Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF577 and GFF578 overlap by 1 nucleotides GFF578 and GFF579 are separated by 19 nucleotides
GFF577 - Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27), at 308,971 to 310,266
GFF577
GFF578 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 310,266 to 311,699
GFF578
GFF579 - Glycogen phosphorylase (EC 2.4.1.1), at 311,719 to 314,166
GFF579
Position (kb)
310
311
312 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 309.291 kb on + strand, within GFF577 at 309.351 kb on + strand, within GFF577 at 309.351 kb on + strand, within GFF577 at 309.352 kb on - strand, within GFF577 at 309.374 kb on - strand, within GFF577 at 309.400 kb on - strand, within GFF577 at 309.400 kb on - strand, within GFF577 at 309.403 kb on + strand, within GFF577 at 309.404 kb on - strand, within GFF577 at 309.424 kb on - strand, within GFF577 at 309.424 kb on - strand, within GFF577 at 309.472 kb on - strand, within GFF577 at 309.640 kb on + strand, within GFF577 at 309.641 kb on - strand, within GFF577 at 309.800 kb on + strand, within GFF577 at 309.800 kb on + strand, within GFF577 at 309.801 kb on - strand, within GFF577 at 309.801 kb on - strand, within GFF577 at 309.804 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.891 kb on + strand, within GFF577 at 309.892 kb on - strand, within GFF577 at 309.898 kb on - strand, within GFF577 at 309.951 kb on + strand, within GFF577 at 309.988 kb on - strand, within GFF577 at 309.988 kb on - strand, within GFF577 at 310.038 kb on - strand, within GFF577 at 310.053 kb on + strand, within GFF577 at 310.107 kb on - strand, within GFF577 at 310.134 kb on + strand, within GFF577 at 310.135 kb on - strand, within GFF577 at 310.209 kb on + strand at 310.209 kb on + strand at 310.276 kb on + strand at 310.276 kb on + strand at 310.282 kb on + strand at 310.283 kb on - strand at 310.461 kb on - strand, within GFF578 at 310.485 kb on + strand, within GFF578 at 310.485 kb on + strand, within GFF578 at 310.486 kb on - strand, within GFF578 at 310.486 kb on - strand, within GFF578 at 310.565 kb on - strand, within GFF578 at 310.565 kb on - strand, within GFF578 at 310.566 kb on + strand, within GFF578 at 310.572 kb on + strand, within GFF578 at 310.573 kb on - strand, within GFF578 at 310.603 kb on + strand, within GFF578 at 310.758 kb on + strand, within GFF578 at 310.758 kb on + strand, within GFF578 at 310.817 kb on + strand, within GFF578 at 310.873 kb on - strand, within GFF578 at 310.875 kb on + strand, within GFF578 at 310.886 kb on + strand, within GFF578 at 310.886 kb on + strand, within GFF578 at 310.887 kb on - strand, within GFF578 at 310.888 kb on + strand, within GFF578 at 310.909 kb on - strand, within GFF578 at 310.909 kb on - strand, within GFF578 at 310.923 kb on - strand, within GFF578 at 310.996 kb on + strand, within GFF578 at 311.418 kb on + strand, within GFF578 at 311.418 kb on + strand, within GFF578 at 311.419 kb on - strand, within GFF578 at 311.419 kb on - strand, within GFF578 at 311.544 kb on - strand, within GFF578 at 311.617 kb on + strand at 311.617 kb on + strand at 311.646 kb on - strand at 311.686 kb on - strand at 311.697 kb on + strand at 311.719 kb on - strand at 311.733 kb on + strand at 311.734 kb on - strand at 311.734 kb on - strand at 311.779 kb on - strand at 311.779 kb on - strand at 311.785 kb on + strand at 311.788 kb on - strand at 311.899 kb on - strand at 311.936 kb on - strand at 311.968 kb on - strand, within GFF579 at 312.038 kb on + strand, within GFF579 at 312.175 kb on + strand, within GFF579 at 312.196 kb on - strand, within GFF579 at 312.472 kb on + strand, within GFF579 at 312.473 kb on - strand, within GFF579 at 312.561 kb on - strand, within GFF579 at 312.561 kb on - strand, within GFF579 at 312.577 kb on + strand, within GFF579
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 309,291 + GFF577 0.25 -2.2 309,351 + GFF577 0.29 +0.7 309,351 + GFF577 0.29 -0.8 309,352 - GFF577 0.29 -2.3 309,374 - GFF577 0.31 -0.3 309,400 - GFF577 0.33 +1.0 309,400 - GFF577 0.33 -1.0 309,403 + GFF577 0.33 -0.9 309,404 - GFF577 0.33 +1.5 309,424 - GFF577 0.35 +2.7 309,424 - GFF577 0.35 +0.2 309,472 - GFF577 0.39 +0.2 309,640 + GFF577 0.52 -2.3 309,641 - GFF577 0.52 -0.9 309,800 + GFF577 0.64 -0.4 309,800 + GFF577 0.64 -1.0 309,801 - GFF577 0.64 +0.6 309,801 - GFF577 0.64 -0.7 309,804 + GFF577 0.64 -1.0 309,868 + GFF577 0.69 +1.8 309,868 + GFF577 0.69 -0.1 309,868 + GFF577 0.69 -1.1 309,868 + GFF577 0.69 -0.2 309,869 - GFF577 0.69 -0.0 309,869 - GFF577 0.69 +0.1 309,869 - GFF577 0.69 -0.1 309,869 - GFF577 0.69 +0.5 309,891 + GFF577 0.71 +0.3 309,892 - GFF577 0.71 -0.8 309,898 - GFF577 0.72 -0.0 309,951 + GFF577 0.76 +2.6 309,988 - GFF577 0.78 +0.3 309,988 - GFF577 0.78 -0.1 310,038 - GFF577 0.82 -1.7 310,053 + GFF577 0.83 +1.1 310,107 - GFF577 0.88 +1.2 310,134 + GFF577 0.90 -0.3 310,135 - GFF577 0.90 -3.2 310,209 + -1.8 310,209 + -2.5 310,276 + -1.9 310,276 + -0.8 310,282 + -2.0 310,283 - -1.2 310,461 - GFF578 0.14 +0.5 310,485 + GFF578 0.15 -0.7 310,485 + GFF578 0.15 +0.9 310,486 - GFF578 0.15 -0.5 310,486 - GFF578 0.15 -2.2 310,565 - GFF578 0.21 -0.7 310,565 - GFF578 0.21 -2.0 310,566 + GFF578 0.21 -2.1 310,572 + GFF578 0.21 -2.6 310,573 - GFF578 0.21 -0.3 310,603 + GFF578 0.24 -0.4 310,758 + GFF578 0.34 +0.1 310,758 + GFF578 0.34 -0.3 310,817 + GFF578 0.38 +0.2 310,873 - GFF578 0.42 +0.3 310,875 + GFF578 0.42 -0.6 310,886 + GFF578 0.43 -0.6 310,886 + GFF578 0.43 -0.3 310,887 - GFF578 0.43 -0.3 310,888 + GFF578 0.43 -2.0 310,909 - GFF578 0.45 -1.2 310,909 - GFF578 0.45 +0.1 310,923 - GFF578 0.46 -1.5 310,996 + GFF578 0.51 -1.3 311,418 + GFF578 0.80 -0.8 311,418 + GFF578 0.80 -1.0 311,419 - GFF578 0.80 -0.2 311,419 - GFF578 0.80 +0.0 311,544 - GFF578 0.89 -0.3 311,617 + -0.2 311,617 + +1.1 311,646 - -0.1 311,686 - +0.2 311,697 + -0.3 311,719 - +0.1 311,733 + +0.3 311,734 - -0.2 311,734 - +2.1 311,779 - +0.5 311,779 - -0.7 311,785 + +0.3 311,788 - -0.6 311,899 - +0.3 311,936 - +1.2 311,968 - GFF579 0.10 -0.0 312,038 + GFF579 0.13 -0.3 312,175 + GFF579 0.19 -1.2 312,196 - GFF579 0.19 +0.3 312,472 + GFF579 0.31 +0.2 312,473 - GFF579 0.31 +0.8 312,561 - GFF579 0.34 -0.0 312,561 - GFF579 0.34 +0.5 312,577 + GFF579 0.35 +0.2
Or see this region's nucleotide sequence