Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4569 and GFF4570 are separated by 116 nucleotides GFF4570 and GFF4571 overlap by 1 nucleotides GFF4571 and GFF4572 overlap by 1 nucleotides GFF4572 and GFF4573 are separated by 159 nucleotides
GFF4569 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 355,772 to 356,707
GFF4569
GFF4570 - Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4), at 356,824 to 357,549
GFF4570
GFF4571 - Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4), at 357,549 to 358,223
GFF4571
GFF4572 - Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4), at 358,223 to 358,963
GFF4572
GFF4573 - Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4), at 359,123 to 360,031
GFF4573
Position (kb) 357
358
359 Strain fitness (log2 ratio) -2
-1
0
1
2 at 356.690 kb on - strand at 356.720 kb on + strand at 356.728 kb on - strand at 356.780 kb on + strand at 356.913 kb on + strand, within GFF4570 at 357.017 kb on - strand, within GFF4570 at 357.074 kb on - strand, within GFF4570 at 357.093 kb on + strand, within GFF4570 at 357.093 kb on + strand, within GFF4570 at 357.094 kb on - strand, within GFF4570 at 357.094 kb on - strand, within GFF4570 at 357.094 kb on - strand, within GFF4570 at 357.305 kb on - strand, within GFF4570 at 357.461 kb on - strand, within GFF4570 at 357.571 kb on + strand at 357.611 kb on - strand at 357.730 kb on - strand, within GFF4571 at 357.789 kb on - strand, within GFF4571 at 358.049 kb on - strand, within GFF4571 at 358.180 kb on + strand at 358.288 kb on + strand at 358.308 kb on + strand, within GFF4572 at 358.308 kb on + strand, within GFF4572 at 358.309 kb on - strand, within GFF4572 at 358.498 kb on - strand, within GFF4572 at 358.744 kb on + strand, within GFF4572 at 358.744 kb on + strand, within GFF4572 at 358.757 kb on + strand, within GFF4572 at 358.761 kb on + strand, within GFF4572 at 358.806 kb on - strand, within GFF4572 at 358.819 kb on + strand, within GFF4572 at 358.903 kb on - strand at 358.955 kb on + strand at 358.963 kb on + strand at 358.963 kb on + strand at 358.963 kb on + strand at 358.964 kb on - strand at 358.964 kb on - strand at 359.061 kb on + strand at 359.061 kb on + strand at 359.061 kb on + strand at 359.063 kb on + strand at 359.063 kb on + strand at 359.064 kb on - strand at 359.064 kb on - strand at 359.137 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 356,690 - +0.5 356,720 + -0.1 356,728 - -0.1 356,780 + -0.9 356,913 + GFF4570 0.12 +0.2 357,017 - GFF4570 0.27 -0.1 357,074 - GFF4570 0.34 +0.7 357,093 + GFF4570 0.37 +0.3 357,093 + GFF4570 0.37 -0.3 357,094 - GFF4570 0.37 +0.2 357,094 - GFF4570 0.37 -1.3 357,094 - GFF4570 0.37 +1.9 357,305 - GFF4570 0.66 +0.1 357,461 - GFF4570 0.88 +0.1 357,571 + -0.5 357,611 - +0.0 357,730 - GFF4571 0.27 +0.3 357,789 - GFF4571 0.36 -1.2 358,049 - GFF4571 0.74 -1.0 358,180 + +0.9 358,288 + -0.9 358,308 + GFF4572 0.11 -0.1 358,308 + GFF4572 0.11 +0.6 358,309 - GFF4572 0.12 +0.4 358,498 - GFF4572 0.37 -1.1 358,744 + GFF4572 0.70 -0.1 358,744 + GFF4572 0.70 -0.2 358,757 + GFF4572 0.72 -0.7 358,761 + GFF4572 0.73 -0.0 358,806 - GFF4572 0.79 -0.0 358,819 + GFF4572 0.80 +0.5 358,903 - +2.0 358,955 + -0.5 358,963 + +0.3 358,963 + -1.8 358,963 + -0.1 358,964 - -0.4 358,964 - +0.1 359,061 + +1.2 359,061 + +1.1 359,061 + +0.0 359,063 + -1.9 359,063 + -0.5 359,064 - -0.1 359,064 - +1.0 359,137 + +0.9
Or see this region's nucleotide sequence