Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF60 and GFF61 are separated by 129 nucleotides GFF61 and GFF62 are separated by 84 nucleotides GFF62 and GFF63 are separated by 4 nucleotides
GFF60 - HtrA protease/chaperone protein, at 43,170 to 44,597
GFF60
GFF61 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), subgroup 1, at 44,727 to 46,244
GFF61
GFF62 - '5'-methylthioadenosine nucleosidase (EC 3.2.2.16) / S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)' transl_table=11, at 46,329 to 47,027
GFF62
GFF63 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 47,032 to 47,820
GFF63
Position (kb) 44
45
46
47 Strain fitness (log2 ratio) -2
-1
0
1
2 at 43.785 kb on - strand, within GFF60 at 43.942 kb on - strand, within GFF60 at 44.027 kb on + strand, within GFF60 at 44.028 kb on - strand, within GFF60 at 44.028 kb on - strand, within GFF60 at 44.029 kb on + strand, within GFF60 at 44.029 kb on + strand, within GFF60 at 44.030 kb on - strand, within GFF60 at 44.172 kb on + strand, within GFF60 at 44.402 kb on + strand, within GFF60 at 44.403 kb on - strand, within GFF60 at 44.418 kb on - strand, within GFF60 at 44.418 kb on - strand, within GFF60 at 44.430 kb on - strand, within GFF60 at 44.581 kb on - strand at 44.640 kb on - strand at 44.992 kb on - strand, within GFF61 at 45.005 kb on - strand, within GFF61 at 45.012 kb on + strand, within GFF61 at 45.012 kb on + strand, within GFF61 at 45.188 kb on + strand, within GFF61 at 45.227 kb on + strand, within GFF61 at 45.279 kb on - strand, within GFF61 at 45.504 kb on - strand, within GFF61 at 45.504 kb on - strand, within GFF61 at 45.519 kb on - strand, within GFF61 at 45.538 kb on + strand, within GFF61 at 45.609 kb on - strand, within GFF61 at 45.641 kb on + strand, within GFF61 at 45.641 kb on + strand, within GFF61 at 45.641 kb on + strand, within GFF61 at 45.642 kb on - strand, within GFF61 at 45.662 kb on + strand, within GFF61 at 45.666 kb on + strand, within GFF61 at 45.666 kb on + strand, within GFF61 at 45.708 kb on - strand, within GFF61 at 45.732 kb on - strand, within GFF61 at 46.053 kb on - strand, within GFF61 at 46.053 kb on - strand, within GFF61 at 46.111 kb on - strand at 46.113 kb on - strand at 46.127 kb on + strand at 46.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 43,785 - GFF60 0.43 +0.4 43,942 - GFF60 0.54 -0.5 44,027 + GFF60 0.60 +1.4 44,028 - GFF60 0.60 -0.5 44,028 - GFF60 0.60 -1.2 44,029 + GFF60 0.60 +1.9 44,029 + GFF60 0.60 -0.5 44,030 - GFF60 0.60 +0.0 44,172 + GFF60 0.70 -1.7 44,402 + GFF60 0.86 +0.0 44,403 - GFF60 0.86 -1.1 44,418 - GFF60 0.87 +1.8 44,418 - GFF60 0.87 +2.4 44,430 - GFF60 0.88 -2.2 44,581 - +1.0 44,640 - -0.1 44,992 - GFF61 0.17 +0.9 45,005 - GFF61 0.18 +0.5 45,012 + GFF61 0.19 +1.3 45,012 + GFF61 0.19 +0.9 45,188 + GFF61 0.30 -0.0 45,227 + GFF61 0.33 +1.4 45,279 - GFF61 0.36 +1.0 45,504 - GFF61 0.51 +1.6 45,504 - GFF61 0.51 -0.1 45,519 - GFF61 0.52 +0.9 45,538 + GFF61 0.53 -0.2 45,609 - GFF61 0.58 -1.1 45,641 + GFF61 0.60 +1.5 45,641 + GFF61 0.60 +1.8 45,641 + GFF61 0.60 +1.4 45,642 - GFF61 0.60 +0.7 45,662 + GFF61 0.62 -0.7 45,666 + GFF61 0.62 -2.0 45,666 + GFF61 0.62 -0.5 45,708 - GFF61 0.65 +2.4 45,732 - GFF61 0.66 +0.6 46,053 - GFF61 0.87 +2.6 46,053 - GFF61 0.87 +1.4 46,111 - +0.6 46,113 - +0.6 46,127 + +0.6 46,294 + +1.6
Or see this region's nucleotide sequence