Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4349 and GFF4350 are separated by 7 nucleotides GFF4350 and GFF4351 are separated by 122 nucleotides GFF4351 and GFF4352 are separated by 346 nucleotides GFF4352 and GFF4353 are separated by 138 nucleotides
GFF4349 - Hypothetical lipoprotein YajG precursor, at 130,331 to 130,963
GFF4349
GFF4350 - hypothetical protein, at 130,971 to 131,090
GFF4350
GFF4351 - Cell division protein BolA, at 131,213 to 131,530
GFF4351
GFF4352 - Cell division trigger factor (EC 5.2.1.8), at 131,877 to 133,175
GFF4352
GFF4353 - ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92), at 133,314 to 134,045
GFF4353
Position (kb) 131
132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 131.013 kb on - strand, within GFF4350 at 131.166 kb on + strand at 131.167 kb on - strand at 131.220 kb on + strand at 131.220 kb on + strand at 131.221 kb on - strand at 131.233 kb on - strand at 131.393 kb on + strand, within GFF4351 at 131.427 kb on + strand, within GFF4351 at 131.428 kb on - strand, within GFF4351 at 131.428 kb on - strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.447 kb on - strand, within GFF4351 at 131.447 kb on - strand, within GFF4351 at 131.447 kb on - strand, within GFF4351 at 131.450 kb on + strand, within GFF4351 at 131.450 kb on + strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.453 kb on - strand, within GFF4351 at 131.482 kb on + strand, within GFF4351 at 131.485 kb on + strand, within GFF4351 at 131.548 kb on + strand at 131.548 kb on + strand at 131.549 kb on - strand at 131.740 kb on + strand at 131.741 kb on - strand at 131.741 kb on - strand at 131.742 kb on + strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.820 kb on + strand at 131.996 kb on + strand at 131.996 kb on + strand at 131.997 kb on - strand at 132.151 kb on - strand, within GFF4352 at 132.190 kb on - strand, within GFF4352 at 132.311 kb on + strand, within GFF4352 at 132.538 kb on - strand, within GFF4352 at 132.781 kb on + strand, within GFF4352 at 133.049 kb on + strand at 133.067 kb on + strand at 133.067 kb on + strand at 133.186 kb on + strand at 133.518 kb on - strand, within GFF4353 at 133.522 kb on - strand, within GFF4353 at 133.577 kb on + strand, within GFF4353 at 133.577 kb on + strand, within GFF4353 at 133.693 kb on - strand, within GFF4353 at 133.710 kb on - strand, within GFF4353 at 133.737 kb on - strand, within GFF4353 at 133.964 kb on + strand, within GFF4353 at 134.007 kb on + strand at 134.007 kb on + strand at 134.007 kb on + strand at 134.071 kb on + strand at 134.129 kb on - strand at 134.132 kb on + strand at 134.136 kb on + strand at 134.136 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 131,013 - GFF4350 0.35 -0.7 131,166 + -0.2 131,167 - -0.0 131,220 + -0.1 131,220 + +0.1 131,221 - +0.7 131,233 - -0.0 131,393 + GFF4351 0.57 -0.3 131,427 + GFF4351 0.67 +0.7 131,428 - GFF4351 0.68 -0.4 131,428 - GFF4351 0.68 -0.4 131,446 + GFF4351 0.73 -0.2 131,446 + GFF4351 0.73 -0.9 131,446 + GFF4351 0.73 +0.2 131,446 + GFF4351 0.73 -0.9 131,447 - GFF4351 0.74 +1.5 131,447 - GFF4351 0.74 +0.6 131,447 - GFF4351 0.74 -0.8 131,450 + GFF4351 0.75 -2.5 131,450 + GFF4351 0.75 -1.6 131,451 - GFF4351 0.75 -0.5 131,451 - GFF4351 0.75 -0.5 131,451 - GFF4351 0.75 -0.2 131,451 - GFF4351 0.75 -1.9 131,453 - GFF4351 0.75 +0.8 131,482 + GFF4351 0.85 -0.3 131,485 + GFF4351 0.86 -0.9 131,548 + -0.9 131,548 + +0.6 131,549 - +0.2 131,740 + +0.7 131,741 - +1.6 131,741 - +1.8 131,742 + -0.9 131,743 - +1.5 131,743 - +2.0 131,820 + -0.3 131,996 + -2.2 131,996 + +1.3 131,997 - +0.1 132,151 - GFF4352 0.21 +1.2 132,190 - GFF4352 0.24 +1.1 132,311 + GFF4352 0.33 +1.6 132,538 - GFF4352 0.51 +1.1 132,781 + GFF4352 0.70 +0.8 133,049 + +1.0 133,067 + +0.9 133,067 + +0.3 133,186 + -0.2 133,518 - GFF4353 0.28 +0.2 133,522 - GFF4353 0.28 -2.2 133,577 + GFF4353 0.36 -2.8 133,577 + GFF4353 0.36 -2.7 133,693 - GFF4353 0.52 -2.8 133,710 - GFF4353 0.54 -2.5 133,737 - GFF4353 0.58 -3.4 133,964 + GFF4353 0.89 -1.1 134,007 + -2.5 134,007 + -0.9 134,007 + -3.6 134,071 + -1.2 134,129 - -1.8 134,132 + -1.6 134,136 + -1.2 134,136 + -0.5
Or see this region's nucleotide sequence