Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4696 and GFF4697 overlap by 4 nucleotides GFF4697 and GFF4698 are separated by 11 nucleotides GFF4698 and GFF4699 are separated by 11 nucleotides GFF4699 and GFF4700 are separated by 75 nucleotides
GFF4696 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 8,088 to 8,651
GFF4696
GFF4697 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 8,648 to 9,619
GFF4697
GFF4698 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 9,631 to 9,993
GFF4698
GFF4699 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 10,005 to 10,784
GFF4699
GFF4700 - Glucitol operon activator protein, at 10,860 to 11,219
GFF4700
Position (kb)
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 8.711 kb on - strand at 8.732 kb on + strand at 8.732 kb on + strand at 8.734 kb on + strand at 8.734 kb on + strand at 8.735 kb on - strand at 8.735 kb on - strand at 8.781 kb on + strand, within GFF4697 at 8.951 kb on + strand, within GFF4697 at 8.954 kb on - strand, within GFF4697 at 8.970 kb on - strand, within GFF4697 at 8.998 kb on + strand, within GFF4697 at 9.142 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.579 kb on + strand at 9.580 kb on - strand at 9.608 kb on - strand at 9.618 kb on - strand at 9.709 kb on - strand, within GFF4698 at 9.709 kb on - strand, within GFF4698 at 9.880 kb on - strand, within GFF4698 at 9.929 kb on + strand, within GFF4698 at 10.104 kb on - strand, within GFF4699 at 10.264 kb on - strand, within GFF4699 at 10.428 kb on - strand, within GFF4699 at 10.540 kb on + strand, within GFF4699 at 10.645 kb on - strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.714 kb on - strand at 10.859 kb on + strand at 10.860 kb on - strand at 10.961 kb on + strand, within GFF4700
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 8,711 - +0.1 8,732 + -0.3 8,732 + +0.7 8,734 + -1.3 8,734 + -0.6 8,735 - -2.7 8,735 - -0.8 8,781 + GFF4697 0.14 -3.3 8,951 + GFF4697 0.31 +0.6 8,954 - GFF4697 0.31 +0.8 8,970 - GFF4697 0.33 -0.1 8,998 + GFF4697 0.36 -0.2 9,142 - GFF4697 0.51 +0.1 9,233 - GFF4697 0.60 +0.8 9,233 - GFF4697 0.60 +1.6 9,579 + +0.8 9,580 - +0.3 9,608 - +1.1 9,618 - -0.4 9,709 - GFF4698 0.21 -0.1 9,709 - GFF4698 0.21 -0.7 9,880 - GFF4698 0.69 -1.3 9,929 + GFF4698 0.82 -1.3 10,104 - GFF4699 0.13 -3.0 10,264 - GFF4699 0.33 -1.2 10,428 - GFF4699 0.54 +1.5 10,540 + GFF4699 0.69 -0.3 10,645 - GFF4699 0.82 -1.3 10,647 + GFF4699 0.82 +1.0 10,647 + GFF4699 0.82 +1.4 10,648 - GFF4699 0.82 +1.1 10,648 - GFF4699 0.82 -1.0 10,714 - +0.8 10,859 + -2.2 10,860 - +0.5 10,961 + GFF4700 0.28 -2.2
Or see this region's nucleotide sequence