Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF429 and GFF430 are separated by 3 nucleotides GFF430 and GFF431 are separated by 11 nucleotides
GFF429 - L-xylulose/3-keto-L-gulonate kinase (EC 2.7.1.-), at 150,619 to 152,115
GFF429
GFF430 - 2,3-diketo-L-gulonate-binding periplasmic protein yiaO precursor, at 152,119 to 153,105
GFF430
GFF431 - 2,3-diketo-L-gulonate TRAP transporter large permease protein yiaN, at 153,117 to 154,394
GFF431
Position (kb)
152
153
154 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 151.230 kb on + strand, within GFF429 at 151.230 kb on + strand, within GFF429 at 151.232 kb on + strand, within GFF429 at 151.233 kb on - strand, within GFF429 at 151.260 kb on - strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.544 kb on + strand, within GFF429 at 151.566 kb on - strand, within GFF429 at 151.631 kb on + strand, within GFF429 at 151.658 kb on - strand, within GFF429 at 151.667 kb on + strand, within GFF429 at 151.700 kb on - strand, within GFF429 at 151.700 kb on - strand, within GFF429 at 151.832 kb on - strand, within GFF429 at 152.005 kb on + strand at 152.036 kb on - strand at 152.058 kb on - strand at 152.071 kb on + strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.182 kb on - strand at 152.309 kb on + strand, within GFF430 at 152.382 kb on + strand, within GFF430 at 152.433 kb on - strand, within GFF430 at 152.433 kb on - strand, within GFF430 at 152.441 kb on - strand, within GFF430 at 152.475 kb on + strand, within GFF430 at 152.475 kb on + strand, within GFF430 at 152.476 kb on - strand, within GFF430 at 152.565 kb on + strand, within GFF430 at 152.797 kb on + strand, within GFF430 at 152.797 kb on + strand, within GFF430 at 152.798 kb on - strand, within GFF430 at 152.798 kb on - strand, within GFF430 at 153.305 kb on + strand, within GFF431 at 153.367 kb on - strand, within GFF431 at 153.370 kb on - strand, within GFF431 at 153.377 kb on + strand, within GFF431 at 153.451 kb on - strand, within GFF431 at 153.504 kb on + strand, within GFF431 at 153.632 kb on + strand, within GFF431 at 153.839 kb on + strand, within GFF431 at 153.926 kb on + strand, within GFF431 at 153.927 kb on - strand, within GFF431 at 153.948 kb on - strand, within GFF431 at 154.004 kb on - strand, within GFF431
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 151,230 + GFF429 0.41 -1.0 151,230 + GFF429 0.41 -0.4 151,232 + GFF429 0.41 -1.5 151,233 - GFF429 0.41 +0.1 151,260 - GFF429 0.43 +0.6 151,265 + GFF429 0.43 +1.4 151,265 + GFF429 0.43 -0.7 151,265 + GFF429 0.43 -0.5 151,544 + GFF429 0.62 +0.6 151,566 - GFF429 0.63 +0.0 151,631 + GFF429 0.68 -0.8 151,658 - GFF429 0.69 -1.3 151,667 + GFF429 0.70 -0.3 151,700 - GFF429 0.72 -1.8 151,700 - GFF429 0.72 +1.4 151,832 - GFF429 0.81 +0.8 152,005 + -0.2 152,036 - -0.1 152,058 - +0.4 152,071 + -0.8 152,121 - +0.3 152,121 - -0.6 152,121 - +1.6 152,121 - -0.1 152,121 - +0.7 152,182 - -0.2 152,309 + GFF430 0.19 +2.2 152,382 + GFF430 0.27 +0.1 152,433 - GFF430 0.32 +1.9 152,433 - GFF430 0.32 +0.4 152,441 - GFF430 0.33 +0.8 152,475 + GFF430 0.36 +0.9 152,475 + GFF430 0.36 +0.5 152,476 - GFF430 0.36 +0.2 152,565 + GFF430 0.45 +0.7 152,797 + GFF430 0.69 +0.0 152,797 + GFF430 0.69 -0.3 152,798 - GFF430 0.69 -2.6 152,798 - GFF430 0.69 +0.6 153,305 + GFF431 0.15 -0.3 153,367 - GFF431 0.20 +1.5 153,370 - GFF431 0.20 +0.5 153,377 + GFF431 0.20 +0.2 153,451 - GFF431 0.26 +0.2 153,504 + GFF431 0.30 +0.1 153,632 + GFF431 0.40 -1.4 153,839 + GFF431 0.56 +0.3 153,926 + GFF431 0.63 -2.8 153,927 - GFF431 0.63 +0.1 153,948 - GFF431 0.65 +0.1 154,004 - GFF431 0.69 +0.5
Or see this region's nucleotide sequence