Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4342 and GFF4343 are separated by 14 nucleotides GFF4343 and GFF4344 overlap by 1 nucleotides GFF4344 and GFF4345 overlap by 11 nucleotides
GFF4342 - Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB, at 123,566 to 124,453
GFF4342
GFF4343 - Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-), at 124,468 to 124,797
GFF4343
GFF4344 - Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-), at 124,797 to 125,411
GFF4344
GFF4345 - Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-), at 125,401 to 127,392
GFF4345
Position (kb) 124
125
126 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 123.823 kb on - strand, within GFF4342 at 124.078 kb on - strand, within GFF4342 at 124.078 kb on - strand, within GFF4342 at 124.086 kb on + strand, within GFF4342 at 124.194 kb on - strand, within GFF4342 at 124.289 kb on - strand, within GFF4342 at 124.289 kb on - strand, within GFF4342 at 124.334 kb on - strand, within GFF4342 at 124.336 kb on - strand, within GFF4342 at 124.488 kb on - strand at 124.488 kb on - strand at 124.503 kb on + strand, within GFF4343 at 124.504 kb on - strand, within GFF4343 at 124.650 kb on + strand, within GFF4343 at 124.732 kb on - strand, within GFF4343 at 124.785 kb on + strand at 124.799 kb on - strand at 124.830 kb on + strand at 125.075 kb on + strand, within GFF4344 at 125.118 kb on - strand, within GFF4344 at 125.144 kb on + strand, within GFF4344 at 125.272 kb on + strand, within GFF4344 at 125.273 kb on - strand, within GFF4344 at 125.277 kb on - strand, within GFF4344 at 125.431 kb on - strand at 125.484 kb on - strand at 125.484 kb on - strand at 125.487 kb on - strand at 125.744 kb on + strand, within GFF4345 at 125.978 kb on + strand, within GFF4345 at 125.978 kb on + strand, within GFF4345 at 125.979 kb on - strand, within GFF4345 at 125.989 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.088 kb on - strand, within GFF4345 at 126.281 kb on + strand, within GFF4345
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 123,823 - GFF4342 0.29 -1.1 124,078 - GFF4342 0.58 +0.4 124,078 - GFF4342 0.58 -1.3 124,086 + GFF4342 0.59 -1.4 124,194 - GFF4342 0.71 -2.1 124,289 - GFF4342 0.81 -2.4 124,289 - GFF4342 0.81 -0.4 124,334 - GFF4342 0.86 -0.6 124,336 - GFF4342 0.87 -1.2 124,488 - -2.9 124,488 - -1.7 124,503 + GFF4343 0.11 -1.3 124,504 - GFF4343 0.11 -0.9 124,650 + GFF4343 0.55 -1.4 124,732 - GFF4343 0.80 +0.4 124,785 + -0.9 124,799 - -0.7 124,830 + +0.9 125,075 + GFF4344 0.45 -1.7 125,118 - GFF4344 0.52 -2.0 125,144 + GFF4344 0.56 -1.0 125,272 + GFF4344 0.77 -2.8 125,273 - GFF4344 0.77 -1.3 125,277 - GFF4344 0.78 -2.4 125,431 - +0.8 125,484 - -2.5 125,484 - +0.5 125,487 - -0.4 125,744 + GFF4345 0.17 -1.4 125,978 + GFF4345 0.29 -1.5 125,978 + GFF4345 0.29 -2.7 125,979 - GFF4345 0.29 -1.7 125,989 - GFF4345 0.30 +0.5 126,081 - GFF4345 0.34 -1.0 126,081 - GFF4345 0.34 +3.4 126,081 - GFF4345 0.34 -0.3 126,081 - GFF4345 0.34 -0.1 126,088 - GFF4345 0.34 -1.8 126,281 + GFF4345 0.44 -0.7
Or see this region's nucleotide sequence