Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4484 and GFF4485 are separated by 288 nucleotides GFF4485 and GFF4486 are separated by 5 nucleotides GFF4486 and GFF4487 are separated by 90 nucleotides GFF4487 and GFF4488 are separated by 11 nucleotides GFF4488 and GFF4489 are separated by 45 nucleotides
GFF4484 - Transcriptional regulator, TetR family, at 268,360 to 268,941
GFF4484
GFF4485 - Transcriptional activator RamA, at 269,230 to 269,571
GFF4485
GFF4486 - Putative inner membrane protein, at 269,577 to 269,801
GFF4486
GFF4487 - FIG00638355: hypothetical protein, at 269,892 to 271,010
GFF4487
GFF4488 - '4'-phosphopantetheinyl transferase (EC 2.7.8.-) [enterobactin] siderophore' transl_table=11, at 271,022 to 271,726
GFF4488
GFF4489 - TonB-dependent receptor; Outer membrane receptor for ferric enterobactin and colicins B, D, at 271,772 to 274,027
GFF4489
Position (kb) 269
270
271
272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 269.040 kb on - strand at 269.101 kb on + strand at 269.157 kb on - strand at 269.166 kb on - strand at 269.281 kb on - strand, within GFF4485 at 269.298 kb on - strand, within GFF4485 at 269.311 kb on - strand, within GFF4485 at 269.358 kb on + strand, within GFF4485 at 269.365 kb on + strand, within GFF4485 at 269.366 kb on - strand, within GFF4485 at 269.408 kb on + strand, within GFF4485 at 269.409 kb on - strand, within GFF4485 at 269.457 kb on + strand, within GFF4485 at 269.457 kb on + strand, within GFF4485 at 269.884 kb on + strand at 269.884 kb on + strand at 269.885 kb on - strand at 269.934 kb on + strand at 269.972 kb on - strand at 270.080 kb on + strand, within GFF4487 at 270.081 kb on - strand, within GFF4487 at 270.081 kb on - strand, within GFF4487 at 270.112 kb on + strand, within GFF4487 at 270.171 kb on - strand, within GFF4487 at 270.255 kb on - strand, within GFF4487 at 270.303 kb on + strand, within GFF4487 at 270.373 kb on + strand, within GFF4487 at 270.501 kb on - strand, within GFF4487 at 270.502 kb on + strand, within GFF4487 at 270.527 kb on + strand, within GFF4487 at 270.634 kb on - strand, within GFF4487 at 270.634 kb on - strand, within GFF4487 at 270.637 kb on + strand, within GFF4487 at 270.674 kb on + strand, within GFF4487 at 270.777 kb on - strand, within GFF4487 at 270.840 kb on - strand, within GFF4487 at 270.851 kb on + strand, within GFF4487 at 270.852 kb on - strand, within GFF4487 at 270.922 kb on - strand at 270.928 kb on + strand at 270.974 kb on + strand at 270.975 kb on - strand at 270.975 kb on - strand at 271.043 kb on + strand at 271.043 kb on + strand at 271.044 kb on - strand at 271.044 kb on - strand at 271.045 kb on + strand at 271.099 kb on + strand, within GFF4488 at 271.102 kb on + strand, within GFF4488 at 271.103 kb on - strand, within GFF4488 at 271.108 kb on + strand, within GFF4488 at 271.141 kb on + strand, within GFF4488 at 271.142 kb on - strand, within GFF4488 at 271.182 kb on - strand, within GFF4488 at 271.261 kb on + strand, within GFF4488 at 271.355 kb on - strand, within GFF4488 at 271.544 kb on + strand, within GFF4488 at 271.591 kb on - strand, within GFF4488 at 271.629 kb on - strand, within GFF4488 at 271.786 kb on + strand at 271.786 kb on + strand at 271.787 kb on - strand at 271.796 kb on - strand at 271.797 kb on + strand at 271.797 kb on + strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.821 kb on + strand at 271.821 kb on + strand at 271.822 kb on - strand at 271.822 kb on - strand at 271.846 kb on + strand at 271.848 kb on + strand at 271.849 kb on - strand at 271.918 kb on + strand at 271.981 kb on + strand at 271.982 kb on - strand at 271.987 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 269,040 - +1.6 269,101 + +0.4 269,157 - +0.3 269,166 - -0.4 269,281 - GFF4485 0.15 -0.1 269,298 - GFF4485 0.20 -0.9 269,311 - GFF4485 0.24 -2.2 269,358 + GFF4485 0.37 +0.3 269,365 + GFF4485 0.39 +1.3 269,366 - GFF4485 0.40 -3.3 269,408 + GFF4485 0.52 -1.8 269,409 - GFF4485 0.52 +0.0 269,457 + GFF4485 0.66 -0.5 269,457 + GFF4485 0.66 -0.3 269,884 + +0.6 269,884 + +0.4 269,885 - +0.5 269,934 + -0.4 269,972 - -0.6 270,080 + GFF4487 0.17 -2.1 270,081 - GFF4487 0.17 -0.8 270,081 - GFF4487 0.17 +0.8 270,112 + GFF4487 0.20 +1.5 270,171 - GFF4487 0.25 -0.3 270,255 - GFF4487 0.32 -1.9 270,303 + GFF4487 0.37 +0.5 270,373 + GFF4487 0.43 +0.6 270,501 - GFF4487 0.54 +0.8 270,502 + GFF4487 0.55 -0.9 270,527 + GFF4487 0.57 -0.8 270,634 - GFF4487 0.66 +0.2 270,634 - GFF4487 0.66 -0.5 270,637 + GFF4487 0.67 -1.2 270,674 + GFF4487 0.70 +0.1 270,777 - GFF4487 0.79 +0.2 270,840 - GFF4487 0.85 -1.8 270,851 + GFF4487 0.86 +0.1 270,852 - GFF4487 0.86 -1.0 270,922 - -1.7 270,928 + -0.3 270,974 + +0.3 270,975 - -0.4 270,975 - +1.1 271,043 + +0.1 271,043 + +3.2 271,044 - +2.3 271,044 - +0.7 271,045 + +0.8 271,099 + GFF4488 0.11 -0.1 271,102 + GFF4488 0.11 +0.3 271,103 - GFF4488 0.11 +1.4 271,108 + GFF4488 0.12 -0.3 271,141 + GFF4488 0.17 -2.0 271,142 - GFF4488 0.17 -2.6 271,182 - GFF4488 0.23 +0.1 271,261 + GFF4488 0.34 +0.6 271,355 - GFF4488 0.47 -0.6 271,544 + GFF4488 0.74 -1.0 271,591 - GFF4488 0.81 -0.5 271,629 - GFF4488 0.86 +0.2 271,786 + -1.9 271,786 + +0.3 271,787 - +1.2 271,796 - -0.6 271,797 + -0.5 271,797 + -2.0 271,805 - +0.5 271,805 - -1.2 271,805 - +0.1 271,805 - +0.1 271,805 - +0.7 271,821 + +0.4 271,821 + +0.2 271,822 - +0.2 271,822 - -0.2 271,846 + -1.3 271,848 + -0.4 271,849 - -0.4 271,918 + +0.4 271,981 + -0.4 271,982 - +0.1 271,987 - -0.0
Or see this region's nucleotide sequence