Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4788 and GFF4789 are separated by 62 nucleotides GFF4789 and GFF4790 are separated by 13 nucleotides GFF4790 and GFF4791 are separated by 138 nucleotides GFF4791 and GFF4792 overlap by 7 nucleotides
GFF4788 - RNA polymerase sigma factor RpoS, at 95,277 to 96,269
GFF4788
GFF4789 - Lipoprotein NlpD, at 96,332 to 97,291
GFF4789
GFF4790 - FIG01047833: hypothetical protein, at 97,305 to 97,502
GFF4790
GFF4791 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 97,641 to 98,267
GFF4791
GFF4792 - 5-nucleotidase SurE (EC 3.1.3.5) @ Exopolyphosphatase (EC 3.6.1.11), at 98,261 to 99,013
GFF4792
Position (kb)
96
97
98 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 95.605 kb on - strand, within GFF4788 at 95.637 kb on - strand, within GFF4788 at 95.637 kb on - strand, within GFF4788 at 95.709 kb on - strand, within GFF4788 at 95.740 kb on - strand, within GFF4788 at 95.740 kb on - strand, within GFF4788 at 95.740 kb on - strand, within GFF4788 at 95.837 kb on - strand, within GFF4788 at 96.282 kb on - strand at 96.404 kb on - strand at 96.482 kb on - strand, within GFF4789 at 96.482 kb on - strand, within GFF4789 at 96.530 kb on - strand, within GFF4789 at 96.539 kb on - strand, within GFF4789 at 96.544 kb on - strand, within GFF4789 at 96.567 kb on + strand, within GFF4789 at 96.569 kb on + strand, within GFF4789 at 96.846 kb on + strand, within GFF4789 at 96.846 kb on + strand, within GFF4789 at 96.850 kb on + strand, within GFF4789 at 96.851 kb on - strand, within GFF4789 at 97.022 kb on - strand, within GFF4789 at 97.105 kb on - strand, within GFF4789 at 97.105 kb on - strand, within GFF4789 at 97.108 kb on + strand, within GFF4789 at 97.122 kb on + strand, within GFF4789 at 97.123 kb on - strand, within GFF4789 at 97.126 kb on + strand, within GFF4789 at 97.386 kb on + strand, within GFF4790 at 97.387 kb on + strand, within GFF4790 at 97.524 kb on + strand at 97.617 kb on - strand at 97.617 kb on - strand at 97.643 kb on - strand at 97.663 kb on - strand at 97.802 kb on + strand, within GFF4791 at 97.902 kb on + strand, within GFF4791 at 97.903 kb on - strand, within GFF4791 at 98.081 kb on + strand, within GFF4791 at 98.082 kb on - strand, within GFF4791 at 98.083 kb on + strand, within GFF4791 at 98.083 kb on + strand, within GFF4791 at 98.084 kb on - strand, within GFF4791 at 98.084 kb on - strand, within GFF4791 at 98.127 kb on - strand, within GFF4791 at 98.142 kb on - strand, within GFF4791 at 98.162 kb on + strand, within GFF4791 at 98.250 kb on + strand at 98.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 95,605 - GFF4788 0.33 -0.5 95,637 - GFF4788 0.36 -0.6 95,637 - GFF4788 0.36 +1.1 95,709 - GFF4788 0.44 +2.4 95,740 - GFF4788 0.47 +1.7 95,740 - GFF4788 0.47 +1.7 95,740 - GFF4788 0.47 +1.9 95,837 - GFF4788 0.56 +0.9 96,282 - +1.1 96,404 - +1.7 96,482 - GFF4789 0.16 +0.3 96,482 - GFF4789 0.16 +3.2 96,530 - GFF4789 0.21 -2.6 96,539 - GFF4789 0.22 +1.3 96,544 - GFF4789 0.22 +0.9 96,567 + GFF4789 0.24 +3.7 96,569 + GFF4789 0.25 +0.5 96,846 + GFF4789 0.54 +1.6 96,846 + GFF4789 0.54 +4.1 96,850 + GFF4789 0.54 +1.6 96,851 - GFF4789 0.54 -2.2 97,022 - GFF4789 0.72 +1.5 97,105 - GFF4789 0.81 +1.2 97,105 - GFF4789 0.81 +0.0 97,108 + GFF4789 0.81 +2.6 97,122 + GFF4789 0.82 -2.7 97,123 - GFF4789 0.82 +1.8 97,126 + GFF4789 0.83 -0.2 97,386 + GFF4790 0.41 -0.4 97,387 + GFF4790 0.41 +0.2 97,524 + +0.0 97,617 - +2.0 97,617 - -1.0 97,643 - +0.8 97,663 - -0.9 97,802 + GFF4791 0.26 -0.2 97,902 + GFF4791 0.42 -0.6 97,903 - GFF4791 0.42 +0.8 98,081 + GFF4791 0.70 +0.2 98,082 - GFF4791 0.70 -0.7 98,083 + GFF4791 0.70 -0.5 98,083 + GFF4791 0.70 -0.4 98,084 - GFF4791 0.71 +0.1 98,084 - GFF4791 0.71 -1.3 98,127 - GFF4791 0.78 -0.7 98,142 - GFF4791 0.80 -3.1 98,162 + GFF4791 0.83 -0.5 98,250 + +0.3 98,251 - +0.9
Or see this region's nucleotide sequence