Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4683 and GFF4684 are separated by 98 nucleotides GFF4684 and GFF4685 are separated by 116 nucleotides GFF4685 and GFF4686 are separated by 89 nucleotides GFF4686 and GFF4687 are separated by 9 nucleotides
GFF4683 - Phage minor tail protein, at 13,307 to 13,639
GFF4683
GFF4684 - Attachment invasion locus protein precursor, at 13,738 to 14,235
GFF4684
GFF4685 - Superoxide dismutase [Cu-Zn] precursor (EC 1.15.1.1), at 14,352 to 14,885
GFF4685
GFF4686 - Phage minor tail protein, at 14,975 to 15,670
GFF4686
GFF4687 - Phage tail assembly protein, at 15,680 to 16,417
GFF4687
Position (kb) 14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 13.544 kb on + strand, within GFF4683 at 13.555 kb on + strand, within GFF4683 at 13.666 kb on + strand at 13.782 kb on + strand at 13.840 kb on + strand, within GFF4684 at 13.941 kb on + strand, within GFF4684 at 13.942 kb on - strand, within GFF4684 at 13.945 kb on + strand, within GFF4684 at 13.946 kb on - strand, within GFF4684 at 13.965 kb on + strand, within GFF4684 at 13.969 kb on + strand, within GFF4684 at 13.976 kb on - strand, within GFF4684 at 13.977 kb on + strand, within GFF4684 at 13.997 kb on - strand, within GFF4684 at 13.998 kb on + strand, within GFF4684 at 14.039 kb on - strand, within GFF4684 at 14.088 kb on + strand, within GFF4684 at 14.187 kb on + strand at 14.343 kb on - strand at 14.343 kb on - strand at 14.565 kb on - strand, within GFF4685 at 14.566 kb on + strand, within GFF4685 at 14.636 kb on + strand, within GFF4685 at 14.808 kb on + strand, within GFF4685 at 14.871 kb on + strand at 14.891 kb on - strand at 14.939 kb on - strand at 14.972 kb on + strand at 14.974 kb on + strand at 14.974 kb on + strand at 14.974 kb on + strand at 14.985 kb on + strand at 15.300 kb on - strand, within GFF4686 at 15.300 kb on - strand, within GFF4686 at 15.380 kb on - strand, within GFF4686 at 15.418 kb on + strand, within GFF4686 at 15.424 kb on + strand, within GFF4686 at 15.472 kb on + strand, within GFF4686 at 15.619 kb on + strand at 15.668 kb on + strand at 15.817 kb on + strand, within GFF4687 at 15.818 kb on - strand, within GFF4687 at 15.818 kb on - strand, within GFF4687
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 13,544 + GFF4683 0.71 +1.3 13,555 + GFF4683 0.74 -0.3 13,666 + +0.5 13,782 + +0.6 13,840 + GFF4684 0.20 +0.0 13,941 + GFF4684 0.41 +0.5 13,942 - GFF4684 0.41 -0.6 13,945 + GFF4684 0.42 -0.7 13,946 - GFF4684 0.42 -0.7 13,965 + GFF4684 0.46 -0.4 13,969 + GFF4684 0.46 -0.7 13,976 - GFF4684 0.48 +0.3 13,977 + GFF4684 0.48 +0.3 13,997 - GFF4684 0.52 +0.1 13,998 + GFF4684 0.52 -1.5 14,039 - GFF4684 0.60 -0.3 14,088 + GFF4684 0.70 -0.4 14,187 + +0.9 14,343 - +0.2 14,343 - +0.8 14,565 - GFF4685 0.40 -0.3 14,566 + GFF4685 0.40 -0.4 14,636 + GFF4685 0.53 -0.5 14,808 + GFF4685 0.85 +0.2 14,871 + -1.1 14,891 - -0.4 14,939 - +0.2 14,972 + -0.6 14,974 + -3.2 14,974 + +0.0 14,974 + -0.1 14,985 + +0.3 15,300 - GFF4686 0.47 +0.3 15,300 - GFF4686 0.47 -0.8 15,380 - GFF4686 0.58 -1.9 15,418 + GFF4686 0.64 -2.3 15,424 + GFF4686 0.65 +0.3 15,472 + GFF4686 0.71 -0.3 15,619 + -0.2 15,668 + +1.1 15,817 + GFF4687 0.19 -0.1 15,818 - GFF4687 0.19 -1.3 15,818 - GFF4687 0.19 -2.6
Or see this region's nucleotide sequence