Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4035 and GFF3986 are separated by 100 nucleotides GFF3986 and GFF4036 are separated by 88 nucleotides GFF4036 and GFF3987 are separated by 302 nucleotides GFF3987 and GFF3988 are separated by 306 nucleotides
GFF4035 - tRNA-Asn-GTT, at 261,529 to 261,604
GFF4035
GFF3986 - FIG01220476: hypothetical protein, at 261,705 to 262,502
GFF3986
GFF4036 - tRNA-Ser-CGA, at 262,591 to 262,680
GFF4036
GFF3987 - FIG01045245: hypothetical protein, at 262,983 to 263,144
GFF3987
GFF3988 - Error-prone repair protein UmuD, at 263,451 to 263,690
GFF3988
Position (kb) 262
263 Strain fitness (log2 ratio) -1
0
1
2 at 261.706 kb on + strand at 261.900 kb on + strand, within GFF3986 at 261.900 kb on + strand, within GFF3986 at 261.901 kb on - strand, within GFF3986 at 262.156 kb on - strand, within GFF3986 at 262.183 kb on - strand, within GFF3986 at 262.302 kb on - strand, within GFF3986 at 262.568 kb on + strand at 262.637 kb on + strand, within GFF4036 at 262.842 kb on + strand at 262.842 kb on + strand at 262.978 kb on + strand at 262.978 kb on + strand at 262.979 kb on - strand at 262.979 kb on - strand at 263.004 kb on - strand, within GFF3987 at 263.004 kb on - strand, within GFF3987 at 263.044 kb on + strand, within GFF3987 at 263.044 kb on + strand, within GFF3987 at 263.198 kb on - strand at 263.496 kb on - strand, within GFF3988 at 263.496 kb on - strand, within GFF3988 at 263.528 kb on - strand, within GFF3988 at 263.590 kb on + strand, within GFF3988 at 263.621 kb on - strand, within GFF3988 at 263.670 kb on - strand at 263.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 261,706 + +0.6 261,900 + GFF3986 0.24 +0.0 261,900 + GFF3986 0.24 +2.4 261,901 - GFF3986 0.25 -1.2 262,156 - GFF3986 0.57 +0.1 262,183 - GFF3986 0.60 +1.3 262,302 - GFF3986 0.75 +0.7 262,568 + -0.5 262,637 + GFF4036 0.51 +0.9 262,842 + -0.4 262,842 + -0.1 262,978 + +0.1 262,978 + -0.3 262,979 - -0.5 262,979 - -0.6 263,004 - GFF3987 0.13 +0.4 263,004 - GFF3987 0.13 +0.6 263,044 + GFF3987 0.38 +0.5 263,044 + GFF3987 0.38 +1.1 263,198 - +0.7 263,496 - GFF3988 0.19 +0.4 263,496 - GFF3988 0.19 +0.2 263,528 - GFF3988 0.32 +0.8 263,590 + GFF3988 0.58 +0.6 263,621 - GFF3988 0.71 +0.5 263,670 - -0.8 263,670 - +0.2
Or see this region's nucleotide sequence