Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4187 and GFF4188 overlap by 4 nucleotides GFF4188 and GFF4189 are separated by 5 nucleotides
GFF4187 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 2,135 to 3,424
GFF4187
GFF4188 - Response regulator of zinc sigma-54-dependent two-component system, at 3,421 to 4,746
GFF4188
GFF4189 - Sensor protein of zinc sigma-54-dependent two-component system, at 4,752 to 6,149
GFF4189
Position (kb)
3
4
5 Strain fitness (log2 ratio)
-2
-1
0
1 at 2.926 kb on + strand, within GFF4187 at 2.927 kb on - strand, within GFF4187 at 2.930 kb on + strand, within GFF4187 at 2.931 kb on - strand, within GFF4187 at 3.036 kb on + strand, within GFF4187 at 3.036 kb on + strand, within GFF4187 at 3.036 kb on + strand, within GFF4187 at 3.037 kb on - strand, within GFF4187 at 3.130 kb on - strand, within GFF4187 at 3.286 kb on - strand, within GFF4187 at 3.457 kb on - strand at 3.564 kb on - strand, within GFF4188 at 3.621 kb on - strand, within GFF4188 at 3.693 kb on - strand, within GFF4188 at 3.781 kb on + strand, within GFF4188 at 3.849 kb on - strand, within GFF4188 at 3.852 kb on - strand, within GFF4188 at 3.935 kb on - strand, within GFF4188 at 3.946 kb on - strand, within GFF4188 at 4.144 kb on - strand, within GFF4188 at 4.144 kb on - strand, within GFF4188 at 4.685 kb on - strand at 4.702 kb on + strand at 4.703 kb on - strand at 4.703 kb on - strand at 4.754 kb on - strand at 4.907 kb on - strand, within GFF4189 at 5.102 kb on + strand, within GFF4189 at 5.103 kb on - strand, within GFF4189 at 5.103 kb on - strand, within GFF4189 at 5.338 kb on + strand, within GFF4189 at 5.471 kb on - strand, within GFF4189 at 5.496 kb on + strand, within GFF4189 at 5.543 kb on + strand, within GFF4189 at 5.547 kb on - strand, within GFF4189 at 5.598 kb on - strand, within GFF4189 at 5.598 kb on - strand, within GFF4189
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 2,926 + GFF4187 0.61 -0.6 2,927 - GFF4187 0.61 +0.7 2,930 + GFF4187 0.62 +1.0 2,931 - GFF4187 0.62 -0.8 3,036 + GFF4187 0.70 +0.0 3,036 + GFF4187 0.70 +0.2 3,036 + GFF4187 0.70 +0.6 3,037 - GFF4187 0.70 -2.0 3,130 - GFF4187 0.77 -0.2 3,286 - GFF4187 0.89 -0.5 3,457 - -0.6 3,564 - GFF4188 0.11 +0.5 3,621 - GFF4188 0.15 -0.7 3,693 - GFF4188 0.21 -0.2 3,781 + GFF4188 0.27 -0.5 3,849 - GFF4188 0.32 +0.7 3,852 - GFF4188 0.33 +0.4 3,935 - GFF4188 0.39 +1.7 3,946 - GFF4188 0.40 +1.0 4,144 - GFF4188 0.55 -1.1 4,144 - GFF4188 0.55 +0.1 4,685 - +0.3 4,702 + +0.6 4,703 - +0.1 4,703 - +0.1 4,754 - -0.7 4,907 - GFF4189 0.11 -2.2 5,102 + GFF4189 0.25 +0.7 5,103 - GFF4189 0.25 -2.4 5,103 - GFF4189 0.25 +0.4 5,338 + GFF4189 0.42 +0.7 5,471 - GFF4189 0.51 -0.1 5,496 + GFF4189 0.53 +0.7 5,543 + GFF4189 0.57 +0.0 5,547 - GFF4189 0.57 +0.7 5,598 - GFF4189 0.61 -0.3 5,598 - GFF4189 0.61 +0.3
Or see this region's nucleotide sequence