Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4424

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4423 and GFF4424 are separated by 12 nucleotidesGFF4424 and GFF4425 are separated by 100 nucleotides GFF4423 - Glyoxylate carboligase (EC 4.1.1.47), at 207,681 to 209,462 GFF4423 GFF4424 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 209,475 to 210,251 GFF4424 GFF4425 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 210,352 to 211,230 GFF4425 Position (kb) 209 210 211Strain fitness (log2 ratio) -2 -1 0 1 2at 208.628 kb on - strand, within GFF4423at 208.746 kb on - strand, within GFF4423at 208.763 kb on + strand, within GFF4423at 208.846 kb on - strand, within GFF4423at 209.076 kb on + strand, within GFF4423at 209.076 kb on + strand, within GFF4423at 209.078 kb on + strand, within GFF4423at 209.104 kb on - strand, within GFF4423at 209.148 kb on + strand, within GFF4423at 209.156 kb on - strand, within GFF4423at 209.175 kb on - strand, within GFF4423at 209.215 kb on + strand, within GFF4423at 209.215 kb on + strand, within GFF4423at 209.270 kb on + strand, within GFF4423at 209.454 kb on + strandat 209.898 kb on - strand, within GFF4424at 209.988 kb on - strand, within GFF4424at 210.008 kb on + strand, within GFF4424at 210.009 kb on - strand, within GFF4424at 210.195 kb on + strandat 210.337 kb on + strandat 210.691 kb on + strand, within GFF4425at 210.750 kb on + strand, within GFF4425at 210.786 kb on + strand, within GFF4425at 211.190 kb on + strandat 211.219 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
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208,628 - GFF4423 0.53 -2.1
208,746 - GFF4423 0.60 -0.9
208,763 + GFF4423 0.61 +0.2
208,846 - GFF4423 0.65 -1.0
209,076 + GFF4423 0.78 -1.1
209,076 + GFF4423 0.78 +1.7
209,078 + GFF4423 0.78 -1.2
209,104 - GFF4423 0.80 +2.5
209,148 + GFF4423 0.82 -0.5
209,156 - GFF4423 0.83 +0.1
209,175 - GFF4423 0.84 -0.3
209,215 + GFF4423 0.86 +0.1
209,215 + GFF4423 0.86 +0.4
209,270 + GFF4423 0.89 -0.5
209,454 + -0.1
209,898 - GFF4424 0.54 +1.0
209,988 - GFF4424 0.66 +1.1
210,008 + GFF4424 0.69 +0.8
210,009 - GFF4424 0.69 +0.5
210,195 + +0.1
210,337 + -0.4
210,691 + GFF4425 0.39 +1.8
210,750 + GFF4425 0.45 +0.5
210,786 + GFF4425 0.49 +0.0
211,190 + -1.0
211,219 - -2.5

Or see this region's nucleotide sequence