Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4905 and GFF4906 are separated by 145 nucleotides GFF4906 and GFF4907 overlap by 4 nucleotides
GFF4905 - Macrolide-specific efflux protein MacA, at 5,347 to 6,465
GFF4905
GFF4906 - Virulence factor VirK, at 6,611 to 7,561
GFF4906
GFF4907 - Predicted ATP-dependent endonuclease of the OLD family, YbjD subgroup, at 7,558 to 9,216
GFF4907
Position (kb) 6
7
8 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.975 kb on - strand, within GFF4905 at 5.975 kb on - strand, within GFF4905 at 6.054 kb on + strand, within GFF4905 at 6.059 kb on - strand, within GFF4905 at 6.097 kb on + strand, within GFF4905 at 6.200 kb on - strand, within GFF4905 at 6.214 kb on - strand, within GFF4905 at 6.377 kb on - strand at 6.378 kb on - strand at 6.592 kb on + strand at 6.593 kb on - strand at 6.593 kb on - strand at 6.650 kb on + strand at 6.651 kb on - strand at 6.757 kb on - strand, within GFF4906 at 6.813 kb on + strand, within GFF4906 at 6.868 kb on + strand, within GFF4906 at 6.876 kb on + strand, within GFF4906 at 7.303 kb on + strand, within GFF4906 at 7.303 kb on + strand, within GFF4906 at 7.336 kb on + strand, within GFF4906 at 7.337 kb on - strand, within GFF4906 at 7.427 kb on + strand, within GFF4906 at 7.427 kb on + strand, within GFF4906 at 7.432 kb on + strand, within GFF4906 at 7.480 kb on + strand at 7.788 kb on + strand, within GFF4907 at 7.989 kb on + strand, within GFF4907 at 8.037 kb on + strand, within GFF4907 at 8.321 kb on - strand, within GFF4907 at 8.367 kb on + strand, within GFF4907 at 8.491 kb on - strand, within GFF4907 at 8.561 kb on + strand, within GFF4907
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 5,974 + GFF4905 0.56 +0.3 5,974 + GFF4905 0.56 -1.3 5,974 + GFF4905 0.56 +1.2 5,974 + GFF4905 0.56 +0.9 5,975 - GFF4905 0.56 -0.4 5,975 - GFF4905 0.56 +0.1 6,054 + GFF4905 0.63 +0.3 6,059 - GFF4905 0.64 -1.0 6,097 + GFF4905 0.67 +0.4 6,200 - GFF4905 0.76 +0.1 6,214 - GFF4905 0.77 -0.4 6,377 - -2.4 6,378 - -1.4 6,592 + -1.0 6,593 - +0.8 6,593 - +0.4 6,650 + +0.2 6,651 - -1.9 6,757 - GFF4906 0.15 +0.9 6,813 + GFF4906 0.21 +0.3 6,868 + GFF4906 0.27 -0.6 6,876 + GFF4906 0.28 -0.2 7,303 + GFF4906 0.73 +0.1 7,303 + GFF4906 0.73 -0.7 7,336 + GFF4906 0.76 -0.3 7,337 - GFF4906 0.76 +2.1 7,427 + GFF4906 0.86 -0.0 7,427 + GFF4906 0.86 +0.3 7,432 + GFF4906 0.86 +2.9 7,480 + -0.6 7,788 + GFF4907 0.14 -3.0 7,989 + GFF4907 0.26 -0.9 8,037 + GFF4907 0.29 +0.3 8,321 - GFF4907 0.46 -0.9 8,367 + GFF4907 0.49 +0.5 8,491 - GFF4907 0.56 +0.3 8,561 + GFF4907 0.60 +0.1
Or see this region's nucleotide sequence