Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF458 and GFF459 are separated by 218 nucleotides GFF459 and GFF460 are separated by 51 nucleotides GFF460 and GFF461 overlap by 16 nucleotides
GFF458 - 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 178,417 to 179,391
GFF458
GFF459 - Outer membrane protein A precursor, at 179,610 to 180,272
GFF459
GFF460 - hypothetical protein, at 180,324 to 180,440
GFF460
GFF461 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.5.3), at 180,425 to 182,758
GFF461
Position (kb) 179
180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 178.628 kb on - strand, within GFF458 at 178.628 kb on - strand, within GFF458 at 178.695 kb on + strand, within GFF458 at 178.714 kb on - strand, within GFF458 at 178.806 kb on + strand, within GFF458 at 178.806 kb on + strand, within GFF458 at 178.821 kb on - strand, within GFF458 at 178.886 kb on + strand, within GFF458 at 178.888 kb on - strand, within GFF458 at 179.136 kb on + strand, within GFF458 at 179.157 kb on + strand, within GFF458 at 179.446 kb on + strand at 179.504 kb on - strand at 179.511 kb on + strand at 179.566 kb on - strand at 179.606 kb on + strand at 179.611 kb on + strand at 179.611 kb on + strand at 179.657 kb on + strand at 179.657 kb on + strand at 179.936 kb on + strand, within GFF459 at 179.957 kb on + strand, within GFF459 at 179.957 kb on + strand, within GFF459 at 179.958 kb on - strand, within GFF459 at 179.958 kb on - strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.035 kb on - strand, within GFF459 at 180.035 kb on - strand, within GFF459 at 180.092 kb on + strand, within GFF459 at 180.174 kb on - strand, within GFF459 at 180.219 kb on + strand at 180.220 kb on - strand at 180.259 kb on - strand at 180.259 kb on - strand at 180.284 kb on - strand at 180.378 kb on - strand, within GFF460 at 180.583 kb on + strand at 180.614 kb on - strand at 180.652 kb on - strand at 180.652 kb on - strand at 180.737 kb on + strand, within GFF461 at 180.767 kb on + strand, within GFF461 at 180.789 kb on + strand, within GFF461 at 180.805 kb on + strand, within GFF461 at 180.821 kb on + strand, within GFF461 at 180.842 kb on + strand, within GFF461 at 180.842 kb on + strand, within GFF461 at 180.842 kb on + strand, within GFF461 at 180.886 kb on + strand, within GFF461 at 180.896 kb on + strand, within GFF461 at 180.897 kb on - strand, within GFF461 at 180.920 kb on + strand, within GFF461
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 178,628 - GFF458 0.22 -0.5 178,628 - GFF458 0.22 -0.4 178,695 + GFF458 0.29 +1.5 178,714 - GFF458 0.30 -1.6 178,806 + GFF458 0.40 +1.3 178,806 + GFF458 0.40 +0.4 178,821 - GFF458 0.41 +0.1 178,886 + GFF458 0.48 +0.1 178,888 - GFF458 0.48 +0.1 179,136 + GFF458 0.74 -2.0 179,157 + GFF458 0.76 -0.3 179,446 + +0.2 179,504 - -0.4 179,511 + +0.1 179,566 - +1.9 179,606 + +0.5 179,611 + +0.8 179,611 + -0.4 179,657 + -3.0 179,657 + -0.8 179,936 + GFF459 0.49 +0.9 179,957 + GFF459 0.52 +0.3 179,957 + GFF459 0.52 -0.4 179,958 - GFF459 0.52 +1.1 179,958 - GFF459 0.52 +0.1 180,034 + GFF459 0.64 -1.8 180,034 + GFF459 0.64 -0.0 180,034 + GFF459 0.64 +0.5 180,034 + GFF459 0.64 -2.7 180,035 - GFF459 0.64 +0.7 180,035 - GFF459 0.64 +0.2 180,092 + GFF459 0.73 +0.4 180,174 - GFF459 0.85 -1.4 180,219 + +0.2 180,220 - +0.1 180,259 - +1.0 180,259 - -0.5 180,284 - +0.3 180,378 - GFF460 0.46 +0.0 180,583 + +1.1 180,614 - +0.3 180,652 - +0.4 180,652 - +2.6 180,737 + GFF461 0.13 +0.0 180,767 + GFF461 0.15 +0.2 180,789 + GFF461 0.16 +1.4 180,805 + GFF461 0.16 +0.3 180,821 + GFF461 0.17 +0.2 180,842 + GFF461 0.18 -1.9 180,842 + GFF461 0.18 +0.4 180,842 + GFF461 0.18 -0.3 180,886 + GFF461 0.20 +0.2 180,896 + GFF461 0.20 -0.4 180,897 - GFF461 0.20 -0.6 180,920 + GFF461 0.21 -1.5
Or see this region's nucleotide sequence