Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4137 and GFF4138 overlap by 4 nucleotides GFF4138 and GFF4139 overlap by 4 nucleotides GFF4139 and GFF4140 are separated by 112 nucleotides GFF4140 and GFF4141 are separated by 221 nucleotides GFF4141 and GFF4142 are separated by 83 nucleotides
GFF4137 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 20,099 to 21,079
GFF4137
GFF4138 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 21,076 to 21,450
GFF4138
GFF4139 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 21,447 to 21,968
GFF4139
GFF4140 - putative cytoplasmic protein, at 22,081 to 22,365
GFF4140
GFF4141 - FIG01047227: hypothetical protein, at 22,587 to 22,748
GFF4141
GFF4142 - FIG01047293: hypothetical protein, at 22,832 to 22,993
GFF4142
Position (kb) 21
22 Strain fitness (log2 ratio) -2
-1
0
1
2 at 20.662 kb on + strand, within GFF4137 at 20.663 kb on - strand, within GFF4137 at 20.742 kb on - strand, within GFF4137 at 20.760 kb on + strand, within GFF4137 at 20.760 kb on + strand, within GFF4137 at 20.760 kb on + strand, within GFF4137 at 20.761 kb on - strand, within GFF4137 at 20.761 kb on - strand, within GFF4137 at 20.875 kb on + strand, within GFF4137 at 21.038 kb on - strand at 21.064 kb on + strand at 21.065 kb on - strand at 21.255 kb on - strand, within GFF4138 at 21.429 kb on + strand at 21.488 kb on - strand at 21.519 kb on + strand, within GFF4139 at 21.520 kb on - strand, within GFF4139 at 21.703 kb on - strand, within GFF4139 at 22.083 kb on - strand at 22.158 kb on - strand, within GFF4140 at 22.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 20,662 + GFF4137 0.57 -1.3 20,663 - GFF4137 0.57 -0.1 20,742 - GFF4137 0.66 +1.2 20,760 + GFF4137 0.67 +0.2 20,760 + GFF4137 0.67 +2.0 20,760 + GFF4137 0.67 +0.3 20,761 - GFF4137 0.67 +0.5 20,761 - GFF4137 0.67 -2.0 20,875 + GFF4137 0.79 -0.1 21,038 - -0.9 21,064 + +0.3 21,065 - +0.2 21,255 - GFF4138 0.48 -0.2 21,429 + +1.1 21,488 - +0.8 21,519 + GFF4139 0.14 +0.8 21,520 - GFF4139 0.14 +0.3 21,703 - GFF4139 0.49 +1.4 22,083 - -0.6 22,158 - GFF4140 0.27 +0.3 22,492 + +1.5
Or see this region's nucleotide sequence