, within GFF647at 2.991 kb on - strand, within GFF647at 3.083 kb on + strand, within GFF647at 3.083 kb on + strand, within GFF647at 3.120 kb on + strand, within GFF647at 3.150 kb on + strand, within GFF647at 3.154 kb on - strand, within GFF647at 3.189 kb on - strand, within GFF647at 3.342 kb on - strandat 3.342 kb on - strandat 3.404 kb on - strandat 3.404 kb on - strandat 3.404 kb on - strandat 3.497 kb on + strandat 3.498 kb on - strandat 3.498 kb on - strandat 3.533 kb on + strand, within GFF648at 3.533 kb on + strand, within GFF648at 3.592 kb on - strand, within GFF648at 3.970 kb on + strand, within GFF648at 3.971 kb on - strand, within GFF648at 4.103 kb on + strand, within GFF648at 4.172 kb on - strandat 4.192 kb on - strandat 4.194 kb on - strandat 4.194 kb on - strandat 4.194 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
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1,581 + +0.4
1,701 + GFF646 0.13 +0.4
1,888 + GFF646 0.32 -1.3
1,969 + GFF646 0.40 -0.2
1,969 + GFF646 0.40 +0.2
2,057 - GFF646 0.49 -0.1
2,164 + GFF646 0.60 -0.2
2,288 - GFF646 0.72 +0.8
2,338 + GFF646 0.77 +2.3
2,339 - GFF646 0.77 +0.9
2,370 + GFF646 0.81 -2.8
2,464 + -0.7
2,535 + +1.6
2,535 + +1.0
2,536 - +1.6
2,686 - GFF647 0.16 +0.2
2,696 + GFF647 0.18 -0.3
2,829 - GFF647 0.35 +0.3
2,840 + GFF647 0.36 -0.1
2,861 + GFF647 0.39 +1.6
2,909 + GFF647 0.45 +0.4
2,936 + GFF647 0.48 -0.3
2,957 - GFF647 0.51 +1.1
2,991 - GFF647 0.55 +0.8
3,083 + GFF647 0.67 +0.7
3,083 + GFF647 0.67 -0.2
3,120 + GFF647 0.72 -0.8
3,150 + GFF647 0.76 +0.6
3,154 - GFF647 0.76 -1.6
3,189 - GFF647 0.81 +1.4
3,342 - +3.2
3,342 - +0.2
3,404 - +1.0
3,404 - -0.6
3,404 - -0.7
3,497 + -3.0
3,498 - +1.2
3,498 - -1.1
3,533 + GFF648 0.14 +0.3
3,533 + GFF648 0.14 -2.1
3,592 - GFF648 0.22 -0.3
3,970 + GFF648 0.72 -0.4
3,971 - GFF648 0.72 +1.7
4,103 + GFF648 0.90 -0.3
4,172 - -0.8
4,192 - -0.2
4,194 - -1.2
4,194 - +0.0
4,194 - -0.5

Or see this region's nucleotide sequence