Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF507 and GFF508 are separated by 325 nucleotides GFF508 and GFF509 are separated by 178 nucleotides
GFF507 - Cytoplasmic trehalase (EC 3.2.1.28), at 235,855 to 237,504
GFF507
GFF508 - Transcriptional regulator, GntR family, at 237,830 to 238,549
GFF508
GFF509 - Putative phosphosugar-binding protein, at 238,728 to 239,702
GFF509
Position (kb) 237
238
239 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 236.856 kb on - strand, within GFF507 at 236.877 kb on + strand, within GFF507 at 236.885 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.929 kb on + strand, within GFF507 at 236.967 kb on + strand, within GFF507 at 236.968 kb on - strand, within GFF507 at 236.968 kb on - strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.021 kb on - strand, within GFF507 at 237.103 kb on - strand, within GFF507 at 237.137 kb on + strand, within GFF507 at 237.219 kb on + strand, within GFF507 at 237.308 kb on - strand, within GFF507 at 237.330 kb on - strand, within GFF507 at 237.459 kb on + strand at 237.460 kb on - strand at 237.740 kb on - strand at 237.792 kb on + strand at 237.869 kb on + strand at 237.871 kb on + strand at 237.918 kb on - strand, within GFF508 at 238.110 kb on + strand, within GFF508 at 238.111 kb on - strand, within GFF508 at 238.235 kb on - strand, within GFF508 at 238.275 kb on + strand, within GFF508 at 238.322 kb on - strand, within GFF508 at 238.334 kb on + strand, within GFF508 at 238.335 kb on - strand, within GFF508 at 238.335 kb on - strand, within GFF508 at 238.335 kb on - strand, within GFF508 at 238.362 kb on + strand, within GFF508 at 238.362 kb on + strand, within GFF508 at 238.545 kb on + strand at 238.560 kb on - strand at 238.755 kb on - strand at 238.788 kb on - strand at 238.801 kb on - strand at 238.907 kb on + strand, within GFF509 at 238.968 kb on - strand, within GFF509 at 238.985 kb on + strand, within GFF509 at 239.073 kb on + strand, within GFF509 at 239.074 kb on - strand, within GFF509 at 239.108 kb on + strand, within GFF509 at 239.280 kb on + strand, within GFF509 at 239.282 kb on + strand, within GFF509 at 239.342 kb on - strand, within GFF509 at 239.390 kb on + strand, within GFF509
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 236,856 - GFF507 0.61 +0.5 236,877 + GFF507 0.62 -0.6 236,885 + GFF507 0.62 +0.2 236,925 + GFF507 0.65 +0.6 236,925 + GFF507 0.65 -0.9 236,925 + GFF507 0.65 -1.9 236,925 + GFF507 0.65 +0.7 236,925 + GFF507 0.65 -2.2 236,929 + GFF507 0.65 -2.2 236,967 + GFF507 0.67 -2.7 236,968 - GFF507 0.67 -0.4 236,968 - GFF507 0.67 -0.8 237,020 + GFF507 0.71 +1.0 237,020 + GFF507 0.71 +0.8 237,020 + GFF507 0.71 -1.6 237,020 + GFF507 0.71 +0.2 237,021 - GFF507 0.71 +1.3 237,103 - GFF507 0.76 -0.4 237,137 + GFF507 0.78 +1.5 237,219 + GFF507 0.83 -1.2 237,308 - GFF507 0.88 +1.5 237,330 - GFF507 0.89 -0.7 237,459 + -0.7 237,460 - -4.0 237,740 - -0.7 237,792 + -3.3 237,869 + +0.1 237,871 + +0.2 237,918 - GFF508 0.12 -1.2 238,110 + GFF508 0.39 -1.1 238,111 - GFF508 0.39 -1.3 238,235 - GFF508 0.56 -1.5 238,275 + GFF508 0.62 -0.5 238,322 - GFF508 0.68 -0.3 238,334 + GFF508 0.70 +0.9 238,335 - GFF508 0.70 -2.3 238,335 - GFF508 0.70 +0.4 238,335 - GFF508 0.70 +0.0 238,362 + GFF508 0.74 -0.9 238,362 + GFF508 0.74 -2.3 238,545 + -1.3 238,560 - +0.4 238,755 - +1.5 238,788 - +0.0 238,801 - -0.2 238,907 + GFF509 0.18 -1.0 238,968 - GFF509 0.25 -1.2 238,985 + GFF509 0.26 -0.1 239,073 + GFF509 0.35 +2.1 239,074 - GFF509 0.35 +0.0 239,108 + GFF509 0.39 -0.6 239,280 + GFF509 0.57 +0.5 239,282 + GFF509 0.57 +1.4 239,342 - GFF509 0.63 +0.9 239,390 + GFF509 0.68 -0.9
Or see this region's nucleotide sequence