Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4560 and GFF4561 are separated by 92 nucleotides GFF4561 and GFF4562 are separated by 38 nucleotides
GFF4560 - Propionate catabolism operon regulatory protein PrpR, at 344,759 to 346,687
GFF4560
GFF4561 - FIG002095: hypothetical protein, at 346,780 to 347,253
GFF4561
GFF4562 - TETRATRICOPEPTIDE REPEAT FAMILY PROTEIN, at 347,292 to 348,287
GFF4562
Position (kb) 346
347
348 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 345.825 kb on + strand, within GFF4560 at 345.826 kb on - strand, within GFF4560 at 345.872 kb on - strand, within GFF4560 at 345.965 kb on + strand, within GFF4560 at 346.089 kb on + strand, within GFF4560 at 346.143 kb on + strand, within GFF4560 at 346.144 kb on - strand, within GFF4560 at 346.144 kb on - strand, within GFF4560 at 346.226 kb on + strand, within GFF4560 at 346.227 kb on - strand, within GFF4560 at 346.243 kb on - strand, within GFF4560 at 346.243 kb on - strand, within GFF4560 at 346.312 kb on + strand, within GFF4560 at 346.312 kb on + strand, within GFF4560 at 346.313 kb on - strand, within GFF4560 at 346.316 kb on - strand, within GFF4560 at 346.316 kb on - strand, within GFF4560 at 346.331 kb on + strand, within GFF4560 at 346.333 kb on + strand, within GFF4560 at 346.333 kb on + strand, within GFF4560 at 346.424 kb on - strand, within GFF4560 at 346.444 kb on + strand, within GFF4560 at 346.525 kb on + strand at 346.526 kb on - strand at 346.526 kb on - strand at 346.638 kb on + strand at 346.639 kb on - strand at 346.671 kb on + strand at 346.743 kb on - strand at 346.745 kb on + strand at 346.745 kb on + strand at 346.801 kb on + strand at 346.824 kb on - strand at 347.110 kb on + strand, within GFF4561 at 347.110 kb on + strand, within GFF4561 at 347.165 kb on - strand, within GFF4561 at 347.251 kb on + strand at 347.252 kb on - strand at 347.294 kb on - strand at 347.294 kb on - strand at 347.374 kb on + strand at 347.389 kb on - strand at 347.389 kb on - strand at 347.419 kb on + strand, within GFF4562 at 347.437 kb on + strand, within GFF4562 at 347.548 kb on + strand, within GFF4562 at 347.591 kb on - strand, within GFF4562 at 347.606 kb on - strand, within GFF4562 at 347.632 kb on + strand, within GFF4562 at 347.636 kb on - strand, within GFF4562 at 347.747 kb on + strand, within GFF4562 at 347.747 kb on + strand, within GFF4562 at 347.747 kb on + strand, within GFF4562 at 347.747 kb on + strand, within GFF4562 at 347.747 kb on + strand, within GFF4562 at 347.748 kb on - strand, within GFF4562 at 347.750 kb on - strand, within GFF4562 at 347.750 kb on - strand, within GFF4562 at 347.784 kb on + strand, within GFF4562 at 347.785 kb on - strand, within GFF4562 at 347.869 kb on + strand, within GFF4562 at 347.929 kb on + strand, within GFF4562 at 347.971 kb on - strand, within GFF4562
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 345,825 + GFF4560 0.55 +0.5 345,826 - GFF4560 0.55 -0.0 345,872 - GFF4560 0.58 -0.5 345,965 + GFF4560 0.63 +0.1 346,089 + GFF4560 0.69 +0.9 346,143 + GFF4560 0.72 +0.1 346,144 - GFF4560 0.72 +0.1 346,144 - GFF4560 0.72 -0.3 346,226 + GFF4560 0.76 +0.3 346,227 - GFF4560 0.76 +0.2 346,243 - GFF4560 0.77 +1.4 346,243 - GFF4560 0.77 -0.5 346,312 + GFF4560 0.81 +1.0 346,312 + GFF4560 0.81 +0.9 346,313 - GFF4560 0.81 +0.7 346,316 - GFF4560 0.81 +0.5 346,316 - GFF4560 0.81 -2.9 346,331 + GFF4560 0.81 -0.4 346,333 + GFF4560 0.82 -3.0 346,333 + GFF4560 0.82 +0.1 346,424 - GFF4560 0.86 +0.1 346,444 + GFF4560 0.87 -1.0 346,525 + -0.4 346,526 - -0.5 346,526 - +0.0 346,638 + +1.1 346,639 - +0.2 346,671 + -3.7 346,743 - -0.1 346,745 + -1.6 346,745 + -0.2 346,801 + +0.8 346,824 - -0.2 347,110 + GFF4561 0.70 -0.4 347,110 + GFF4561 0.70 -0.3 347,165 - GFF4561 0.81 -0.1 347,251 + +0.6 347,252 - -1.0 347,294 - +0.1 347,294 - +0.3 347,374 + -0.5 347,389 - +3.6 347,389 - -0.6 347,419 + GFF4562 0.13 +1.1 347,437 + GFF4562 0.15 +0.5 347,548 + GFF4562 0.26 +0.5 347,591 - GFF4562 0.30 +1.4 347,606 - GFF4562 0.32 -0.3 347,632 + GFF4562 0.34 -0.1 347,636 - GFF4562 0.35 -0.7 347,747 + GFF4562 0.46 +0.8 347,747 + GFF4562 0.46 +1.0 347,747 + GFF4562 0.46 +0.9 347,747 + GFF4562 0.46 -1.7 347,747 + GFF4562 0.46 +2.0 347,748 - GFF4562 0.46 +0.6 347,750 - GFF4562 0.46 +3.3 347,750 - GFF4562 0.46 -0.0 347,784 + GFF4562 0.49 +0.3 347,785 - GFF4562 0.49 -1.5 347,869 + GFF4562 0.58 +0.9 347,929 + GFF4562 0.64 -1.6 347,971 - GFF4562 0.68 +0.6
Or see this region's nucleotide sequence