Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4294 and GFF4295 are separated by 41 nucleotides GFF4295 and GFF4296 are separated by 41 nucleotides GFF4296 and GFF4297 are separated by 69 nucleotides GFF4297 and GFF4298 are separated by 212 nucleotides GFF4298 and GFF4299 are separated by 46 nucleotides
GFF4294 - Exonuclease SbcD, at 79,590 to 80,792
GFF4294
GFF4295 - hypothetical protein, at 80,834 to 80,965
GFF4295
GFF4296 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 81,007 to 81,696
GFF4296
GFF4297 - Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3), at 81,766 to 83,061
GFF4297
GFF4298 - hypothetical protein, at 83,274 to 83,423
GFF4298
GFF4299 - Branched-chain amino acid transport system carrier protein, at 83,470 to 84,789
GFF4299
Position (kb) 81
82
83
84 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 80.793 kb on - strand at 80.793 kb on - strand at 80.827 kb on - strand at 80.828 kb on + strand at 80.828 kb on + strand at 80.829 kb on - strand at 80.829 kb on - strand at 80.965 kb on + strand at 80.982 kb on + strand at 80.987 kb on - strand at 80.987 kb on - strand at 81.109 kb on + strand, within GFF4296 at 81.110 kb on - strand, within GFF4296 at 81.234 kb on + strand, within GFF4296 at 81.311 kb on - strand, within GFF4296 at 81.543 kb on - strand, within GFF4296 at 81.713 kb on - strand at 81.758 kb on + strand at 81.759 kb on - strand at 81.831 kb on + strand at 81.994 kb on + strand, within GFF4297 at 82.001 kb on + strand, within GFF4297 at 82.191 kb on + strand, within GFF4297 at 82.310 kb on - strand, within GFF4297 at 82.310 kb on - strand, within GFF4297 at 82.311 kb on + strand, within GFF4297 at 82.498 kb on - strand, within GFF4297 at 82.516 kb on + strand, within GFF4297 at 82.604 kb on + strand, within GFF4297 at 82.605 kb on - strand, within GFF4297 at 82.669 kb on - strand, within GFF4297 at 82.719 kb on - strand, within GFF4297 at 82.719 kb on - strand, within GFF4297 at 82.782 kb on + strand, within GFF4297 at 82.885 kb on + strand, within GFF4297 at 83.109 kb on - strand at 83.277 kb on + strand at 83.551 kb on - strand at 83.551 kb on - strand at 83.842 kb on + strand, within GFF4299 at 84.010 kb on + strand, within GFF4299
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 80,793 - -1.7 80,793 - -1.5 80,827 - +1.3 80,828 + +0.0 80,828 + +0.3 80,829 - +0.3 80,829 - -0.5 80,965 + +1.5 80,982 + +0.9 80,987 - +0.7 80,987 - -2.9 81,109 + GFF4296 0.15 -1.4 81,110 - GFF4296 0.15 -0.5 81,234 + GFF4296 0.33 -2.7 81,311 - GFF4296 0.44 -0.2 81,543 - GFF4296 0.78 +0.9 81,713 - +0.6 81,758 + +0.9 81,759 - -0.2 81,831 + +1.1 81,994 + GFF4297 0.18 -1.2 82,001 + GFF4297 0.18 -0.2 82,191 + GFF4297 0.33 -0.7 82,310 - GFF4297 0.42 -0.1 82,310 - GFF4297 0.42 +0.1 82,311 + GFF4297 0.42 -1.2 82,498 - GFF4297 0.56 +0.1 82,516 + GFF4297 0.58 +0.4 82,604 + GFF4297 0.65 -0.8 82,605 - GFF4297 0.65 -0.1 82,669 - GFF4297 0.70 +0.2 82,719 - GFF4297 0.74 +1.1 82,719 - GFF4297 0.74 +0.3 82,782 + GFF4297 0.78 -0.4 82,885 + GFF4297 0.86 -0.3 83,109 - -0.4 83,277 + +0.5 83,551 - +0.6 83,551 - +0.1 83,842 + GFF4299 0.28 +0.3 84,010 + GFF4299 0.41 -1.9
Or see this region's nucleotide sequence